PPP2R3A (NM_181897) Human Tagged ORF Clone

CAT#: RC215798

PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405582) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215798 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R3A
• Synonyms: PPP2R3; PR72; PR130
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215798 representing NM_181897
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATCAAGGAAACATCTCTACGAAGGGACCCGGATTTAAGGGGAGAGCTAGCTTTCCTGGCAAGGG
  GCTGTGATTTTGTTCTCCCTTCACGGTTTAAGAAGCGGCTGAAGTCATTTCAGCAGACACAGATTCAAAA
  TAAACCAGAAAAGAAACCTGGAACACCACTCCCACCTCCAGCCACCTCTCCAAGTAGTCCCCGACCTCTC
  TCCCCGGTTCCCCATGTGAATAATGTTGTGAATGCGCCATTGTCCATAAACATTCCACGGTTCTACTTTC
  CTGAAGGACTCCCAGATACCTGTAGTAATCATGAACAAACTCTAAGCAGAATTGAAACTGCTTTCATGGA
  TATTGAAGAACAGAAAGCAGACATTTATGAAATGGGGAAAATTGCAAAGGTCTGTGGCTGTCCTCTCTAT
  TGGAAAGCCCCCATGTTCAGGGCTGCAGGGGGAGAGAAGACAGGATTTGTGACAGCACAGTCATTCATTG
  CCATGTGGAGAAAGTTGCTGAATAACCATCATGATGATGCCTCTAAATTCATCTGTCTTCTAGCAAAGCC
  CAACTGCAGCTCTCTAGAACAGGAGGATTTCATCCCTCTACTTCAGGATGTGGTGGATACCCACCCTGGT
  CTCACGTTCCTGAAAGATGCTCCAGAATTCCACTCCCGCTACATCACCACGGTTATTCAGAGAATATTCT
  ACACAGTCAACAGATCTTGGAGTGGAAAAATTACTTCGACAGAGATAAGAAAAAGCAACTTTTTGCAAAC
  CCTAGCACTTTTGGAAGAAGAGGAAGATATAAACCAAATTACAGATTACTTCTCCTATGAACATTTCTAT
  GTTATTTATTGTAAATTCTGGGAACTAGATACTGATCACGACCTCTACATCAGCCAGGCCGATCTGTCTC
  GATACAATGACCAGGCTTCATCAAGCAGGATTATTGAAAGGATATTCTCTGGTGCAGTAACAAGGGGAAA
  AACAATACAGAAAGAGGGAAGAATGAGCTATGCAGATTTTGTTTGGTTTTTGATCTCTGAAGAAGACAAA
  AGGAATCCTACCAGCATTGAGTATTGGTTCCGCTGCATGGATGTGGATGGAGACGGTGTACTCTCCATGT
  ATGAGCTGGAGTACTTCTATGAGGAGCAGTGTGAACGGATGGAAGCCATGGGAATTGAGCCCTTGCCATT
  CCATGATTTACTGTGCCAGATGCTTGACCTAGTGAAGCCAGCTGTTGATGGCAAAATAACTCTAAGAGAT
  CTGAAGAGGTGCAGAATGGCTCACATCTTCTATGACACTTTCTTTAATCTGGAGAAATACTTAGACCATG
  AACAGAGAGATCCCTTTGCGGTCCAGAAGGATGTTGAGAACGATGGGCCTGAGCCCTCAGACTGGGACCG
  GTTTGCCGCTGAGGAGTATGAGACGCTTGTTGCAGAGGAATCTGCCCAAGCACAATTCCAGGAAGGCTTT
  GAAGATTATGAAACAGATGAACCTGCCTCTCCCTCTGAATTTGGAAACAAAAGCAATAAAATATTAAGTG
  CAAGCCTTCCAGAGAAATGTGGAAAGCTTCAATCAGTGGATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215798 representing NM_181897
  Red=Cloning site Green=Tags(s)
  
  MMIKETSLRRDPDLRGELAFLARGCDFVLPSRFKKRLKSFQQTQIQNKPEKKPGTPLPPPATSPSSPRPL
  SPVPHVNNVVNAPLSINIPRFYFPEGLPDTCSNHEQTLSRIETAFMDIEEQKADIYEMGKIAKVCGCPLY
  WKAPMFRAAGGEKTGFVTAQSFIAMWRKLLNNHHDDASKFICLLAKPNCSSLEQEDFIPLLQDVVDTHPG
  LTFLKDAPEFHSRYITTVIQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFY
  VIYCKFWELDTDHDLYISQADLSRYNDQASSSRIIERIFSGAVTRGKTIQKEGRMSYADFVWFLISEEDK
  RNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPAVDGKITLRD
  LKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENDGPEPSDWDRFAAEEYETLVAEESAQAQFQEGF
  EDYETDEPASPSEFGNKSNKILSASLPEKCGKLQSVDEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181897
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181897.1, NM_181897.2, NP_871626.1
• RefSeq Size: 4664 bp
• RefSeq ORF: 1590 bp
• Locus ID: 5523
• Cytogenetics: 3q22.2-q22.3
• Protein Families: Druggable Genome, Phosphatase
• MW: 60.9 kDa
• Gene Summary: 'This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010]'