GJB3 (NM_024009) Human Tagged ORF Clone
CAT#: RC215842
GJB3 (Myc-DDK-tagged)-Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1
"NM_024009" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GJB3 |
Synonyms | CX31; DFNA2; DFNA2B; EKV; EKVP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215842 representing NM_024009
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGAAGACACTCCAGGCCCTACTGAGCGGTGTGAACAAGTACTCCACAGCGTTCGGGCGCATCT GGCTGTCCGTGGTGTTCGTCTTCCGGGTGCTGGTATACGTGGTGGCTGCAGAGCGCGTGTGGGGGGATGA GCAGAAGGACTTTGACTGCAACACCAAGCAGCCCGGCTGCACCAACGTCTGCTACGACAACTACTTCCCC ATCTCCAACATCCGCCTCTGGGCCCTGCAGCTCATCTTCGTCACATGCCCCTCGCTGCTGGTCATCCTGC ACGTGGCCTACCGTGAGGAGCGGGAGCGCCGGCACCGCCAGAAACACGGGGACCAGTGCGCCAAGCTGTA CGACAACGCAGGCAAGAAGCACGGAGGCCTGTGGTGGACCTACCTGTTCAGCCTCATCTTCAAGCTCATC ATTGAGTTCCTCTTCCTCTACCTGCTGCACACTCTCTGGCATGGCTTCAATATGCCGCGCCTGGTGCAGT GTGCCAACGTGGCCCCCTGCCCCAACATCGTGGACTGCTACATTGCCCGACCTACCGAGAAGAAAATCTT CACCTACTTCATGGTGGGCGCCTCCGCCGTCTGCATCGTACTCACCATCTGTGAGCTCTGCTACCTCATC TGCCACAGGGTCCTGCGAGGCCTGCACAAGGACAAGCCTCGAGGGGGTTGCAGCCCCTCGTCCTCCGCCA GCCGAGCTTCCACCTGCCGCTGCCACCACAAGCTGGTGGAGGCTGGGGAGGTGGATCCAGACCCAGGCAA TAACAAGCTGCAGGCTTCAGCACCCAACCTGACCCCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215842 representing NM_024009
Red=Cloning site Green=Tags(s) MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFP ISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLI IEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLI CHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024009 |
ORF Size | 810 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024009.1, NM_024009.2, NP_076872.1 |
RefSeq Size | 2220 bp |
RefSeq ORF | 813 bp |
Locus ID | 2707 |
Cytogenetics | 1p34.3 |
Domains | CNX |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
MW | 30.6 kDa |
Gene Summary | 'This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112319 | GJB3 (untagged)-Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1 |
USD 310.00 |
|
RG215842 | GJB3 (GFP-tagged) - Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1 |
USD 460.00 |
|
RC215842L1 | Lenti ORF clone of Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC215842L2 | Lenti ORF clone of Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC215842L3 | Lenti ORF clone of Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC215842L4 | Lenti ORF clone of Human gap junction protein, beta 3, 31kDa (GJB3), transcript variant 1, mGFP tagged |
USD 620.00 |
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