CGK2 (PRKG2) (NM_006259) Human Tagged ORF Clone

CAT#: RC215879

PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401884) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215879 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRKG2 protein (Cat# TP315879). The protein was produced from HEK293T cells transfected with PRKG2 cDNA clone (Cat# RC215879) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKG2
• Synonyms: cGK2; cGKII; PKG2; PRKGR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215879 representing NM_006259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAACCTCACCACTG
  ATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAGGATGCTGAGATCCAGGAGCG
  GGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAGCAGACTGTGGCCATTGCTGAACTCACAGAG
  GAGCTCCAGAACAAGTGCATCCAGCTGAACAAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGC
  TTCAGGCCTCTCCAGATAAAGTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAG
  CAGGAGGGGAGCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACCCCCT
  GAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACAGATGCCCTTA
  ATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATGGTGGAATGCATGTATGGGAG
  AAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAACCAGGAAACCATATCTTTGTGCTGGCAGAG
  GGTCGACTAGAGGTGTTCCAAGGGGAGAAATTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGC
  TTGCCATTTTATACAATTGTACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACATGGGCACT
  AGATCGAGAGGTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAATACAGAAACTTC
  CTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATTGACTGCTTGGAAG
  TGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGAAGTACCTTTTTCATTTTGGC
  AAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCATGATCAACCACAGCTGATAAAAACACTGCAG
  AAAGGAGAATACTTTGGAGAAAAAGCTCTTATCAGTGATGATGTCAGGTCAGCTAACATTATTGCTGAAG
  AAAATGATGTTGCATGCCTGGTTATAGATCGAGAAACATTCAACCAAACTGTCGGTACATTTGAAGAGCT
  GCAAAAATACCTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAAAGACATGCGAAGCGGTCC
  ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAGGTGGCCAGAT
  TTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGCGTTGGTGGGTTCGGAAGAGT
  TGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGAAGTGTATAAGGAAGAAGCACATAGTT
  GACACCAAGCAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTG
  TGAAATTATATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGA
  GCTCTGGAGTATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCTTGTGTG
  ACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAAAACTTAATTC
  TAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAAATAGGGTCTGGACAGAAAAC
  ATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAAGTCATTCTCAACAAGGGACATGACTTCAGT
  GTGGATTTCTGGTCACTGGGAATTCTAGTGTATGAGCTCCTAACGGGCAACCCACCCTTTTCTGGGGTTG
  ACCAAATGATGACCTACAATTTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGGAAGATAACACG
  ACGACCTGAGGATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGAAATCTGAAGAAT
  GGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTGAAAGCACGGAGCC
  TTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTACTTTGACAAATATCCTCCTGA
  AAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAAGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215879 representing NM_006259
  Red=Cloning site Green=Tags(s)
  
  MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTE
  ELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPP
  EFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAE
  GRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNF
  LRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQ
  KGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRS
  MSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIV
  DTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACV
  TEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFS
  VDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKN
  GINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006259
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006259.1, NM_006259.2, NP_006250.1
• RefSeq Size: 3328 bp
• RefSeq ORF: 2289 bp
• Locus ID: 5593
• Cytogenetics: 4q21.21
• Domains: cNMP, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction
• MW: 87.3 kDa
• Gene Summary: 'This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018]'