ATP8B4 (NM_024837) Human Tagged ORF Clone

CAT#: RC216096

ATP8B4 (Myc-DDK-tagged)-Human ATPase, class I, type 8B, member 4 (ATP8B4)

Product Images

Western blot validation of overexpression lysate (Cat# LY410982) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216096 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP8B4
• Synonyms: ATPIM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216096 representing NM_024837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTGCAGTGAAAAGAAATTGCGTGAAGTGGAACGGATAGTGAAAGCCAATGACCGTGAATATAATG
  AAAAGTTCCAGTATGCGGATAATCGTATCCACACATCGAAATATAATATTCTCACCTTCTTGCCAATTAA
  TTTATTTGAACAGTTCCAAAGAGTGGCAAATGCCTATTTTCTTTGCCTTCTGATTTTACAGCTAATTCCA
  GAAATTTCCTCCTTGACCTGGTTTACCACCATTGTGCCTTTGGTCCTGGTGATAACTATGACAGCTGTCA
  AAGATGCCACAGATGACTATTTTCGCCACAAGAGTGATAATCAAGTGAATAATCGGCAGTCTGAAGTGCT
  CATCAACAGCAAACTGCAGAATGAAAAATGGATGAATGTCAAAGTGGGAGACATCATTAAATTAGAAAAT
  AACCAATTTGTTGCTGCTGATTTACTTCTCCTATCAAGTAGTGAGCCACATGGTCTCTGTTATGTTGAAA
  CTGCTGAGCTTGATGGGGAAACGAACCTAAAAGTCCGCCATGCACTATCAGTTACTTCAGAACTTGGAGC
  AGATATCAGCAGACTTGCAGGGTTTGATGGGATTGTTGTCTGTGAGGTGCCTAACAACAAGTTAGATAAA
  TTCATGGGAATCCTTTCTTGGAAAGACAGCAAGCATTCCCTCAACAATGAGAAGATAATCCTGAGAGGCT
  GCATCCTGAGAAATACCAGCTGGTGTTTTGGAATGGTTATTTTTGCAGGTCCTGACACTAAACTAATGCA
  GAATAGTGGTAAGACAAAGTTTAAAAGGACAAGCATTGATAGATTGATGAATACTCTAGTACTATGGATT
  TTTGGGTTTCTGATATGCTTGGGAATTATTCTTGCAATAGGAAATTCAATCTGGGAGAGTCAAACTGGGG
  ACCAATTCAGAACTTTCCTCTTTTGGAATGAAGGAGAGAAGAGCTCTGTGTTCTCCGGATTCTTAACATT
  CTGGTCATATATTATTATTCTCAATACAGTTGTACCCATTTCCTTATATGTGAGTGTGGAAGTAATTCGT
  CTAGGACACAGTTATTTTATAAACTGGGACCGGAAGATGTATTATTCTCGAAAAGCAATACCTGCAGTGG
  CTCGAACGACCACGCTCAATGAGGAACTGGGGCAGATTGAGTACATTTTCTCCGACAAAACGGGTACCCT
  CACTCAAAACATCATGACCTTTAAAAGATGTTCCATTAATGGGAGAATCTATGGTGAAGTACATGATGAC
  CTGGATCAGAAGACAGAAATAACTCAGGAAAAAGAGCCTGTGGATTTCTCAGTCAAATCTCAAGCGGATA
  GAGAATTTCAGTTCTTTGACCACCATCTGATGGAATCCATTAAAATGGGTGATCCCAAAGTTCATGAATT
  CCTTAGGTTACTTGCTCTCTGCCACACTGTAATGTCAGAAGAGAATAGCGCAGGAGAGCTGATTTACCAA
  GTTCAGTCACCTGATGAAGGGGCTCTAGTGACTGCCGCTAGAAATTTTGGGTTCATTTTTAAATCCCGGA
  CCCCAGAGACCATAACAATAGAAGAATTGGGAACACTAGTTACTTATCAATTACTTGCCTTTTTGGATTT
  CAACAACACCAGAAAAAGGATGTCTGTCATAGTTCGAAACCCAGAAGGACAGATAAAGCTTTATTCCAAA
  GGAGCAGATACTATTCTGTTTGAAAAACTTCATCCTTCCAATGAAGTCCTTTTGTCTTTGACGTCAGACC
  ACCTCAGTGAATTTGCAGGGGAAGGCCTTCGGACCTTGGCCATCGCATACAGAGACCTGGATGACAAGTA
  CTTTAAAGAGTGGCATAAGATGCTTGAAGATGCGAATGCTGCCACAGAAGAGAGGGATGAACGAATAGCT
  GGGCTATATGAAGAAATTGAAAGAGATTTGATGCTACTAGGTGCCACTGCTGTAGAAGATAAGTTACAGG
  AGGGTGTTATTGAAACAGTTACAAGTTTATCACTAGCCAATATTAAGATCTGGGTCCTAACAGGAGACAA
  ACAAGAAACTGCCATCAACATCGGTTATGCCTGCAACATGCTGACTGACGACATGAATGATGTGTTTGTG
  ATAGCAGGGAATAATGCTGTGGAAGTGAGAGAAGAACTCAGGAAAGCAAAACAAAATTTGTTTGGACAAA
  ACAGAAATTTTTCCAATGGCCATGTAGTTTGTGAAAAAAAGCAGCAGCTGGAGTTGGATTCTATTGTAGA
  AGAAACCATAACAGGAGATTATGCCTTAATCATAAATGGCCACAGTTTGGCTCATGCCCTAGAAAGTGAT
  GTCAAGAATGATCTCCTAGAACTTGCTTGCATGTGTAAGACTGTAATTTGCTGCAGGGTCACTCCACTCC
  AGAAAGCCCAAGTGGTAGAGCTGGTGAAGAAGTACAGAAATGCTGTTACTTTGGCCATTGGTGATGGAGC
  CAATGATGTCAGCATGATTAAAAGTGCTCACATTGGTGTTGGCATCAGCGGCCAGGAAGGATTGCAAGCA
  GTCTTAGCCAGCGACTATTCATTTGCACAGTTTAGATATCTCCAAAGGCTTCTCCTTGTTCATGGAAGGT
  GGTCTTATTTCCGAATGTGCAAATTCTTATGCTATTTCTTCTATAAGAATTTTGCATTTACACTTGTGCA
  TTTCTGGTTTGGTTTCTTCTGTGGTTTCTCAGCCCAGACTGTTTATGACCAGTGGTTCATCACCCTTTTT
  AACATTGTTTACACATCACTGCCTGTTTTAGCCATGGGGATTTTTGACCAGGATGTGAGTGACCAGAACA
  GCGTGGACTGTCCCCAGCTCTACAAACCAGGACAGCTGAATCTGCTTTTTAACAAGCGTAAATTTTTCAT
  TTGCGTGTTGCATGGAATCTACACCTCATTAGTCCTTTTCTTCATCCCCTATGGGGCCTTTTACAACGTG
  GCTGGAGAAGATGGGCAACATATTGCTGACTACCAGTCCTTTGCAGTTACCATGGCCACATCTTTGGTCA
  TTGTGGTCAGTGTGCAGATAGCCTTGGATACCAGTTACTGGACTTTCATTAATCACGTCTTCATCTGGGG
  GAGCATTGCCATTTATTTCTCCATTTTATTTACAATGCACAGTAATGGCATCTTTGGCATCTTCCCAAAC
  CAGTTTCCATTTGTTGGTAATGCACGACATTCCCTGACCCAGAAGTGCATCTGGCTTGTAATTCTCTTAA
  CAACAGTGGCTTCAGTTATGCCAGTGGTGGCATTCAGATTTTTGAAGGTGGATTTATACCCAACCCTGAG
  TGATCAGATCCGCCGGTGGCAGAAGGCTCAAAAGAAGGCAAGGCCTCCAAGTAGCCGAAGGCCTCGGACC
  CGCAGGTCAAGCTCAAGAAGGTCTGGATATGCTTTTGCTCACCAAGAAGGCTATGGAGAGCTTATCACAT
  CTGGAAAAAATATGCGAGCTAAAAATCCACCCCCAACATCAGGGCTGGAAAAGACACATTATAATAGCAC
  TAGCTGGATTGAAAATTTATGTAAGAAAACCACAGACACCGTGAGCAGCTTTAGCCAGGATAAAACAGTG
  AAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216096 representing NM_024837
  Red=Cloning site Green=Tags(s)
  
  MFCSEKKLREVERIVKANDREYNEKFQYADNRIHTSKYNILTFLPINLFEQFQRVANAYFLCLLILQLIP
  EISSLTWFTTIVPLVLVITMTAVKDATDDYFRHKSDNQVNNRQSEVLINSKLQNEKWMNVKVGDIIKLEN
  NQFVAADLLLLSSSEPHGLCYVETAELDGETNLKVRHALSVTSELGADISRLAGFDGIVVCEVPNNKLDK
  FMGILSWKDSKHSLNNEKIILRGCILRNTSWCFGMVIFAGPDTKLMQNSGKTKFKRTSIDRLMNTLVLWI
  FGFLICLGIILAIGNSIWESQTGDQFRTFLFWNEGEKSSVFSGFLTFWSYIIILNTVVPISLYVSVEVIR
  LGHSYFINWDRKMYYSRKAIPAVARTTTLNEELGQIEYIFSDKTGTLTQNIMTFKRCSINGRIYGEVHDD
  LDQKTEITQEKEPVDFSVKSQADREFQFFDHHLMESIKMGDPKVHEFLRLLALCHTVMSEENSAGELIYQ
  VQSPDEGALVTAARNFGFIFKSRTPETITIEELGTLVTYQLLAFLDFNNTRKRMSVIVRNPEGQIKLYSK
  GADTILFEKLHPSNEVLLSLTSDHLSEFAGEGLRTLAIAYRDLDDKYFKEWHKMLEDANAATEERDERIA
  GLYEEIERDLMLLGATAVEDKLQEGVIETVTSLSLANIKIWVLTGDKQETAINIGYACNMLTDDMNDVFV
  IAGNNAVEVREELRKAKQNLFGQNRNFSNGHVVCEKKQQLELDSIVEETITGDYALIINGHSLAHALESD
  VKNDLLELACMCKTVICCRVTPLQKAQVVELVKKYRNAVTLAIGDGANDVSMIKSAHIGVGISGQEGLQA
  VLASDYSFAQFRYLQRLLLVHGRWSYFRMCKFLCYFFYKNFAFTLVHFWFGFFCGFSAQTVYDQWFITLF
  NIVYTSLPVLAMGIFDQDVSDQNSVDCPQLYKPGQLNLLFNKRKFFICVLHGIYTSLVLFFIPYGAFYNV
  AGEDGQHIADYQSFAVTMATSLVIVVSVQIALDTSYWTFINHVFIWGSIAIYFSILFTMHSNGIFGIFPN
  QFPFVGNARHSLTQKCIWLVILLTTVASVMPVVAFRFLKVDLYPTLSDQIRRWQKAQKKARPPSSRRPRT
  RRSSSRRSGYAFAHQEGYGELITSGKNMRAKNPPPTSGLEKTHYNSTSWIENLCKKTTDTVSSFSQDKTV
  KL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024837
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024837.2, NM_024837.3, NP_079113.2
• RefSeq Size: 5679
• RefSeq ORF: 3579
• Locus ID: 79895
• Protein Families: Transmembrane
• MW: 135.7 kDa
• Gene Summary: This gene encodes a member of the cation transport ATPase (P-type) family and type IV subfamily. The encoded protein is involved in phospholipid transport in the cell membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]