HYAL1 (NM_033159) Human Tagged ORF Clone

CAT#: RC216129L4

Lenti ORF clone of Human hyaluronoglucosaminidase 1 (HYAL1), transcript variant 7, mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: HYAL1
• Synonyms: HYAL-1; LUCA1; MPS9; NAT6
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC216129).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_033159
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033159.2, NP_149349.2
• RefSeq Size: 2103 bp
• RefSeq ORF: 1308 bp
• Locus ID: 3373
• Cytogenetics: 3p21.31
• Domains: Glyco_hydro_56
• Protein Families: Secreted Protein
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• MW: 48.4 kDa
• Gene Summary: 'This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'