WASF3 (NM_006646) Human Tagged ORF Clone

CAT#: RC216156

WASF3 (Myc-DDK-tagged)-Human WAS protein family, member 3 (WASF3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WASF3
• Synonyms: Brush-1; SCAR3; WAVE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216156 representing NM_006646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAAGGGATTACCA
  GCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAGCTGAGCAGTCTGAGCAAACA
  TGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAACAACTTCTACATCAGAGCAAATTCTCTTCAA
  GACAGAATTGATCGCCTTGCTGTCAAAGTCACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTACAGG
  ATATCAACATGAAAAAAGCTTTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAACAGCAT
  TCCTAATCCTGTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACACCATAC
  AGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTCTGGAAAGAAA
  AAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAGGAGCAAAAGCGTATAGATGG
  CACCACCCGTGAGGTGAAAAAGGTTAGAAAAGCCAGAAACAGGCGCCAGGAGTGGAATATGATGGCATAT
  GACAAAGAGCTTAGACCCGACAACAGGTTGTCTCAGAGTGTGTACCATGGAGCGTCTTCCGAGGGATCCC
  TGTCCCCAGATACTAGGTCACATGCATCGGACGTTACGGATTACTCTTACCCGGCTACTCCCAACCATTC
  TCTGCACCCCCAGCCTGTGACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGCCAG
  GCTGCGGAGCATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAGCAGC
  CGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCTCCAGCAGACTA
  CGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCACCTCCTCCGCCACCTCCTCCT
  GTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCATGCAGCCCCCGTTCCCTGCAT
  CAGCCAGCTCCACGCACGCAGCTCCTCCTCACCCACCCTCCACCGGGCTCCTGGTCACAGCCCCGCCACC
  CCCGGGCCCACCACCTCCCCCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCCCCAATGCAT
  GGCCCCCCAGTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCTCGAAGCGACC
  TCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAGGAGGCCAAGCG
  GGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCCGTGGAGTACAGCGACTCTGAC
  GACGACTCAGAGTTCGACGAGAACGACTGGTCCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216156 representing NM_006646
  Red=Cloning site Green=Tags(s)
  
  MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEANNFYIRANSLQ
  DRIDRLAVKVTQLDSTVEEVSLQDINMKKAFKSSTVQDQQVVSKNSIPNPVADIYNQSDKPPPLNILTPY
  RDDKKDGLKFYTDPSYFFDLWKEKMLQDTEDKRKEKRRQKEQKRIDGTTREVKKVRKARNRRQEWNMMAY
  DKELRPDNRLSQSVYHGASSEGSLSPDTRSHASDVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHGPASQ
  AAEHEYRPPSASARHMALNRPQQPPPPPPPQAPEGSQASAPMAPADYGMLPAQIIEYYNPSGPPPPPPPP
  VIPSAQTAFVSPLQMPMQPPFPASASSTHAAPPHPPSTGLLVTAPPPPGPPPPPPGPPGPGSSLSSSPMH
  GPPVAEAKRQEPAQPPISDARSDLLAAIRMGIQLKKVQEQREQEAKREPVGNDVATILSRRIAVEYSDSD
  DDSEFDENDWSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006646
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006646.1, NM_006646.2, NM_006646.3, NM_006646.4, NM_006646.5, NP_006637.2
• RefSeq Size: 4850
• RefSeq ORF: 1509
• Locus ID: 10810
• Domains: WH2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Fc gamma R-mediated phagocytosis
• MW: 55.7 kDa
• Gene Summary: This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]