ARID5A (NM_212481) Human Tagged ORF Clone

CAT#: RC216294

ARID5A (Myc-DDK-tagged)-Human AT rich interactive domain 5A (MRF1-like) (ARID5A)

Product Images

Western blot validation of overexpression lysate (Cat# LY403951) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216294 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARID5A
• Synonyms: MRF-1; MRF1; RP11-363D14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216294 representing NM_212481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCCTGTCAAAGGGAACAGGAAGCAGTCCACGGAGGGTGACGCCCTAGACCCACCTGCATCCC
  CCAAACCTGCTGGCAAGCAGAACGGAATCCAGAACCCCATCTCGCTGGAGGACTCCCCCGAGGCAGGCGG
  GGAGCGGGAGGAGGAGCAGGAGCGGGAGGAGGAGCAGGCCTTCCTGGTCAGCCTCTACAAGTTCATGAAG
  GAGCGACACACGCCCATCGAGAGGGTGCCCCATCTCGGCTTCAAGCAGATTAACCTGTGGAAGATCTACA
  AAGCAGTGGAGAAGCTGGGGGCCTATGAGCTGGTGACCGGGCGCCGCCTCTGGAAGAACGTGTACGACGA
  GCTGGGGGGCAGCCCAGGCAGCACCAGCGCGGCCACGTGCACGCGCCGCCACTACGAGAGGCTGGTCCTG
  CCATACGTGCGGCACCTGAAGGGGGAGGATGACAAGCCGCTGCCCACCTCCAAGCCCAGGAAACAGTACA
  AGATGGCTAAGGAGAACAGGGGGGATGATGGGGCCACCGAGAGGCCGAAGAAGGCCAAGGAGGAGCGGCG
  CATGGACCAGATGATGCCAGGAAAGACCAAAGCAGATGCTGCTGACCCAGCACCACTTCCCAGCCAGGAG
  CCCCCCAGGAACAGCACAGAACAGCAGGGCCTGGCCTCTGGGTCTTCTGTGTCCTTTGTGGGTGCCAGCG
  GCTGTCCTGAGGCCTACAAGCGGCTCCTATCCAGCTTCTACTGCAAGGGGACACACGGCATCATGTCACC
  ACTGGCCAAAAAGAAGCTCCTGGCCCAGGTGAGCAAGGTGGAGGCCTTGCAGTGCCAGGAGGAGGGCTGC
  CGCCATGGGGCAGAGCCCCAGGCGTCCCCAGCTGTTCACCTCCCAGAGAGTCCCCAGAGCCCCAAAGGGC
  TGACTGAGAACTCCAGGCACCGGCTGACCCCTCAGGAGGGATTGCAGGCCCCAGGTGGCAGCCTCAGAGA
  GGAGGCGCAGGCAGGCCCCTGCCCGGCAGCCCCCATCTTCAAGGGCTGCTTCTACACCCACCCCACCGAG
  GTGCTGAAGCCTGTCAGCCAGCACCCCAGGGACTTCTTCTCTAGACTTAAAGATGGGGTGCTATTGGGGC
  CTCCTGGCAAAGAGGGGCTGTCAGTGAAAGAGCCCCAGCTGGTGTGGGGCGGAGACGCTAACCGCCCTTC
  TGCGTTCCATAAAGGTGGCTCCAGAAAGGGCATCCTCTACCCCAAGCCCAAAGCCTGCTGGGTGTCCCCC
  ATGGCCAAGGTCCCAGCCGAGAGCCCCACGCTCCCGCCCACCTTCCCCAGTAGCCCAGGCCTGGGCAGCA
  AGCGCAGCCTGGAGGAAGAGGGTGCTGCCCACAGTGGGAAGAGACTGCGGGCCGTGTCTCCCTTTCTTAA
  GGAGGCGGATGCCAAGAAGTGTGGGGCCAAACCTGCAGGGTCCGGCCTGGTCTCCTGCCTTCTGGGCCCA
  GCCCTGGGGCCTGTGCCCCCAGAGGCCTACAGGGGCACCATGCTGCACTGCCCGCTGAACTTCACTGGCA
  CCCCGGGCCCCTTGAAGGGCCAGGCTGCACTCCCCTTCAGCCCCCTGGTCATCCCGGCCTTCCCGGCCCA
  CTTCCTGGCCACCGCAGGCCCCTCGCCCATGGCCGCTGGCCTGATGCACTTCCCCCCAACGTCCTTCGAC
  AGTGCCCTCCGCCACAGACTTTGCCCGGCCTCATCTGCCTGGCACGCACCACCAGTCACAACCTATGCAG
  CGCCCCACTTCTTCCACCTCAACACCAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216294 representing NM_212481
  Red=Cloning site Green=Tags(s)
  
  MAAPVKGNRKQSTEGDALDPPASPKPAGKQNGIQNPISLEDSPEAGGEREEEQEREEEQAFLVSLYKFMK
  ERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVL
  PYVRHLKGEDDKPLPTSKPRKQYKMAKENRGDDGATERPKKAKEERRMDQMMPGKTKADAADPAPLPSQE
  PPRNSTEQQGLASGSSVSFVGASGCPEAYKRLLSSFYCKGTHGIMSPLAKKKLLAQVSKVEALQCQEEGC
  RHGAEPQASPAVHLPESPQSPKGLTENSRHRLTPQEGLQAPGGSLREEAQAGPCPAAPIFKGCFYTHPTE
  VLKPVSQHPRDFFSRLKDGVLLGPPGKEGLSVKEPQLVWGGDANRPSAFHKGGSRKGILYPKPKACWVSP
  MAKVPAESPTLPPTFPSSPGLGSKRSLEEEGAAHSGKRLRAVSPFLKEADAKKCGAKPAGSGLVSCLLGP
  ALGPVPPEAYRGTMLHCPLNFTGTPGPLKGQAALPFSPLVIPAFPAHFLATAGPSPMAAGLMHFPPTSFD
  SALRHRLCPASSAWHAPPVTTYAAPHFFHLNTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212481
• ORF Size: 1782 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_212481.1, NM_212481.2, NP_997646.1
• RefSeq Size: 2223
• RefSeq ORF: 1785
• Locus ID: 10865
• MW: 63.9 kDa
• Gene Summary: Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]). [supplied by OMIM, Mar 2008]