MAP3K12 (NM_006301) Human Tagged ORF Clone

CAT#: RC216463

MAP3K12 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP3K12
• Synonyms: DLK; MEKK12; MUK; ZPK; ZPKP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216463 representing NM_006301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGCCTCCATGAGACCCGAACACCCTCTCCTTCCTTTGGGGGCTTTGTGTCTACCCTAAGTGAGG
  CATCCATGCGCAAGCTGGACCCAGACACTTCTGACTGCACTCCCGAGAAGGACCTGACGCCTACCCATGT
  CCTGCAGCTACATGAGCAGGATGCAGGGGGCCCAGGGGGAGCAGCTGGGTCACCTGAGAGTCGGGCATCC
  AGAGTTCGAGCTGACGAGGTGCGACTGCAGTGCCAGAGTGGCAGTGGCTTCCTTGAGGGCCTCTTTGGCT
  GCCTGCGCCCTGTCTGGACCATGATTGGCAAAGCCTACTCCACTGAGCACAAGCAGCAGCAGGAAGACCT
  TTGGGAGGTCCCCTTTGAGGAAATCCTGGACCTGCAGTGGGTGGGCTCAGGGGCCCAGGGTGCTGTCTTC
  CTGGGGCGCTTCCACGGGGAGGAGGTGGCTGTGAAGAAGGTGCGAGACCTCAAAGAAACCGACATCAAGC
  ACTTGCGAAAGCTGAAGCACCCCAACATCATCACTTTCAAGGGTGTGTGCACCCAGGCTCCCTGCTACTG
  CATCCTCATGGAGTTCTGCGCCCAGGGCCAGCTGTATGAGGTACTGCGGGCTGGCCGCCCTGTCACCCCC
  TCCTTACTGGTTGACTGGTCCATGGGCATCGCTGGTGGCATGAACTACCTGCACCTGCACAAGATTATCC
  ACAGGGATCTCAAGTCACCCAACATGCTAATCACCTACGACGATGTGGTGAAGATCTCAGATTTTGGCAC
  TTCCAAGGAGCTGAGTGACAAGAGCACCAAGATGTCCTTTGCAGGGACAGTAGCCTGGATGGCCCCTGAG
  GTGATCCGCAATGAACCTGTGTCTGAGAAGGTCGACATCTGGTCCTTTGGCGTGGTGCTATGGGAACTGC
  TGACTGGTGAGATCCCCTACAAAGACGTAGATTCCTCAGCCATTATCTGGGGTGTGGGAAGCAACAGTCT
  CCATCTGCCCGTGCCCTCCAGTTGCCCAGATGGTTTCAAGATCCTGCTTCGCCAGTGCTGGAATAGCAAA
  CCACGAAATCGCCCATCATTCCGACAGATCCTGCTGCATCTGGACATTGCCTCAGCTGATGTACTCTCCA
  CACCCCAGGAGACTTACTTTAAGTCCCAGGCAGAGTGGCGGGAAGAAGTAAAACTGCACTTTGAAAAGAT
  TAAGTCAGAAGGGACCTGTCTGCACCGCCTAGAAGAGGAACTGGTGATGAGGAGGAGGGAGGAGCTCAGA
  CACGCCCTGGACATCAGGGAGCACTATGAAAGGAAGCTGGAGAGAGCCAACAACCTGTATATGGAACTTA
  ATGCCCTCATGTTGCAGCTGGAACTCAAGGAGAGGGAGCTGCTCAGGCGAGAGCAAGCTTTAGAGCGGAG
  GTGCCCAGGCCTGCTGAAGCCACACCCTTCCCGGGGCCTCCTGCATGGAAACACAATGGAGAAGCTTATC
  AAGAAGAGGAATGTGCCACAGAAGCTGTCACCCCATAGCAAAAGGCCAGATATCCTCAAGACGGAGTCTT
  TGCTCCCTAAACTAGATGCAGCCCTGAGTGGGGTGGGGCTTCCTGGGTGTCCTAAGGGCCCCCCCTCACC
  AGGACGGAGTCGCCGTGGCAAGACCCGTCACCGCAAGGCCAGCGCCAAGGGGAGCTGTGGGGACCTGCCT
  GGGCTTCGTACAGCTGTGCCACCCCATGAACCTGGAGGACCAGGAAGCCCAGGGGGCCTAGGAGGGGGAC
  CCTCAGCCTGGGAGGCCTGCCCTCCCGCCCTCCGTGGGCTTCATCATGACCTCCTGCTCCGCAAAATGTC
  TTCATCGTCCCCAGACCTGCTGTCAGCAGCACTAGGGTCCCGGGGCCGGGGGGCCACAGGCGGAGCTGGG
  GATCCTGGCTCACCACCTCCGGCCCGGGGTGACACCCCACCAAGTGAGGGCTCAGCCCCTGGCTCCACCA
  GCCCAGATTCACCTGGGGGAGCCAAAGGGGAACCACCTCCTCCAGTAGGGCCTGGTGAAGGTGTGGGGCT
  TCTGGGAACTGGAAGGGAAGGGACCTCAGGCCGGGGAGGAAGCCGGGCTGGGTCCCAGCACTTGACCCCA
  GCTGCACTGCTGTACAGGGCTGCCGTCACCCGAAGTCAGAAACGTGGCATCTCATCGGAAGAGGAGGAAG
  GAGAGGTAGACAGTGAAGTAGAGCTGACATCAAGCCAGAGGTGGCCTCAGAGCCTGAACATGCGCCAGTC
  ACTATCTACCTTCAGCTCAGAGAATCCATCAGATGGGGAGGAAGGCACAGCTAGTGAACCTTCCCCCAGT
  GGCACACCTGAAGTTGGCAGCACCAACACTGATGAGCGGCCAGATGAGCGGTCTGATGACATGTGCTCCC
  AGGGCTCAGAAATCCCACTGGACCCACCTCCTTCAGAGGTCATCCCTGGCCCTGAACCCAGCTCCCTGCC
  CATTCCACACCAGGAACTTCTCAGAGAGCGGGGCCCTCCCAATTCTGAGGACTCAGACTGTGACAGCACT
  GAATTGGACAACTCCAACAGCGTTGATGCCTTGCGGCCCCCAGCTTCCCTCCCTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216463 representing NM_006301
  Red=Cloning site Green=Tags(s)
  
  MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGAAGSPESRAS
  RVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVF
  LGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTP
  SLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPE
  VIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSK
  PRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELR
  HALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGLLHGNTMEKLI
  KKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLP
  GLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGSRGRGATGGAG
  DPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTP
  AALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQSLNMRQSLSTFSSENPSDGEEGTASEPSPS
  GTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEVIPGPEPSSLPIPHQELLRERGPPNSEDSDCDST
  ELDNSNSVDALRPPASLPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006301
• ORF Size: 2577 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006301.1, NM_006301.2, NM_006301.3, NP_006292.2, NP_006292.3
• RefSeq Size: 3541
• RefSeq ORF: 2580
• Locus ID: 7786
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• MW: 93.7 kDa
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2010]