CABYR (NM_153768) Human Tagged ORF Clone

CAT#: RC216504

CABYR (Myc-DDK-tagged)-Human calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406955) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216504 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CABYR
• Synonyms: CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86; CT88; FSP-2; FSP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216504 representing NM_153768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCTTCAAAGCCCAGACTTGTCGTACCCTATGGCCTCAAGACTCTGCTCGAGGGAATTAGCAGAG
  CTGTTCTCAAAACCAACCCATCAAACATCAACCAGTTTGCAGCAGCTTATTTTCAAGAACTTACTATGTA
  TAGAGTAGAGAAATGGTCAGAAGGAACGACACCACAGAAGAAATTAGAATGTTTAAAAGAACCAGGAAAA
  ACATCTGTAGAATCTAAAGTACCTACCCAGATGGAAAAATCTACAGACACAGACGAGGACAATGTAACCA
  GAACAGAATATAGTGACAAAACCACCCAGTTTCCATCAGTTTATGCTGTGCCAGGCACTGAGCAAACGGA
  AGCAGTTGGTGGTCTTTCTTCCAAACCAGCCACCCCTAAGACTACTACCCCACCCTCATCACCACCTCCA
  ACAGCTGTCTCACCAGAGTTTGCCTACGTCCCAGCTGACCCAGCTCAGCTTGCTGCTCAGATGTTAGGTA
  AAGTTTCATCTATTCATTCTGATCAATCTGATGTGTTAATGGTGGATGTGGCAACCAGTATGCCTGTTGT
  TATCAAGGAGGTGCCAAGCTCAGAGGCTGCTGAAGATGTCATGGTGGCTGCTCCTCTTGTGTGTTCTGGA
  AAGGTGCTAGAAGTGCAGGTTGTGAACCAAACATCTGTCCATGTAGATTTGGGTTCTCAACCTAAAGAAA
  ATGAGGCTGAACCATCAACGGCTTCCTCAGTCCCCTTGCAGGATGAACAAGAACCTCCTGCTTATGATCA
  AGCTCCTGAGGTCACTTTGCAGGCTGATATTGAGGTTATGTCAACTGTTCATATATCATCTGTCTATAAC
  GATGTGCCTGTGACTGAAGGAGTTGTTTATATCGAGCAACTGCCAGAACAAATAGTTATCCCTTTTACTG
  ATCAAGTTGCTTGTCTTAAAGAAAATGAGCAGTCAAAAGAAAATGAGCAGTCACCACGAGTTAGTCCCAA
  ATCTGTAGTAGAAAAGACCACCTCTGGCATGTCTAAAAAATCTGTAGAGTCTGTAAAACTTGCACAGTTG
  GAGGAGAATGCAAAATATTCCTCAGTATATATGGAGGCAGAAGCAACAGCTCTGCTCTCTGACACATCTT
  TGAAAGGTCAGCCTGAGGTACCTGCACAACTCCTGGATGCAGAAGGTGCTATCAAAATAGGCTCTGAAAA
  ATCTCTGCACCTTGAAGTGGAGATCACTTCAATAGTCTCTGACAATACTGGGCAGGAGGAGTCTGGGGAA
  AACTCTGTACCCCAGGAGATGGAAGGCAAACCTGTGCTCTCTGGGGAAGCTGCAGAAGCAGTGCACTCAG
  GTACATCTGTAAAGTCATCTAGTGGCCCCTTCCCTCCTGCTCCAGAAGGCCTTACTGCACCAGAAATTGA
  ACCAGAAGGGGAATCAACAGCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216504 representing NM_153768
  Red=Cloning site Green=Tags(s)
  
  MISSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRVEKWSEGTTPQKKLECLKEPGK
  TSVESKVPTQMEKSTDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGLSSKPATPKTTTPPSSPPP
  TAVSPEFAYVPADPAQLAAQMLGKVSSIHSDQSDVLMVDVATSMPVVIKEVPSSEAAEDVMVAAPLVCSG
  KVLEVQVVNQTSVHVDLGSQPKENEAEPSTASSVPLQDEQEPPAYDQAPEVTLQADIEVMSTVHISSVYN
  DVPVTEGVVYIEQLPEQIVIPFTDQVACLKENEQSKENEQSPRVSPKSVVEKTTSGMSKKSVESVKLAQL
  EENAKYSSVYMEAEATALLSDTSLKGQPEVPAQLLDAEGAIKIGSEKSLHLEVEITSIVSDNTGQEESGE
  NSVPQEMEGKPVLSGEAAEAVHSGTSVKSSSGPFPPAPEGLTAPEIEPEGESTAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153768
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153768.1, NM_153768.2, NP_722452.1
• RefSeq Size: 2279
• RefSeq ORF: 1428
• Locus ID: 26256
• MW: 50.5 kDa
• Gene Summary: To reach fertilization competence, spermatozoa undergo a series of morphological and molecular maturational processes, termed capacitation, involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2013]