SPATIAL (TBATA) (NM_152710) Human Tagged ORF Clone

CAT#: RC216613

TBATA (Myc-DDK-tagged)-Human chromosome 10 open reading frame 27 (C10orf27)

Product Images

Western blot validation of overexpression lysate (Cat# LY407352) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216613 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBATA
• Synonyms: C10orf27; SPATIAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216613 representing NM_152710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGATGTTCAATTGGCTGGTTATCCACTGATGAGTCCAAAGGCTGAGCTGAAACTGGAGAAGA
  AGTCAGGGCGCAAGCCAAGGAGCCCCAGGGACAGTGGGCCACAGAAAGAACTGGTGATCCCAGGGATTGT
  GGATTTCGAGCGGATCCGCCGGGCATTGAGGACCCCAAAGCCCCAAACCCCTGGCACCTACTGCTTTGGA
  CGCCTCAGTCACCACTCCTTCTTCTCCCGGCACCACCCACACCCCCAGCACGTGACCCACATCCAAGATC
  TCACCGGGAAGCCTGTCTGTGTCGTCAGGGATTTTCCAGCCCCCTTGCCTGAGTCAACTGTCTTTTCCGG
  CTGTCAAATGGGGATACCCACCATCTCTGTCCCCATTGGAGACCCACAGTCTAATCGGAACCCCCAGCTT
  TCTTCTGAAGCCTGGAAGAAGGAGTTGAAGGAGCTAGCTTCCCGGGTGGCCTTCCTCACCAAGGAGGATG
  AACTGAAGAAGAAAGAGAAGGAGCAGAAGGAGGAGCCTCTGCGGGAGCAGGGGGCAAAGTACTCAGCAGA
  GACTGGGAGGCTCATCCCCGCTTCCACCCGGGCTGTCGGCCGCCGCAGATCTCACCAGGGCCAGCAGAGT
  CAGTCTTCCAGCAGACATGAAGGAGTCCAGGCCTTCCTCCTTCAGGATCAGGAGCTGCTGGTCCTGGAGC
  TCCTGTGTCAGATCCTGGAAACAGACTTGCTAAGCGCAATCCAGTTCTGGCTGCTCTACGCTCCGCCCAA
  GGAAAAAGACCTCGCTCTGGGACTCCTGCAGACAGCAGTGGCTCAGCTCCTTCCCCAGCCCCTAGTCTCC
  ATCCCTACAGAAAAGCTCCTAAGCCAGCTTCCAGAAGTGCATGAACCTCCTCAAGAGAAGCAAGAGCCAC
  CCTGCAGTCAATCCCCGAAGAAAACGAAGATATCACCTTTTACAAAAAGCGAAAAACCAGAGTACATTGG
  AGAAGCTCAAGTCCTCCAGATGCATTCAAGCCAGAACACAGAGAAGAAGACATCGAAGCCGAGGGCAGAG
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216613 representing NM_152710
  Red=Cloning site Green=Tags(s)
  
  MATDVQLAGYPLMSPKAELKLEKKSGRKPRSPRDSGPQKELVIPGIVDFERIRRALRTPKPQTPGTYCFG
  RLSHHSFFSRHHPHPQHVTHIQDLTGKPVCVVRDFPAPLPESTVFSGCQMGIPTISVPIGDPQSNRNPQL
  SSEAWKKELKELASRVAFLTKEDELKKKEKEQKEEPLREQGAKYSAETGRLIPASTRAVGRRRSHQGQQS
  QSSSRHEGVQAFLLQDQELLVLELLCQILETDLLSAIQFWLLYAPPKEKDLALGLLQTAVAQLLPQPLVS
  IPTEKLLSQLPEVHEPPQEKQEPPCSQSPKKTKISPFTKSEKPEYIGEAQVLQMHSSQNTEKKTSKPRAE
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152710
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152710.1, NM_152710.2, NM_152710.3, NP_689923.1
• RefSeq Size: 1583 bp
• RefSeq ORF: 1056 bp
• Locus ID: 219793
• MW: 39.3 kDa
• Gene Summary: This gene encodes a protein that regulates thymic epithelial cell proliferation and thymus size. It has been identified as a ligand for the class I human leukocyte antigen (HLA-I) in thymus. Studies of the orthologous mouse protein suggest that it may also play a role in spermatid differentiation, as well as in neuronal morphogenesis and synaptic plasticity. Polymorphisms in this gene are associated with susceptibility for multiple sclerosis (MS). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]