ANKK1 (NM_178510) Human Tagged ORF Clone

CAT#: RC216746

ANKK1 (Myc-DDK-tagged)-Human ankyrin repeat and kinase domain containing 1 (ANKK1)

Product Images

Western blot validation of overexpression lysate (Cat# LY405898) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216746 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKK1
• Synonyms: PKK2; sgK288
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216746 representing NM_178510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGACCCCACCGAGCTGCGGCTGGGCAGCCTCCCCGTCTTCACCCGCGACGACTTCGAGGGCG
  ACTGGCGCCTAGTGGCCAGCGGCGGCTTCAGCCAGGTGTTCCAGGCGCGGCACAGGCGCTGGCGGACGGA
  GTACGCCATCAAGTGCGCCCCCTGCCTTCCACCCGACGCCGCCAGCTCTGATGTGAATTACCTCATTGAA
  GAAGCTGCCAAAATGAAGAAGATCAAGTTTCAGCACATCGTGTCTATCTACGGGGTGTGCAAGCAGCCCC
  TGGGTATTGTGATGGAGTTTATGGCCAACGGCTCCCTGGAGAAGGTGCTGTCCACCCACAGCCTCTGCTG
  GAAGCTCAGGTTCCGCATCATCCATGAGACCAGCTTGGCCATGAACTTCCTGCACAGCATTAAGCCGCCT
  CTGCTCCACCTGGACCTCAAGCCGGGCAACATACTCCTGGACAGCAACATGCATGTCAAAATTTCAGACT
  TCGGCCTGTCCAAGTGGATGGAACAGTCCACCCGGATGCAGTACATCGAGAGGTCGGCTCTGCGGGGCAT
  GCTCAGCTACATCCCCCCTGAGATGTTCCTGGAGAGTAACAAGGCCCCAGGACCTAAATATGATGTGTAC
  AGCTTTGCAATTGTCATCTGGGAGCTACTCACTCAGAAGAAACCATACTCAGGGTTCAACATGATGATGA
  TTATTATCCGAGTGGCGGCAGGCATGCGGCCCTCCCTACAGCCTGTCTCTGACCAATGGCCAAGCGAGGC
  CCAGCAGATGGTGGACCTGATGAAACGCTGCTGGGACCAGGACCCCAAGAAGAGGCCATGCTTTCTAGAC
  ATTACCATCGAGACAGACATACTGCTGTCACTGCTGCAGAGTCGTGTGGCAGTCCCAGAGAGCAAGGCCC
  TGGCCAGGAAGGTGTCCTGCAAGCTGTCGCTGCGCCAGCCCGGGGAGGTTAATGAGGACATCAGCCAGGA
  ACTGATGGACAGTGACTCAGGAAACTACCTGAAGCGGGCCCTTCAGCTCTCCGACCGTAAGAATTTGGTC
  CCGAGAGATGAGGAACTGTGTATCTATGAGAACAAGGTCACCCCCCTCCACTTCCTGGTGGCCCAGGGCA
  GTGTGGAGCAGGTGAGGTTGCTGCTGGCCCACGAGGTAGACGTGGACTGCCAGACGGCCTCTGGATACAC
  GCCCCTCCTGATCGCCGCCCAGGACCAGCAACCCGACCTCTGTGCCCTGCTTTTGGCACATGGTGCTGAT
  GCCAACCGAGTGGATGAGGATGGCTGGGCCCCACTGCACTTTGCAGCCCAGAATGGGGATGACGGCACTG
  CGCGCCTGCTCCTGGACCACGGGGCCTGTGTGGATGCCCAGGAACGTGAAGGGTGGACCCCTCTTCACCT
  GGCTGCACAGAATAACTTTGAGAATGTGGCACGGCTTCTGGTCTCCCGTCAGGCTGACCCCAACCTGCAT
  GAGGCTGAGGGCAAGACCCCCCTCCATGTGGCCGCCTACTTTGGCCATGTTAGCCTGGTCAAGCTGCTGA
  CCAGCCAGGGGGCTGAGTTGGATGCTCAGCAGAGAAACCTGAGAACACCACTGCACCTGGCAGTAGAGCG
  GGGCAAAGTGAGGGCCATCCAACACCTGCTGAAGAGTGGAGCGGTCCCTGATGCCCTTGACCAGAGCGGC
  TACGGCCCACTGCACACTGCAGCTGCCAGGGGCAAATACCTGATCTGCAAGATGCTGCTCAGGTACGGAG
  CCAGCCTTGAGCTGCCCACCCACCAGGGCTGGACACCCCTGCATCTAGCAGCCTACAAGGGCCACCTGGA
  GATCATCCATCTGCTGGCAGAGAGCCACGCAAACATGGGTGCTCTTGGAGCTGTGAACTGGACTCCCCTG
  CACCTAGCTGCACGCCACGGGGAGGAGGCGGTGGTGTCAGCACTGCTGCAGTGTGGGGCTGACCCCAATG
  CTGCAGAGCAGTCAGGCTGGACACCCCTCCACCTGGCGGTCCAGAGGAGCACCTTCCTGAGTGTCATCAA
  CCTCCTAGAACATCACGCAAATGTCCACGCCCGCAACAAGGTGGGCTGGACACCCGCCCACCTGGCCGCC
  CTCAAGGGCAACACAGCCATCCTCAAAGTGCTGGTCGAGGCAGGCGCCCAGCTGGACGTCCAGGATGGAG
  TGAGCTGCACACCCCTGCAACTGGCCCTCCGCAGCCGAAAGCAGGGCATCATGTCCTTCCTAGAGGGCAA
  GGAGCCGTCAGTGGCCACTCTGGGTGGTTCTAAGCCAGGAGCCGAGATGGAAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216746 representing NM_178510
  Red=Cloning site Green=Tags(s)
  
  MAADPTELRLGSLPVFTRDDFEGDWRLVASGGFSQVFQARHRRWRTEYAIKCAPCLPPDAASSDVNYLIE
  EAAKMKKIKFQHIVSIYGVCKQPLGIVMEFMANGSLEKVLSTHSLCWKLRFRIIHETSLAMNFLHSIKPP
  LLHLDLKPGNILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGMLSYIPPEMFLESNKAPGPKYDVY
  SFAIVIWELLTQKKPYSGFNMMMIIIRVAAGMRPSLQPVSDQWPSEAQQMVDLMKRCWDQDPKKRPCFLD
  ITIETDILLSLLQSRVAVPESKALARKVSCKLSLRQPGEVNEDISQELMDSDSGNYLKRALQLSDRKNLV
  PRDEELCIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTPLLIAAQDQQPDLCALLLAHGAD
  ANRVDEDGWAPLHFAAQNGDDGTARLLLDHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQADPNLH
  EAEGKTPLHVAAYFGHVSLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLKSGAVPDALDQSG
  YGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLHLAAYKGHLEIIHLLAESHANMGALGAVNWTPL
  HLAARHGEEAVVSALLQCGADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHLAA
  LKGNTAILKVLVEAGAQLDVQDGVSCTPLQLALRSRKQGIMSFLEGKEPSVATLGGSKPGAEMEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178510
• ORF Size: 2295 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178510.1, NP_848605.1
• RefSeq Size: 2543
• RefSeq ORF: 2298
• Locus ID: 255239
• Protein Families: Druggable Genome, Protein Kinase
• MW: 84.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11, and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA, was originally associated with the DRD2 gene, however, later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654, 15146457), where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP. [provided by RefSeq, Sep 2009]