PAX7 (NM_013945) Human Tagged ORF Clone

CAT#: RC216836

PAX7 (Myc-DDK-tagged)-Human paired box 7 (PAX7), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY415548) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216836 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAX7
• Synonyms: HUP1; PAX7B; RMS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216836 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTTCCCGGCACGGTACCGAGAATGATGCGGCCGGCTCCGGGGCAGAACTACCCCCGCACGG
  GATTCCCTTTGGAAGTGTCCACCCCGCTTGGCCAAGGCCGGGTCAATCAGCTGGGAGGGGTCTTCATCAA
  TGGGCGACCCCTGCCTAACCACATCCGCCACAAGATAGTGGAGATGGCCCACCATGGCATCCGGCCCTGT
  GTCATCTCCCGACAGCTGCGTGTCTCCCACGGCTGCGTCTCCAAGATTCTTTGCCGCTACCAGGAGACCG
  GGTCCATCCGGCCTGGGGCCATCGGCGGCAGCAAGCCCAGACAGGTGGCGACTCCGGATGTAGAGAAAAA
  GATTGAGGAGTACAAGAGGGAAAACCCAGGCATGTTCAGCTGGGAGATCCGGGACAGGCTGCTGAAGGAT
  GGGCACTGTGACCGAAGCACTGTGCCCTCAGTGAGTTCGATTAGCCGCGTGCTCAGAATCAAGTTCGGGA
  AGAAAGAGGAGGAGGATGAAGCGGACAAGAAGGAGGACGACGGCGAAAAGAAGGCCAAACACAGCATCGA
  CGGCATCCTGGGCGACAAAGGGAACCGGCTGGACGAGGGCTCGGATGTGGAGTCGGAACCTGACCTCCCA
  CTGAAGCGCAAGCAGCGACGCAGTCGGACCACATTCACGGCCGAGCAGCTGGAGGAGCTGGAGAAGGCCT
  TTGAGAGGACCCACTACCCAGACATATACACCCGCGAGGAGCTGGCGCAGAGGACCAAGCTGACAGAGGC
  GCGTGTGCAGGTCTGGTTCAGTAACCGCCGCGCCCGTTGGCGTAAGCAGGCAGGAGCCAACCAGCTGGCG
  GCGTTCAACCACCTTCTGCCAGGAGGCTTCCCGCCCACCGGCATGCCCACGCTGCCCCCCTACCAGCTGC
  CGGACTCCACCTACCCCACCACCACCATCTCCCAAGATGGGGGCAGCACTGTGCACCGGCCTCAGCCCCT
  GCCACCGTCCACCATGCACCAGGGCGGGCTGGCTGCAGCGGCTGCAGCCGCCGACACCAGCTCTGCCTAC
  GGAGCCCGCCACAGCTTCTCCAGCTACTCTGACAGCTTCATGAATCCGGCGGCGCCCTCCAACCACATGA
  ACCCGGTCAGCAACGGCCTGTCTCCTCAGGTGATGAGCATCTTGGGCAACCCCAGTGCGGTGCCCCCGCA
  GCCACAGGCTGACTTCTCCATCTCCCCGCTGCATGGCGGCCTGGACTCGGCCACCTCCATCTCAGCCAGC
  TGCAGCCAGCGGGCCGACTCCATCAAGCCAGGAGACAGCCTGCCCACCTCCCAGGCCTACTGCCCACCCA
  CCTACAGCACCACCGGCTACAGCGTGGACCCCGTGGCCGGCTATCAGTACGGCCAGTACGGCCAGAGTGA
  GTGCCTGGTGCCCTGGGCGTCCCCCGTCCCCATTCCTTCTCCCACCCCCAGGGCCTCCTGCTTGTTTATG
  GAGAGCTACAAGGTGGTGTCAGGGTGGGGAATGTCCATTTCACAGATGGAAAAATTGAAGTCCAGCCAGA
  TGGAACAGTTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216836 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPC
  VISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKD
  GHCDRSTVPSVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLP
  LKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLA
  AFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAY
  GARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISAS
  CSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFM
  ESYKVVSGWGMSISQMEKLKSSQMEQFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013945
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013945.1, NM_013945.2, NP_039236.1
• RefSeq Size: 2265 bp
• RefSeq ORF: 1557 bp
• Locus ID: 5081
• Cytogenetics: 1p36.13
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• MW: 56.7 kDa
• Gene Summary: 'This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]'