SIN3B (NM_015260) Human Tagged ORF Clone

CAT#: RC216859

SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B)

Product Images

Western blot validation of overexpression lysate (Cat# LY414651) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216859 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIN3B
• Synonyms: KIAA0700
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216859 representing NM_015260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCACGCTGGCGGTGGCAGCGGTGGCAGCGGTGCCGGCGGCCCCGCGGGCCGGGGGCTGAGCGGCG
  CCCGCTGGGGTCGCTCGGGCTCCGCAGGCCACGAGAAGCTGCCGGTGCACGTAGAAGACGCCCTCACCTA
  TCTGGACCAGGTGAAGATCCGCTTTGGCAGCGACCCTGCCACCTACAACGGCTTCCTGGAGATCATGAAG
  GAGTTCAAAAGCCAGAGCATCGATACTCCTGGAGTCATCAGACGTGTCTCGCAGCTCTTCCACGAGCACC
  CTGACCTCATTGTTGGATTCAACGCTTTTCTTCCCCTCGGATATAGAATAGACATTCCCAAGAATGGCAA
  GTTAAACATACAGTCGCCTCTGACAAGCCAGGAGAATTCGCACAACCACGGGGACGGTGCAGAGGACTTC
  AAGCAGCAGGTGCCGTATAAAGAGGACAAACCCCAGGTGCCCCTGGAGTCCGATTCCGTGGAATTCAACA
  ACGCCATCAGCTATGTGAATAAGATTAAAACCCGCTTCCTAGACCACCCAGAAATCTACAGGTCATTCCT
  GGAGATCCTGCACACGTACCAGAAGGAGCAGCTGAACACGAGGGGCCGGCCATTCCGAGGCATGTCTGAA
  GAGGAGGTGTTCACCGAGGTGGCCAACCTCTTCCGGGGCCAGGAGGACCTGCTCTCAGAGTTTGGACAGT
  TCCTGCCCGAAGCCAAGCGGTCTCTGTTCACAGGAAACGGGCCGTGCGAGATGCACAGCGTGCAGAAGAA
  CGAGCACGACAAGACCCCGGAGCACAGCAGGAAGCGCTCCCGGCCCTCGCTCCTCCGCCCCGTGTCTGCA
  CCCGCCAAGAAAAAAATGAAACTTCGTGGTACCAAAGACCTGTCCATCGCTGCAGTGGGGAAGTACGGGA
  CTCTGCAGGAATTTTCTTTCTTTGACAAGGTCCGCCGGGTGCTGAAGAGCCAGGAGGTGTATGAAAACTT
  CCTCCGCTGCATCGCACTCTTCAACCAGGAGCTGGTGTCTGGCTCTGAGCTCCTGCAGCTCGTCAGCCCA
  TTTCTGGGGAAATTTCCAGAACTCTTTGCACAGTTCAAGTCCTTCCTGGGGGTAAAAGAGCTGTCCTTCG
  CGCCACCCATGAGCGACAGATCCGGGGACGGGATAAGCCGGGAAATTGATTATGCATCCTGCAAGCGCAT
  AGGATCCAGCTACCGGGCACTCCCCAAAACCTACCAGCAGCCCAAGTGCAGTGGGAGGACAGCCATCTGC
  AAGGAGCTTGACCATTGGACACTTCTCCAGGGTTCGTGGACAGACGATTACTGCATGTCCAAGTTCAAGA
  ATACCTGCTGGATTCCAGGATATAGTGCAGGGGTACTGAACGACACCTGGGTCTCCTTCCCTTCCTGGTC
  TGAGGACTCCACGTTCGTCAGCTCCAAGAAGACACCGTACGAGGAGCAGCTTCACCGCTGTGAGGACGAG
  CGCTTCGAGTTAGACGTTGTCCTGGAGACGAACCTGGCCACAATCCGTGTGTTGGAAAGTGTGCAGAAGA
  AGCTGTCTCGGATGGCGCCGGAAGACCAGGAGAAGTTCCGGCTGGACGACTCCCTGGGAGGCACGTCGGA
  GGTGATCCAGCGCCGTGCCATTTATCGCATCTATGGCGACAAGGCCCCGGAGATCATCGAGAGCCTCAAG
  AAGAACCCTGTCACCGCTGTCCCCGTTGTCCTGAAAAGACTGAAGGCCAAGGAAGAGGAGTGGCGGGAGG
  CCCAGCAGGGCTTCAACAAGATCTGGCGGGAGCAGTATGAGAAGGCGTACCTCAAGTCCCTTGACCACCA
  GGCTGTGAACTTCAAGCAGAACGACACCAAGGCCCTGCGCTCCAAGAGCTTGCTCAACGAGATCGAGAGC
  GTCTACGACGAGCACCAGGAGCAGCACTCGGAGGGCCGCAGTGCCCCCTCTAGCGAGCCGCACCTCATCT
  TTGTGTACGAGGACCGGCAGATCCTGGAGGACGCAGCAGCGCTCATCAGCTACTACGTGAAGCGGCAGCC
  GGCCATCCAGAAGGAGGACCAGGGCACCATCCACCAGCTGCTGCACCAGTTCGTGCCCAGCCTCTTCTTC
  TCTCAGCAGCTGGACCTGGGCGCCTCCGAGGAGTCAGCTGATGAGGACCGGGACAGCCCCCAGGGGCAGA
  CCACAGACCCCAGTGAGCGGAAGAAGCCGGCGCCAGGACCCCACAGTAGCCCCCCAGAGGAGAAGGGGGC
  CTTCGGGGATGCCCCGGCCACTGAGCAGCCACCCCTGCCGCCCCCAGCCCCGCACAAGCCCCTGGACGAT
  GTCTACAGCCTATTTTTTGCCAACAACAACTGGTACTTCTTCCTGCGCCTGCACCAGACCCTGTGCTCCA
  GGCTGCTGAAGATCTACCGCCAGGCGCAGAAGCAGCTTCTGGAGTATCGGACCGAGAAGGAGCGGGAGAA
  GCTGCTGTGTGAGGGCCGCAGGGAGAAGGGCAGCGACCCCGCCATGGAGCTGCGGCTGAAGCAGCCCAGT
  GAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGAGCCTGCTGGAGGGCAGCATCG
  ACCCCACGCAGTACGAGGACACCCTACGCGAGATGTTCACCATCCATGCCTACGTGGGCTTCACCATGGA
  CAAGCTGGTGCAGAACATTGCGCGGCAGCTGCACCACCTCGTGAGCGATGACGTCTGCCTGAAAGTGGTG
  GAGCTCTACCTGAACGAGAAGAAGCGGGGTGCCGCTGGTGGGAACCTGTCCTCCCGCTGCGTCCGCGCTG
  CTAGGGAGACCAGCTACCAGTGGAAGGCTGAGCGCTGCATGGCCGACGAGAACTGCTTCAAGGTGATGTT
  CCTGCAGCGCAAAGGGCAGGTGATCATGACCATCGAGCTCCTGGACACCGAGGAGGCCCAGACGGAGGAC
  CCTGTGGAGGTCCAGCACCTGGCTCGGTACGTGGAGCAGTATGTGGGGACCGAGGGCGCGTCCAGCTCGC
  CCACTGAGGGCTTCCTCCTGAAACCTGTGTTCCTGCAGAGGAACCTCAAGAAGTTCCGCCGCCGGTGGCA
  GAGCGAGCAGGCGCGGGCCCTGCGCGGTGAGGCCAGGAGCTCCTGGAAGCGGCTGGTGGGCGTGGAGAGC
  GCCTGCGACGTGGACTGCCGCTTCAAGCTCAGCACTCACAAGATGGTGTTCATCGTGAACTCCGAGGACT
  ACATGTACCGTCGCGGGACCCTCTGCCGGGCCAAGCAGGTGCAGCCCCTGGTCCTGCTCCGCCACCACCA
  GCACTTTGAGGAGTGGCACAGCCGCTGGCTGGAGGACAATGTGACGGTGGAGGCGGCTAGCCTGGTGCAG
  GACTGGCTGATGGGTGAGGAGGACGAGGACATGGTACCCTGCAAGACGCTGTGTGAGACAGTGCACGTGC
  ACGGCCTGCCCGTGACCCGCTACCGCGTGCAGTACAGCCGCCGCCCGGCCTCGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216859 representing NM_015260
  Red=Cloning site Green=Tags(s)
  
  MAHAGGGSGGSGAGGPAGRGLSGARWGRSGSAGHEKLPVHVEDALTYLDQVKIRFGSDPATYNGFLEIMK
  EFKSQSIDTPGVIRRVSQLFHEHPDLIVGFNAFLPLGYRIDIPKNGKLNIQSPLTSQENSHNHGDGAEDF
  KQQVPYKEDKPQVPLESDSVEFNNAISYVNKIKTRFLDHPEIYRSFLEILHTYQKEQLNTRGRPFRGMSE
  EEVFTEVANLFRGQEDLLSEFGQFLPEAKRSLFTGNGPCEMHSVQKNEHDKTPEHSRKRSRPSLLRPVSA
  PAKKKMKLRGTKDLSIAAVGKYGTLQEFSFFDKVRRVLKSQEVYENFLRCIALFNQELVSGSELLQLVSP
  FLGKFPELFAQFKSFLGVKELSFAPPMSDRSGDGISREIDYASCKRIGSSYRALPKTYQQPKCSGRTAIC
  KELDHWTLLQGSWTDDYCMSKFKNTCWIPGYSAGVLNDTWVSFPSWSEDSTFVSSKKTPYEEQLHRCEDE
  RFELDVVLETNLATIRVLESVQKKLSRMAPEDQEKFRLDDSLGGTSEVIQRRAIYRIYGDKAPEIIESLK
  KNPVTAVPVVLKRLKAKEEEWREAQQGFNKIWREQYEKAYLKSLDHQAVNFKQNDTKALRSKSLLNEIES
  VYDEHQEQHSEGRSAPSSEPHLIFVYEDRQILEDAAALISYYVKRQPAIQKEDQGTIHQLLHQFVPSLFF
  SQQLDLGASEESADEDRDSPQGQTTDPSERKKPAPGPHSSPPEEKGAFGDAPATEQPPLPPPAPHKPLDD
  VYSLFFANNNWYFFLRLHQTLCSRLLKIYRQAQKQLLEYRTEKEREKLLCEGRREKGSDPAMELRLKQPS
  EVELEEYYPAFLDMVRSLLEGSIDPTQYEDTLREMFTIHAYVGFTMDKLVQNIARQLHHLVSDDVCLKVV
  ELYLNEKKRGAAGGNLSSRCVRAARETSYQWKAERCMADENCFKVMFLQRKGQVIMTIELLDTEEAQTED
  PVEVQHLARYVEQYVGTEGASSSPTEGFLLKPVFLQRNLKKFRRRWQSEQARALRGEARSSWKRLVGVES
  ACDVDCRFKLSTHKMVFIVNSEDYMYRRGTLCRAKQVQPLVLLRHHQHFEEWHSRWLEDNVTVEAASLVQ
  DWLMGEEDEDMVPCKTLCETVHVHGLPVTRYRVQYSRRPASP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015260
• ORF Size: 3486 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015260.1, NM_015260.2, NM_015260.3, NP_056075.1
• RefSeq Size: 5129
• RefSeq ORF: 3489
• Locus ID: 23309
• MW: 132.9 kDa