NUMBL (NM_004756) Human Tagged ORF Clone

CAT#: RC216869

NUMBL (Myc-DDK-tagged)-Human numb homolog (Drosophila)-like (NUMBL)

Product Images

Western blot validation of overexpression lysate (Cat# LY417769) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216869 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NUMBL protein (Cat# TP316869). The protein was produced from HEK293T cells transfected with NUMBL cDNA clone (Cat# RC216869) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUMBL
• Synonyms: CAG3A; CTG3a; NBL; NUMB-R; NUMBLIKE; NUMBR; TNRC23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216869 representing NM_004756
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGCAGCGCGGCGGCCAGCGGCGGACCCCGGAGGCCTGAGCGGCACCTGCCCCCAGCCCCCTGTG
  GGGCCCCGGGGCCCCCAGAAACCTGCAGGACGGAGCCAGACGGGGCGGGCACCATGAACAAGTTACGGCA
  GAGCCTGCGGCGGAGGAAGCCAGCCTACGTGCCCGAGGCGTCGCGCCCGCACCAGTGGCAGGCAGACGAG
  GACGCGGTGCGGAAGGGCACGTGCAGCTTCCCGGTCAGGTACCTGGGTCACGTGGAGGTAGAGGAGTCCC
  GGGGAATGCACGTGTGTGAAGATGCGGTGAAGAAGCTGAAGGCGATGGGCCGAAAGTCCGTGAAGTCTGT
  CCTGTGGGTGTCAGCCGATGGGCTCCGAGTGGTGGACGACAAAACCAAGGATCTTCTGGTCGACCAGACC
  ATCGAAAAGGTCTCCTTTTGTGCTCCTGACCGCAACCTGGACAAGGCTTTCTCCTATATCTGTCGTGACG
  GGACTACCCGCCGCTGGATCTGCCACTGTTTTCTGGCACTGAAGGACTCCGGCGAGAGGCTGAGCCACGC
  TGTGGGCTGTGCTTTTGCCGCCTGCCTGGAGCGAAAACAGCGACGGGAGAAGGAATGTGGGGTCACGGCC
  GCCTTCGATGCCAGCCGCACCAGCTTCGCCCGCGAGGGCTCCTTCCGCCTGTCTGGGGGTGGGCGGCCTG
  CTGAGCGAGAGGCCCCGGACAAGAAGAAAGCAGAGGCAGCAGCTGCCCCCACTGTGGCTCCTGGCCCTGC
  CCAGCCTGGGCACGTGTCCCCGACACCAGCCACCACATCCCCTGGTGAGAAGGGTGAGGCAGGCACCCCT
  GTGGCTGCAGGCACCACTGCGGCCGCCATCCCCCGGCGCCATGCACCCCTGGAGCAGCTGGTTCGCCAGG
  GCTCCTTCCGTGGGTTCCCAGCACTCAGCCAGAAGAACTCGCCTTTCAAACGGCAGCTGAGCCTACGGCT
  GAATGAGCTGCCATCCACGCTGCAGCGCCGCACTGACTTCCAGGTGAAGGGCACAGTGCCTGAGATGGAG
  CCTCCTGGTGCCGGCGACAGTGACAGCATCAACGCTCTGTGCACACAGATCAGTTCATCTTTTGCCAGTG
  CTGGAGCGCCAGCACCAGGGCCACCACCTGCCACAACAGGGACTTCTGCCTGGGGTGAGCCCTCCGTGCC
  CCCTGCAGCTGCCTTCCAGCCTGGGCACAAGCGGACACCTTCAGAGGCTGAGCGATGGCTGGAGGAGGTG
  TCACAGGTGGCCAAGGCCCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAAC
  AGCAGCAAGCAGCCTCAGTGGCCCCAGTGCCCACCATGCCTCCTGCCCTGCAGCCTTTCCCCGCCCCCGT
  GGGGCCCTTTGACGCTGCACCTGCCCAAGTGGCCGTGTTCCTGCCACCCCCACACATGCAGCCCCCTTTT
  GTGCCCGCCTACCCGGGCTTGGGCTACCCACCGATGCCCCGGGTGCCCGTGGTGGGCATCACACCCTCAC
  AGATGGTGGCAAACGCCTTCTGCTCAGCCGCCCAGCTCCAGCCTCAGCCTGCCACTCTGCTTGGGAAAGC
  TGGGGCCTTCCCGCCCCCTGCCATACCCAGTGCCCCTGGGAGCCAGGCCCGCCCTCGCCCCAATGGGGCC
  CCCTGGCCCCCTGAGCCAGCGCCTGCCCCAGCTCCAGAGTTGGACCCCTTTGAGGCCCAGTGGGCGGCAT
  TAGAAGGCAAAGCCACTGTAGAGAAACCCTCCAACCCCTTTTCTGGCGACCTGCAAAAGACATTCGAGAT
  TGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216869 representing NM_004756
  Red=Cloning site Green=Tags(s)
  
  MSRSAAASGGPRRPERHLPPAPCGAPGPPETCRTEPDGAGTMNKLRQSLRRRKPAYVPEASRPHQWQADE
  DAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQT
  IEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTA
  AFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPTVAPGPAQPGHVSPTPATTSPGEKGEAGTP
  VAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKRQLSLRLNELPSTLQRRTDFQVKGTVPEME
  PPGAGDSDSINALCTQISSSFASAGAPAPGPPPATTGTSAWGEPSVPPAAAFQPGHKRTPSEAERWLEEV
  SQVAKAQQQQQQQQQQQQQQQQQQQQAASVAPVPTMPPALQPFPAPVGPFDAAPAQVAVFLPPPHMQPPF
  VPAYPGLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPATLLGKAGAFPPPAIPSAPGSQARPRPNGA
  PWPPEPAPAPAPELDPFEAQWAALEGKATVEKPSNPFSGDLQKTFEIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004756
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004756.1, NM_004756.2, NM_004756.3, NM_004756.4, NP_004747.1
• RefSeq Size: 3568
• RefSeq ORF: 1830
• Locus ID: 9253
• Domains: PID
• Protein Pathways: Notch signaling pathway
• MW: 64.9 kDa
• Gene Summary: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. [UniProtKB/Swiss-Prot Function]