HMX2 (NM_005519) Human Tagged ORF Clone

CAT#: RC216886

HMX2 (Myc-DDK-tagged)-Human H6 family homeobox 2 (HMX2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401694) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216886 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HMX2
• Synonyms: H6L; Nkx5-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216886 representing NM_005519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCAAAGAAGATGCGGGCAAGGGGTGTCCGGCGGCCGGTGGCGTCTCCAGCTTCACCATCCAGT
  CCATCCTGGGCGGGGGCCCCTCGGAGGCACCGCGGGAGCCCGTCGGCTGGCCAGCCAGGAAGCGCAGCCT
  GTCCGTGTCCTCGGAGGAGGAGGAGCCGGACGACGGCTGGAAGGCGCCCGCCTGCTTCTGCCCAGACCAG
  CACGGCCCTAAGGAGCAGGGCCCCAAGCACCATCCCCCCATCCCTTTTCCTTGCCTGGGTACCCCCAAGG
  GCAGCGGAGGCTCGGGCCCGGGCGGCTTGGAGCGCACGCCTTTCCTCTCTCCTTCGCACTCGGACTTTAA
  AGAAGAGAAAGAGAGGCTCCTGCCCGCGGGCTCGCCCTCGCCGGGGTCCGAGCGGCCGCGGGACGGCGGC
  GCTGAGCGGCAGGCCGGCGCGGCCAAGAAGAAGACGCGCACCGTCTTTTCGCGCAGCCAGGTGTACCAGC
  TCGAGTCCACCTTCGACATGAAGCGCTACCTGAGCAGCTCGGAGCGCGCCTGCCTCGCCTCCAGCCTGCA
  GCTCACGGAGACCCAGGTAAAGACTTGGTTCCAGAACCGCCGCAACAAGTGGAAGCGGCAGCTCTCGGCT
  GAGCTGGAGGCGGCCAACATGGCGCACGCGTCGGCGCAGACTCTGGTGAGCATGCCGCTGGTGTTCCGGG
  ACAGTTCGCTGCTGCGCGTGCCGGTGCCGCGCTCGCTCGCCTTTCCCGCGCCGCTCTACTACCCGGGAAG
  CAACCTCTCGGCCTTACCTCTCTACAACCTATACAACAAGCTCGACTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216886 representing NM_005519
  Red=Cloning site Green=Tags(s)
  
  MGSKEDAGKGCPAAGGVSSFTIQSILGGGPSEAPREPVGWPARKRSLSVSSEEEEPDDGWKAPACFCPDQ
  HGPKEQGPKHHPPIPFPCLGTPKGSGGSGPGGLERTPFLSPSHSDFKEEKERLLPAGSPSPGSERPRDGG
  AERQAGAAKKKTRTVFSRSQVYQLESTFDMKRYLSSSERACLASSLQLTETQVKTWFQNRRNKWKRQLSA
  ELEAANMAHASAQTLVSMPLVFRDSSLLRVPVPRSLAFPAPLYYPGSNLSALPLYNLYNKLDY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005519
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005519.1, NP_005510.1
• RefSeq Size: 1628 bp
• RefSeq ORF: 822 bp
• Locus ID: 3167
• Cytogenetics: 10q26.13
• MW: 29.4 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the NKL homeobox family of transcription factors. Members in this family are of ancient origin and play an important role in organ development during embryogenesis. A related mouse protein plays a role in patterning of inner ear structures. In humans, variations in a region containing this gene have been associated with inner ear malformations, vestibular dysfunction, and hearing loss. [provided by RefSeq, Aug 2012]'