PHETA2 (NM_001002034) Human Tagged ORF Clone

CAT#: RC216895

FAM109B (Myc-DDK-tagged)-Human family with sequence similarity 109, member B (FAM109B)

Product Images

Western blot validation of overexpression lysate (Cat# LY424173) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216895 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PHETA2 protein (Cat# TP316895). The protein was produced from HEK293T cells transfected with PHETA2 cDNA clone (Cat# RC216895) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHETA2
• Synonyms: FAM109B; IPIP27B; Ses2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216895 representing NM_001002034
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGAACGAGAGGAGTGTAGCCCACTATGCACTCAGCGACTCCCCAGCGGACCACATGGGCTTCC
  TGCGCACCTGGGGGGGCCCAGGGACCCCACCGACCCCCAGTGGCACTGGCCGAAGATGCTGGTTTGTCCT
  CAAGGGCAACCTGCTATTCTCCTTTGAGAGTCGCGAGGGCCGGGCCCCACTGAGCCTGGTGGTGCTGGAA
  GGCTGCACAGTGGAACTGGCCGAGGCTCCCGTGCCCGAGGAGTTTGCCTTTGCCATCTGCTTTGATGCCC
  CTGGAGTGCGCCCACACCTGCTGGCCGCAGAAGGGCCGGCGGCCCAGGAGGCCTGGGTGAAGGTGCTGTC
  CCGGGCAAGCTTTGGCTACATGCGCCTGGTGGTACGCGAGTTGGAGAGCCAGTTGCAGGACGCACGCCAG
  AGCCTGGCTTTGCAACGCCGCTCATCCTGGAAGTCTGTTGCCAGCCGCTGTAAGCCCCAGGCTCCTAACC
  ACCGAGCTGCGGGCCTGGAGAATGGCCACTGCCTCTCCAAGGACAGCAGCCCTGTGGGCTTGGTTGAAGA
  AGCGGGCAGCAGGTCTGCAGGGTGGGGGTTGGCTGAGTGGGAGCTGCAGGGCCCTGCCAGCCTCCTCCTA
  GGCAAGGGGCAGAGCCCTGTGTCCCCTGAGACCTCCTGCTTCTCTACCCTGCATGACTGGTATGGCCAGG
  AGATCGTGGAGCTGCGGCAGTGTTGGCAGAAGAGGGCCCAGGGGAGCCACTCAAAATGTGAGGAACAGGA
  TAGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216895 representing NM_001002034
  Red=Cloning site Green=Tags(s)
  
  MKLNERSVAHYALSDSPADHMGFLRTWGGPGTPPTPSGTGRRCWFVLKGNLLFSFESREGRAPLSLVVLE
  GCTVELAEAPVPEEFAFAICFDAPGVRPHLLAAEGPAAQEAWVKVLSRASFGYMRLVVRELESQLQDARQ
  SLALQRRSSWKSVASRCKPQAPNHRAAGLENGHCLSKDSSPVGLVEEAGSRSAGWGLAEWELQGPASLLL
  GKGQSPVSPETSCFSTLHDWYGQEIVELRQCWQKRAQGSHSKCEEQDRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002034
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002034.1, NM_001002034.2, NP_001002034.1, NP_001002034.2
• RefSeq Size: 2396
• RefSeq ORF: 780
• Locus ID: 150368
• MW: 28.2 kDa