ALG9 (NM_001077690) Human Tagged ORF Clone

CAT#: RC216941

ALG9 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae) (ALG9), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALG9
• Synonyms: CDG1L; DIBD1; GIKANIS; LOH11CR1J
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216941 representing NM_001077690
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGTCGAGGGGCTCGGCAGCGCCTGAAGGGCAGCGGGGCCAGCAGTGGGGATACGGCCCCGGCTG
  CGGACAAGCTGCGGGAGCTGCTGGGCAGCCGAGAGGCGGGCGGCGCGGAGCACCGGACCGAGTTATCTGG
  GAACAAAGCAGGACAAGTCTGGGCACCTGAAGGATCTACTGCTTTCAAGTGTCTGCTTTCAGCAAGGTTA
  TGTGCTGCTCTCCTGAGCAACATCTCTGACTGTGATGAAACATTCAACTACTGGGAGCCAACACACTACC
  TCATCTATGGGGAAGGGTTTCAGACTTGGGAATATTCCCCAGCATATGCCATTCGCTCCTATGCTTACCT
  GTTGCTTCATGCCTGGCCAGCTGCATTTCATGCAAGAATTCTACAAACTAATAAGATTCTTGTGTTTTAC
  TTTTTGCGATGTCTTCTGGCTTTTGTGAGCTGTATTTGTGAACTTTACTTTTACAAGGCTGTGTGCAAGA
  AGTTTGGGTTGCACGTGAGTCGAATGATGCTAGCCTTCTTGGTTCTCAGCACTGGCATGTTTTGCTCATC
  ATCAGCATTCCTTCCTAGTAGCTTCTGTATGTACACTACGTTGATAGCCATGACTGGATGGTATATGGAC
  AAGACTTCCATTGCTGTGCTGGGAGTAGCAGCTGGGGCTATCTTAGGCTGGCCATTCAGTGCAGCTCTTG
  GTTTACCCATTGCCTTTGATTTGCTGGTCATGAAACACAGGTGGAAGAGTTTCTTTCATTGGTCGCTGAT
  GGCCCTCATACTATTTCTGGTGCCTGTGGTGGTCATTGACAGCTACTATTATGGGAAGTTGGTGATTGCA
  CCACTCAACATTGTTTTGTATAATGTCTTTACTCCTCATGGACCTGATCTTTATGGTACAGAACCCTGGT
  ATTTCTATTTAATTAATGGATTTCTGAATTTCAATGTAGCCTTTGCTTTGGCTCTCCTAGTCCTACCACT
  GACTTCTCTTATGGAATACCTGCTGCAGAGATTTCATGTTCAGAATTTAGGCCACCCGTATTGGCTTACC
  TTGGCTCCAATGTATATTTGGTTTATAATTTTCTTCATCCAGCCTCACAAAGAGGAGAGATTTCTTTTCC
  CTGTGTATCCACTTATATGTCTCTGTGGCGCTGTGGCTCTCTCTGCACTTCAGAAATGTTACCACTTTGT
  GTTTCAACGATATCGCCTGGAGCACTATACTGTGACATCGAATTGGCTGGCATTAGGAACTGTCTTCCTG
  TTTGGGCTCTTGTCATTTTCTCGCTCTGTGGCACTGTTCAGAGGATATCACGGGCCCCTTGATTTGTATC
  CAGAATTTTACCGAATTGCTACAGACCCAACCATCCACACTGTCCCAGAAGGCAGACCTGTGAATGTCTG
  TGTGGGAAAAGAGTGGTATCGATTTCCCAGCAGCTTCCTTCTTCCTGACAATTGGCAGCTTCAGTTCATT
  CCATCAGAGTTCAGAGGTCAGTTACCAAAACCTTTTGCAGAAGGACCTCTGGCCACCCGGATTGTTCCTA
  CTGACATGAATGACCAGAATCTAGAAGAGCCATCCAGATATATTGATATCAGTAAATGCCATTATTTAGT
  GGATTTGGACACCATGAGAGAAACACCCCGGGAGCCAAAATATTCATCCAATAAAGAAGAATGGATCAGC
  TTGGCCTATAGACCATTCCTTGATGCTTCTAGATCTTCAAAGCTGCTGCGGGCATTCTATGTCCCCTTCC
  TGTCAGATCAGTATACAGTGTACGTAAACTACACCATCCTCAAACCCCGGAAAGCAAAGCAAATCAGGAA
  GAAAAGTGGAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216941 representing NM_001077690
  Red=Cloning site Green=Tags(s)
  
  MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTELSGNKAGQVWAPEGSTAFKCLLSARL
  CAALLSNISDCDETFNYWEPTHYLIYGEGFQTWEYSPAYAIRSYAYLLLHAWPAAFHARILQTNKILVFY
  FLRCLLAFVSCICELYFYKAVCKKFGLHVSRMMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMD
  KTSIAVLGVAAGAILGWPFSAALGLPIAFDLLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIA
  PLNIVLYNVFTPHGPDLYGTEPWYFYLINGFLNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPYWLT
  LAPMYIWFIIFFIQPHKEERFLFPVYPLICLCGAVALSALQKCYHFVFQRYRLEHYTVTSNWLALGTVFL
  FGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPEGRPVNVCVGKEWYRFPSSFLLPDNWQLQFI
  PSEFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLDTMRETPREPKYSSNKEEWIS
  LAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVNYTILKPRKAKQIRKKSGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077690
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077690.1, NP_001071158.1
• RefSeq Size: 6137
• RefSeq ORF: 1836
• Locus ID: 79796
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 69.7 kDa
• Gene Summary: This gene encodes an alpha-1,2-mannosyltransferase enzyme that functions in lipid-linked oligosaccharide assembly. Mutations in this gene result in congenital disorder of glycosylation type Il. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]