ABHD12 (NM_001042472) Human Tagged ORF Clone

CAT#: RC216963

ABHD12 (Myc-DDK-tagged)-Human abhydrolase domain containing 12 (ABHD12), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420926) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216963 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ABHD12 protein (Cat# TP316963). The protein was produced from HEK293T cells transfected with ABHD12 cDNA clone (Cat# RC216963) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD12
• Synonyms: ABHD12A; BEM46L2; C20orf22; dJ965G21.2; hABHD12; PHARC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216963 representing NM_001042472
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGCGGACCGAGCCCGTCGCCTTGGAGCATGAGCGCTGCGCCGCCGCGGGCTCGTCCTCCTCCG
  GCTCGGCCGCCGCGGCGCTGGACGCCGACTGCCGCCTGAAGCAGAACCTACGCCTGACGGGCCCGGCGGC
  GGCTGAGCCGCGCTGCGCAGCCGACGCGGGAATGAAGCGGGCGCTGGGCAGGCGAAAGGGCGTGTGGTTG
  CGCCTGAGGAAGATACTTTTCTGTGTTTTGGGGTTGTACATTGCCATTCCATTTCTCATCAAACTATGTC
  CTGGAATACAGGCCAAACTGATTTTCTTGAATTTCGTAAGAGTTCCCTATTTCATTGATTTGAAAAAACC
  ACAGGATCAAGGTTTGAATCACACGTGTAACTACTACCTGCAGCCAGAGGAAGACGTGACCATTGGAGTC
  TGGCACACCGTCCCTGCAGTCTGGTGGAAGAACGCCCAAGGCAAAGACCAGATGTGGTATGAGGATGCCT
  TGGCTTCCAGCCACCCTATCATTCTGTACCTGCATGGGAACGCAGGTACCAGAGGAGGCGACCACCGCGT
  GGAGCTTTACAAGGTGCTGAGTTCCCTTGGTTACCATGTGGTCACCTTTGACTACAGAGGTTGGGGTGAC
  TCAGTGGGAACGCCATCTGAGCGGGGCATGACCTATGACGCACTCCACGTTTTTGACTGGATCAAAGCAA
  GAAGTGGTGACAACCCCGTGTACATCTGGGGCCACTCTCTGGGCACTGGCGTGGCGACAAATCTGGTGCG
  GCGCCTCTGTGAGCGAGAGACGCCTCCAGATGCCCTTATATTGGAATCTCCATTCACTAATATCCGCGAA
  GAAGCTAAGAGCCATCCATTTTCAGTGATATATCGATACTTCCCTGGGTTTGACTGGTTCTTCCTTGATC
  CTATTACAAGTAGTGGAATTAAATTTGCAAATGATGAAAACGTGAAGCACATCTCCTGTCCCCTGCTCAT
  CCTGCACGCTGAGGACGACCCGGTGGTGCCCTTCCAGCTTGGCAGAAAGCTCTATAGCATCGCCGCACCA
  GCTCGAAGCTTCCGAGATTTCAAAGTTCAGTTTGTGCCCTTTCATTCAGACCTTGGCTACAGGCACAAAT
  ACATTTACAAGAGCCCTGAGCTGCCACGGATACTGAGGGAATTCCTGGGGAAGTCGGAGCCTGAGCACCA
  GCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216963 representing NM_001042472
  Red=Cloning site Green=Tags(s)
  
  MRKRTEPVALEHERCAAAGSSSSGSAAAALDADCRLKQNLRLTGPAAAEPRCAADAGMKRALGRRKGVWL
  RLRKILFCVLGLYIAIPFLIKLCPGIQAKLIFLNFVRVPYFIDLKKPQDQGLNHTCNYYLQPEEDVTIGV
  WHTVPAVWWKNAQGKDQMWYEDALASSHPIILYLHGNAGTRGGDHRVELYKVLSSLGYHVVTFDYRGWGD
  SVGTPSERGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGVATNLVRRLCERETPPDALILESPFTNIRE
  EAKSHPFSVIYRYFPGFDWFFLDPITSSGIKFANDENVKHISCPLLILHAEDDPVVPFQLGRKLYSIAAP
  ARSFRDFKVQFVPFHSDLGYRHKYIYKSPELPRILREFLGKSEPEHQH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042472
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042472.1, NM_001042472.2, NP_001035937.1
• RefSeq Size: 1983
• RefSeq ORF: 1197
• Locus ID: 26090
• Protein Families: Protease, Transmembrane
• MW: 44.9 kDa
• Gene Summary: This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG), the main endocannabinoid lipid transmitter that acts on cannabinoid receptors, CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes, including neurotransmission, mood, appetite, pain appreciation, addiction behavior, and inflammation. Mutations in this gene are associated with the neurodegenerative disease, PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract), resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2011]