CYP26C1 (NM_183374) Human Tagged ORF Clone

CAT#: RC216976

CYP26C1 (Myc-DDK-tagged)-Human cytochrome P450, family 26, subfamily C, polypeptide 1 (CYP26C1)

Product Images

Western blot validation of overexpression lysate (Cat# LY405254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216976 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CYP26C1 protein (Cat# TP316976). The protein was produced from HEK293T cells transfected with CYP26C1 cDNA clone (Cat# RC216976) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP26C1
• Synonyms: FFDD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216976 representing NM_183374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCTTGGGGGCTGAGCTGCCTGTCAGTGCTGGGGGCGGCGGGCACTGCTCTCCTGTGCGCGGGCC
  TGCTGCTCAGCCTGGCCCAGCACCTCTGGACCCTCCGCTGGATGCTGAGCCGGGACCGGGCCTCCACCCT
  GCCTCTGCCCAAGGGCTCCATGGGGTGGCCCTTCTTCGGCGAAACGCTGCACTGGTTAGTTCAGGGCTCG
  CGCTTCCACAGTTCTCGCCGAGAGCGCTATGGGACAGTGTTCAAGACGCACCTGCTGGGCAGGCCAGTGA
  TCCGCGTGAGCGGCGCGGAGAACGTGCGCACCATCCTGCTGGGCGAGCACCGCCTGGTGCGCAGCCAGTG
  GCCGCAGAGTGCGCACATCCTGCTGGGCTCGCACACACTGCTAGGTGCGGTCGGCGAGCCGCACCGGCGG
  CGGCGCAAGGTCCTGGCGCGCGTGTTCAGCCGCGCCGCGCTGGAGCGCTACGTGCCGCGCCTGCAGGGGG
  CGCTGCGGCATGAGGTGCGCTCCTGGTGCGCGGCGGGCGGGCCGGTCTCAGTCTACGACGCCTCCAAAGC
  GCTCACCTTCCGCATGGCCGCGCGCATCCTGCTGGGGTTGCGGCTGGACGAGGCGCAGTGCGCCACGCTG
  GCCCGGACCTTCGAGCAGCTCGTGGAGAACCTCTTCTCACTGCCTCTGGACGTTCCCTTCAGTGGCCTAC
  GCAAGGGCATCCGGGCAAGGGACCAGCTGCATCGGCACCTGGAGGGGGCCATTTCTGAGAAGCTTCACGA
  GGACAAGGCTGCAGAGCCGGGTGATGCCCTCGACCTAATCATTCACAGTGCAAGGGAGCTGGGCCATGAG
  CCCTCCATGCAGGAGCTGAAGGAGTCGGCTGTGGAGCTCCTCTTCGCCGCCTTCTTCACCACGGCCAGTG
  CCAGCACCTCGCTCGTCCTGCTGCTACTGCAGCATCCGGCGGCCATCGCCAAGATTCGGGAGGAGCTGGT
  GGCGCAGGGGCTGGGGCGCGCGTGCGGCTGCGCGCCCGGGGCCGCTGGGGGCAGCGAGGGGCCCCCGCCC
  GACTGCGGCTGCGAGCCCGACCTCAGCCTCGCGGCGCTGGGCCGTCTGCGCTACGTCGACTGCGTGGTCA
  AGGAGGTGCTGCGCCTCCTGCCGCCAGTGTCCGGGGGCTACCGCACCGCCCTGCGCACCTTCGAGCTCGA
  CGGCTACCAGATCCCCAAGGGCTGGAGCGTGATGTATAGCATCCGGGACACGCACGAGACGGCTGCGGTG
  TACCGCAGCCCTCCCGAAGGCTTCGATCCAGAGCGCTTCGGCGCAGCGCGCGAAGATTCCCGGGGCGCCT
  CCAGCCGCTTCCATTACATCCCGTTCGGCGGCGGTGCGCGCAGCTGCCTCGGCCAGGAGCTGGCGCAAGC
  CGTGCTCCAGCTGCTAGCTGTGGAGCTAGTGCGCACCGCGCGCTGGGAACTGGCCACACCCGCCTTCCCC
  GCCATGCAGACGGTGCCCATCGTGCACCCAGTGGACGGGCTGCGGCTCTTTTTCCACCCCCTCACGCCTT
  CGGTTGCGGGGAATGGGCTATGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216976 representing NM_183374
  Red=Cloning site Green=Tags(s)
  
  MFPWGLSCLSVLGAAGTALLCAGLLLSLAQHLWTLRWMLSRDRASTLPLPKGSMGWPFFGETLHWLVQGS
  RFHSSRRERYGTVFKTHLLGRPVIRVSGAENVRTILLGEHRLVRSQWPQSAHILLGSHTLLGAVGEPHRR
  RRKVLARVFSRAALERYVPRLQGALRHEVRSWCAAGGPVSVYDASKALTFRMAARILLGLRLDEAQCATL
  ARTFEQLVENLFSLPLDVPFSGLRKGIRARDQLHRHLEGAISEKLHEDKAAEPGDALDLIIHSARELGHE
  PSMQELKESAVELLFAAFFTTASASTSLVLLLLQHPAAIAKIREELVAQGLGRACGCAPGAAGGSEGPPP
  DCGCEPDLSLAALGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELDGYQIPKGWSVMYSIRDTHETAAV
  YRSPPEGFDPERFGAAREDSRGASSRFHYIPFGGGARSCLGQELAQAVLQLLAVELVRTARWELATPAFP
  AMQTVPIVHPVDGLRLFFHPLTPSVAGNGLCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183374
• ORF Size: 1566 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183374.1, NM_183374.2, NP_899230.2
• RefSeq Size: 1569
• RefSeq ORF: 1569
• Locus ID: 340665
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Retinol metabolism
• MW: 56.9 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is involved in the catabolism of all-trans- and 9-cis-retinoic acid, and thus contributes to the regulation of retinoic acid levels in cells and tissues. This gene is adjacent to a related gene on chromosome 10q23.33. [provided by RefSeq, Jul 2008]