Apolipoprotein L 1 (APOL1) (NM_145343) Human Tagged ORF Clone

CAT#: RC217000

APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY407931) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217000 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APOL1
• Synonyms: APO-L; APOL; APOL-I; FSGS4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217000 representing NM_145343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATTCAAAAGCCACACTGTGGAATTGAGGAGGCCCTGCAGCGACATGGAGGGAGCTGCTTTGCTGA
  GAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTCCTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGG
  AGCGAGGGTGCAACAAAACGTTCCAAGTGGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGAC
  TGGGCTGCTGGCACCATGGACCCAGAGAGCAGTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAA
  AAGTGAGCACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCTGC
  TGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGACAAATGATCATG
  AAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTTCTGAAAGAGTTTCCTCGGTTGA
  AAAGTGAGCTTGAGGATAACATAAGAAGGCTCCGTGCCCTTGCAGATGGGGTTCAGAAGGTCCACAAAGG
  CACCACCATCGCCAATGTGGTGTCTGGCTCTCTCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATG
  GGTCTGGCACCCTTCACAGAGGGAGGCAGCCTTGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAG
  CCGCTTTGACCGGGATTACCAGCAGTACCATGGACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCCCA
  CGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAGGGAGTTTTTGGGTGAGAACATATCCAAC
  TTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGGAAGGACATCCGTGCCCTCAGAC
  GAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCAGCCTCACGCCCCCGGGTCACTGAGCCAATCTC
  AGCTGAAAGCGGTGAACAGGTGGAGAGGGTTAATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAG
  CTCACGGATGTGGCCCCTGTAAGCTTCTTTCTTGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGC
  ACTTACATGAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAGAA
  GCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217000 representing NM_145343
  Red=Cloning site Green=Tags(s)
  
  MRFKSHTVELRRPCSDMEGAALLRVSVLCIWMSALFLGVGVRAEEAGARVQQNVPSGTDTGDPQSKPLGD
  WAAGTMDPESSIFIEDAIKYFKEKVSTQNLLLLLTDNEAWNGFVAAAELPRNEADELRKALDNLARQMIM
  KDKNWHDKGQQYRNWFLKEFPRLKSELEDNIRRLRALADGVQKVHKGTTIANVVSGSLSISSGILTLVGM
  GLAPFTEGGSLVLLEPGMELGITAALTGITSSTMDYGKKWWTQAQAHDLVIKSLDKLKEVREFLGENISN
  FLSLAGNTYQLTRGIGKDIRALRRARANLQSVPHASASRPRVTEPISAESGEQVERVNEPSILEMSRGVK
  LTDVAPVSFFLVLDVVYLVYESKHLHEGAKSETAEELKKVAQELEEKLNILNNNYKILQADQEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145343
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145343.1, NM_145343.2, NP_663318.1
• RefSeq Size: 3010
• RefSeq ORF: 1245
• Locus ID: 8542
• Protein Families: Secreted Protein, Transmembrane
• MW: 41.1 kDa
• Gene Summary: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]