UCKL1 (NM_017859) Human Tagged ORF Clone

CAT#: RC217118

UCKL1 (Myc-DDK-tagged)-Human uridine-cytidine kinase 1-like 1 (UCKL1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413319) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217118 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UCKL1
• Synonyms: UCK1L; URKL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217118 representing NM_017859
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCCCCCGGCCCGCGCGGACGCTGATCCTTCGCCCACGTCGCCACCTACGGCCCGAGACACAC
  CAGGCCGGCAGGCTGAGAAAAGCGAGACCGCGTGCGAGGACCGCAATGCAGAGTCCCTGGACAGGCTCCT
  GCCACCTGTGGGCACTGGGCGCTCTCCCCGGAAGCGGACCACCAGCCAGTGCAAGTCAGAGCCTCCCCTG
  CTGCGTACAAGCAAGCGTACCATCTACACCGCCGGGCGGCCGCCCTGGTACAATGAACACGGCACGCAAT
  CCAAAGAGGCCTTCGCCATCGGCTTGGGAGGCGGCAGTGCCTCTGGGAAGACCACTGTGGCCAGAATGAT
  CATCGAGGCCCTGGATGTGCCCTGGGTGGTCTTGCTGTCCATGGACTCCTTCTACAAGGTGCTGACTGAG
  CAGCAGCAGGAACAGGCCGCACACAACAACTTCAACTTCGACCACCCAGATGCCTTTGACTTCGACCTCA
  TCATTTCCACCCTCAAGAAGCTGAAGCAGGGGAAGAGTGTCAAGGTGCCCATTTATGACTTCACCACGCA
  CAGCCGGAAGAAGGACTGGAAAACACTGTATGGTGCAAACGTCATCATCTTTGAGGGCATCATGGCCTTT
  GCTGACAAGACACTGTTGGAGCTCCTGGACATGAAGATCTTTGTGGACACAGACTCCGACATCCGCCTGG
  TACGGCGGCTGCGCCGGGACATCAGTGAGCGCGGCCGGGACATCGAGGGTGTCATCAAGCAGTACAACAA
  GTTTGTCAAGCCCTCCTTCGACCAGTACATCCAGCCCACCATGCGCCTGGCAGACATCGTGGTCCCCAGA
  GGGAGCGGCAACACGGTGGCCATCGACCTGATTGTGCAGCACGTGCACAGCCAGCTGGAGGAGCGTGAAC
  TCAGCGTCAGGGCTGCGCTGGCCTCGGCACACCAGTGCCACCCGCTGCCCCGGACGCTGAGCGTCCTGAA
  GAGCACGCCGCAGGTACGGGGCATGCACACCATCATCAGGGACAAGGAGACCAGTCGCGACGAGTTCATC
  TTCTACTCCAAGAGACTGATGCGGCTGCTCATCGAGCACGCGCTCTCCTTCCTGCCCTTTCAGGACTGCG
  TCGTACAGACCCCGCAGGGGCAGGACTATGCGGGCAAGTGCTATGCGGGGAAGCAGATCACCGGTGTGTC
  CATTCTGCGCGCCGGTGAAACCATGGAGCCCGCGCTGCGCGCTGTGTGCAAAGACGTGCGCATCGGCACC
  ATCCTCATCCAGACCAACCAGCTTACCGGGGAGCCCGAGCTCCACTACCTGAGGCTGCCCAAGGACATCA
  GCGATGACCACGTGATCCTCATGGACTGCACCGTGTCCACGGGCGCGGCGGCCATGATGGCAGTGCGCGT
  GCTCCTGGACCACGACGTGCCTGAGGACAAGATCTTTTTGCTGTCGCTGCTCATGGCAGAGATGGGCGTG
  CACTCAGTGGCCTATGCATTTCCGCGAGTGAGAATCATCACCACGGCGGTGGACAAGCGGGTCAATGACC
  TTTTCCGCATCATCCCAGGCATTGGGAACTTTGGCGACCGCTACTTTGGGACAGACGCGGTCCCCGATGG
  CAGTGACGAGGAGGAAGTGGCCTACACGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217118 representing NM_017859
  Red=Cloning site Green=Tags(s)
  
  MAAPPARADADPSPTSPPTARDTPGRQAEKSETACEDRNAESLDRLLPPVGTGRSPRKRTTSQCKSEPPL
  LRTSKRTIYTAGRPPWYNEHGTQSKEAFAIGLGGGSASGKTTVARMIIEALDVPWVVLLSMDSFYKVLTE
  QQQEQAAHNNFNFDHPDAFDFDLIISTLKKLKQGKSVKVPIYDFTTHSRKKDWKTLYGANVIIFEGIMAF
  ADKTLLELLDMKIFVDTDSDIRLVRRLRRDISERGRDIEGVIKQYNKFVKPSFDQYIQPTMRLADIVVPR
  GSGNTVAIDLIVQHVHSQLEERELSVRAALASAHQCHPLPRTLSVLKSTPQVRGMHTIIRDKETSRDEFI
  FYSKRLMRLLIEHALSFLPFQDCVVQTPQGQDYAGKCYAGKQITGVSILRAGETMEPALRAVCKDVRIGT
  ILIQTNQLTGEPELHYLRLPKDISDDHVILMDCTVSTGAAAMMAVRVLLDHDVPEDKIFLLSLLMAEMGV
  HSVAYAFPRVRIITTAVDKRVNDLFRIIPGIGNFGDRYFGTDAVPDGSDEEEVAYTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017859
• ORF Size: 1103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017859.1, NM_017859.2, NM_017859.3, NP_060329.2
• RefSeq Size: 1833 bp
• RefSeq ORF: 1647 bp
• Locus ID: 54963
• Domains: PRK
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• MW: 61 kDa
• Gene Summary: The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]