AFMID (NM_001010982) Human Tagged ORF Clone

CAT#: RC217126

AFMID (Myc-DDK-tagged)-Human arylformamidase (AFMID), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY423254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217126 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AFMID protein (Cat# TP317126). The protein was produced from HEK293T cells transfected with AFMID cDNA clone (Cat# RC217126) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFMID
• Synonyms: FKF; KF; KFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217126 representing NM_001010982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATGTGTCTGGTGTGGGTTTCCCAAGCAAGGTTCCTTGGAAGAAGATGTCTGCAGAGGAGCTGG
  AGAATCAGTACTGTCCCAGCCGATGGGTTGTCCGACTGGGAGCAGAGGAAGCCTTGAGGACCTACTCACA
  GATAGGAATTGAAGCCACCACAAGGGCCCGGGCCACCAGGAAGAGCCTGCTGCATGTCCCCTATGGAGAC
  GGCGAAGGGGAGAAAGTGGACATTTACTTCCCCGACGAGTCGTCTGAAGCCTTGCCTTTCTTCCTGTTCT
  TTCACGGAGGATACTGGCAGAGCGGAAGTAAGGATGAGTCTGCCTTCATGGTCCACCCGCTGACGGCACA
  GGGAGTGGCCGTGGTAATAGTGGCTTACGGCATCGCCCCCAAAGGCACCCTGGACCACATGGTAGACCAG
  GTGACCCGCAGCGTTGCGTTTGTCCAGAAGCGGTATCCAAGCAACAAGGGAATTTACCTGTGTGGACACT
  CAGCCGGGGCCCACCTGGCTGCCATGATGCTCCTGGCCGACTGGACCAAGCATGGGGTCACGCCCAACCT
  CAGAGGCTTTTTCCTGGTGAGTGGGGTCTTTGACCTGGAGCCCATCGTGTATACTTCACAGAACGTTGCT
  CTCCAGCTGACCCTGGAGGACGCTCAGAGGAATAGCCCCCAGCTGAAGGTGGCCCAGGCACAGCCGGTGG
  ACCCCACCTGCCGTGTGCTGGTGGTCGTGGGCCAGTTCGACTCCCCCGAATTCCACCGACAGTCCTGGGA
  GTTTTACCAGACCCTGTGTCAAGGAGAGTGGAAAGCCTCATTTGAAGAGCTCCACGATGTGGACCACTTT
  GAAATTGTTGAGAATCTGACCCAGAAGGACAACGTGCTCACCCAGATTATCTTGAAAACAATCTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217126 representing NM_001010982
  Red=Cloning site Green=Tags(s)
  
  MMDVSGVGFPSKVPWKKMSAEELENQYCPSRWVVRLGAEEALRTYSQIGIEATTRARATRKSLLHVPYGD
  GEGEKVDIYFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQ
  VTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWTKHGVTPNLRGFFLVSGVFDLEPIVYTSQNVA
  LQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQTLCQGEWKASFEELHDVDHF
  EIVENLTQKDNVLTQIILKTIFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010982
• ORF Size: 1268 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010982.3, NM_001010982.4
• RefSeq Size: 1820
• RefSeq ORF: 912
• Locus ID: 125061
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Tryptophan metabolism
• MW: 33.8 kDa