PACE4 (PCSK6) (NM_002570) Human Tagged ORF Clone

CAT#: RC217128

PCSK6 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCSK6
• Synonyms: PACE4; SPC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217128 representing NM_002570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCGCGCGCGCCGCCTGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGCCGCCACCGACACCG
  CCGCGGGCGCGGGGGGCGCGGGGGGCGCGGGGGGCGCCGGCGGGCCCGGGTTCCGGCCGCTCGCGCCGCG
  TCCCTGGCGCTGGCTGCTGCTGCTGGCGCTGCCTGCCGCCTGCTCCGCGCCCCCGCCGCGCCCCGTCTAC
  ACCAACCACTGGGCGGTGCAAGTGCTGGGCGGCCCGGCCGAGGCGGACCGCGTGGCGGCGGCGCACGGGT
  ACCTCAACTTGGGCCAGATTGGAAACCTGGAAGATTACTACCATTTTTATCACAGCAAAACCTTTAAAAG
  ATCAACCTTGAGTAGCAGAGGCCCTCACACCTTCCTCAGAATGGACCCCCAGGTGAAATGGCTCCAGCAA
  CAGGAAGTGAAACGAAGGGTGAAGAGACAGGTGCGAAGTGACCCGCAGGCCCTTTACTTCAACGACCCCA
  TTTGGTCCAACATGTGGTACCTGCATTGTGGCGACAAGAACAGTCGCTGCCGGTCGGAAATGAATGTCCA
  GGCAGCGTGGAAGAGGGGCTACACAGGAAAAAACGTGGTGGTCACCATCCTTGATGATGGCATAGAGAGA
  AATCACCCTGACCTGGCCCCAAATTATGATTCCTACGCCAGCTACGACGTGAACGGCAATGATTATGACC
  CATCTCCACGATATGATGCCAGCAATGAAAATAAACACGGCACTCGTTGTGCGGGAGAAGTTGCTGCTTC
  AGCAAACAATTCCTACTGCATCGTGGGCATAGCGTACAATGCCAAAATAGGAGGCATCCGCATGCTGGAC
  GGCGATGTCACAGATGTGGTCGAGGCAAAGTCGCTGGGCATCAGACCCAACTACATCGACATTTACAGTG
  CCAGCTGGGGGCCGGACGACGACGGCAAGACGGTGGACGGGCCCGGCCGACTGGCTAAGCAGGCTTTCGA
  GTATGGCATTAAAAAGGGCCGGCAGGGCCTGGGCTCCATTTTCGTCTGGGCATCTGGGAATGGCGGGAGA
  GAGGGGGACTACTGCTCGTGCGATGGCTACACCAACAGCATCTACACCATCTCCGTCAGCAGCGCCACCG
  AGAATGGCTACAAGCCCTGGTACCTGGAAGAGTGTGCCTCCACCCTGGCCACCACCTACAGCAGTGGGGC
  CTTTTATGAGCGAAAAATCGTCACCACGGATCTGCGTCAGCGCTGTACCGATGGCCACACTGGGACCTCA
  GTCTCTGCCCCCATGGTGGCGGGCATCATCGCCTTGGCTCTAGAAGCAAACAGCCAGTTAACCTGGAGGG
  ACGTCCAGCACCTGCTAGTGAAGACATCCCGGCCGGCCCACCTGAAAGCGAGCGACTGGAAAGTGAACGG
  CGCGGGTCATAAAGTTAGCCATTTCTATGGATTTGGTTTGGTGGACGCAGAAGCTCTCGTTGTGGAGGCA
  AAGAAGTGGACAGCAGTGCCATCGCAGCACATGTGTGTGGCCGCCTCGGACAAGAGACCCAGGAGCATCC
  CCTTAGTGCAGGTGCTGCGGACTACGGCCCTGACCAGCGCCTGCGCGGAGCACTCGGACCAGCGGGTGGT
  CTACTTGGAGCACGTGGTGGTTCGCACCTCCATCTCACACCCACGCCGAGGAGACCTCCAGATCTACCTG
  GTTTCTCCCTCGGGAACCAAGTCTCAACTTCTGGCAAAGAGGTTGCTGGATCTTTCCAATGAAGGGTTTA
  CAAACTGGGAATTCATGACTGTCCACTGCTGGGGAGAAAAGGCTGAAGGGCAGTGGACCTTGGAAATCCA
  AGATCTGCCATCCCAGGTCCGCAACCCGGAGAAGCAAGGGAAGTTGAAAGAATGGAGCCTCATACTGTAT
  GGCACAGCAGAGCACCCGTACCACACCTTCAGTGCCCATCAGTCCCGCTCGCGGATGCTGGAGCTCTCAG
  CCCCAGAGCTGGAGCCACCCAAGGCTGCCCTGTCACCCTCCCAGGTGGAAGTTCCTGAAGATGAGGAAGA
  TTACACAGCTCAATCCACCCCAGGCTCTGCTAATATTTTACAGACCAGTGTGTGCCATCCGGAGTGTGGT
  GACAAAGGCTGTGATGGCCCCAATGCAGACCAGTGCTTGAACTGCGTCCACTTCAGCCTGGGGAGTGTCA
  AGACCAGCAGGAAGTGCGTGAGTGTGTGCCCCTTGGGCTACTTTGGGGACACAGCAGCAAGACGCTGTCG
  CCGGTGCCACAAGGGGTGTGAGACCTGCTCCAGCAGAGCTGCGACGCAGTGCCTGTCTTGCCGCCGCGGG
  TTCTATCACCACCAGGAGATGAACACCTGTGTGACCCTCTGTCCTGCAGGATTTTATGCTGATGAAAGTC
  AGAAAAATTGCCTTAAATGCCACCCAAGCTGTAAAAAGTGCGTGGATGAACCTGAGAAATGTACTGTCTG
  TAAAGAAGGATTCAGCCTTGCACGGGGCAGCTGCATTCCTGACTGTGAGCCAGGCACCTACTTTGACTCA
  GAGCTGATCAGATGTGGGGAATGCCATCACACCTGCGGAACCTGCGTGGGGCCAGGCAGAGAAGAGTGCA
  TTCACTGTGCGAAAAACTTCCACTTCCACGACTGGAAGTGTGTGCCAGCCTGTGGTGAGGGCTTCTACCC
  AGAAGAGATGCCGGGCTTGCCCCACAAAGTGTGTCGAAGGTGTGACGAGAACTGCTTGAGCTGTGCAGGC
  TCCAGCAGGAACTGTAGCAGGTGTAAGACGGGCTTCACACAGCTGGGGACCTCCTGCATCACCAACCACA
  CGTGCAGCAACGCTGACGAGACATTCTGCGAGATGGTGAAGTCCAACCGGCTGTGCGAACGGAAGCTCTT
  CATTCAGTTCTGCTGCCGCACGTGCCTCCTGGCCGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217128 representing NM_002570
  Red=Cloning site Green=Tags(s)
  
  MPPRAPPAPGPRPPPRAAAATDTAAGAGGAGGAGGAGGPGFRPLAPRPWRWLLLLALPAACSAPPPRPVY
  TNHWAVQVLGGPAEADRVAAAHGYLNLGQIGNLEDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQ
  QEVKRRVKRQVRSDPQALYFNDPIWSNMWYLHCGDKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIER
  NHPDLAPNYDSYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLD
  GDVTDVVEAKSLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGR
  EGDYCSCDGYTNSIYTISVSSATENGYKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTS
  VSAPMVAGIIALALEANSQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHFYGFGLVDAEALVVEA
  KKWTAVPSQHMCVAASDKRPRSIPLVQVLRTTALTSACAEHSDQRVVYLEHVVVRTSISHPRRGDLQIYL
  VSPSGTKSQLLAKRLLDLSNEGFTNWEFMTVHCWGEKAEGQWTLEIQDLPSQVRNPEKQGKLKEWSLILY
  GTAEHPYHTFSAHQSRSRMLELSAPELEPPKAALSPSQVEVPEDEEDYTAQSTPGSANILQTSVCHPECG
  DKGCDGPNADQCLNCVHFSLGSVKTSRKCVSVCPLGYFGDTAARRCRRCHKGCETCSSRAATQCLSCRRG
  FYHHQEMNTCVTLCPAGFYADESQKNCLKCHPSCKKCVDEPEKCTVCKEGFSLARGSCIPDCEPGTYFDS
  ELIRCGECHHTCGTCVGPGREECIHCAKNFHFHDWKCVPACGEGFYPEEMPGLPHKVCRRCDENCLSCAG
  SSRNCSRCKTGFTQLGTSCITNHTCSNADETFCEMVKSNRLCERKLFIQFCCRTCLLAG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002570
• ORF Size: 2907 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002570.1, NM_002570.2, NM_002570.3, NM_002570.4, NP_002561.1
• RefSeq Size: 4559
• RefSeq ORF: 2910
• Locus ID: 5046
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 106.42 kDa
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]