FBXO21 (NM_015002) Human Tagged ORF Clone

CAT#: RC217210

FBXO21 (Myc-DDK-tagged)-Human F-box protein 21 (FBXO21), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXO21
• Synonyms: FBX21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217210 representing NM_015002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGCAGTCGACAGCGCGATGGAGGTGGTGCCGGCGCTGGCGGAGGAGGCCGCGCCGGAGG
  TAGCGGGCCTCAGCTGCCTCGTCAACCTGCCGGGTGAGGTGCTGGAGTACATCCTGTGCTGCGGCTCGCT
  GACGGCCGCCGACATCGGCCGTGTCTCCAGCACCTGCCGGCGGCTGCGCGAGCTGTGCCAGAGCAGCGGG
  AAGGTGTGGAAGGAGCAGTTCCGGGTGAGGTGGCCTTCCCTTATGAAACACTACAGCCCCACCGACTACG
  TCAATTGGTTGGAAGAGTATAAAGTTCGGCAAAAAGCTGGGTTAGAAGCGCGGAAGATTGTAGCCTCGTT
  CTCAAAGAGGTTCTTTTCAGAGCACGTTCCTTGTAATGGCTTCAGTGACATTGAGAACCTTGAAGGACCA
  GAGATTTTTTTTGAGGATGAACTGGTGTGTATCCTAAATATGGAAGGAAGAAAAGCTTTGACCTGGAAAT
  ACTACGCAAAAAAAATTCTTTACTACCTGCGGCAACAGAAGATCTTAAATAATCTTAAGGCCTTTCTTCA
  GCAGCCAGATGACTATGAGTCGTATCTTGAAGGTGCTGTATATATTGACCAGTACTGCAATCCTCTCTCC
  GACATCAGCCTCAAAGACATCCAGGCCCAAATTGACAGCATCGTGGAGCTTGTTTGCAAAACCCTTCGGG
  GCATAAACAGTCGCCACCCCAGCTTGGCCTTCAAGGCAGGTGAATCATCCATGATAATGGAAATAGAACT
  CCAGAGCCAGGTGCTGGATGCCATGAACTATGTCCTTTACGACCAACTGAAGTTCAAGGGGAATCGAATG
  GATTACTATAATGCCCTCAACTTATATATGCATCAGGTTTTGATTCGCAGAACAGGAATCCCAATCAGCA
  TGTCTCTGCTCTATTTGACAATTGCTCGGCAGTTGGGAGTCCCACTGGAGCCTGTCAACTTCCCAAGTCA
  CTTCTTATTAAGGTGGTGCCAAGGCGCAGAAGGGGCGACCCTGGACATCTTTGACTACATCTACATAGAT
  GCTTTTGGGAAAGGCAAGCAGCTGACAGTGAAAGAATGCGAGTACTTGATCGGCCAGCACGTGACTGCAG
  CACTGTATGGGGTGGTCAATGTCAAGAAGGTGTTACAGAGAATGGTGGGAAACCTGTTAAGCCTGGGGAA
  GCGGGAAGGCATCGACCAGTCATACCAGCTCCTGAGAGACTCGCTGGATCTCTATCTGGCAATGTACCCG
  GACCAGGTGCAGCTTCTCCTCCTCCAAGCCAGGCTTTACTTCCACCTGGGAATCTGGCCAGAGAAGGTGC
  TTGACATCCTCCAGCACATCCAAACCCTAGACCCGGGGCAGCACGGGGCGGTGGGCTACCTGGTGCAGCA
  CACTCTAGAGCACATTGAGCGCAAAAAGGAGGAGGTGGGCGTAGAGGTGAAGCTGCGCTCCGATGAGAAG
  CACAGAGATGTCTGCTACTCCATCGGGCTCATTATGAAGCATAAGAGGTATGGCTATAACTGTGTGATCT
  ACGGCTGGGACCCCACCTGCATGATGGGACACGAGTGGATCCGGAACATGAACGTCCACAGCCTGCCGCA
  CGGCCACCACCAGCCTTTCTATAACGTGCTGGTGGAGGACGGCTCCTGTCGATACGCAGCCCAAGAAAAC
  TTGGAATATAACGTGGAGCCTCAAGAAATCTCACACCCTGACGTGGGACGCTATTTCTCAGAGTTTACTG
  GCACTCACTACATCCCAAACGCAGAGCTGGAGATCCGGTATCCAGAAGATCTGGAGTTTGTCTATGAAAC
  GGTGCAGAATATTTACAGTGCAAAGAAAGAGAACATAGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217210 representing NM_015002
  Red=Cloning site Green=Tags(s)
  
  MAAAAVDSAMEVVPALAEEAAPEVAGLSCLVNLPGEVLEYILCCGSLTAADIGRVSSTCRRLRELCQSSG
  KVWKEQFRVRWPSLMKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGP
  EIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQYCNPLS
  DISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDAMNYVLYDQLKFKGNRM
  DYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNFPSHFLLRWCQGAEGATLDIFDYIYID
  AFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKVLQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYP
  DQVQLLLLQARLYFHLGIWPEKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEK
  HRDVCYSIGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAAQEN
  LEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKENIDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015002
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015002.1, NM_015002.2, NP_055817.1
• RefSeq Size: 4227 bp
• RefSeq ORF: 1866 bp
• Locus ID: 23014
• Domains: F-box
• MW: 71.3 kDa
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]