AF10 (MLLT10) (NM_004641) Human Tagged ORF Clone

CAT#: RC217295

MLLT10 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 10 (MLLT10), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417852) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MLLT10
• Synonyms: AF10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217295 representing NM_004641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTCTAGCGACCGGCCCGTGTCACTGGAGGACGAGGTCTCCCATAGTATGAAGGAGATGATTGGAG
  GCTGTTGCGTTTGCTCAGACGAGAGAGGCTGGGCCGAGAACCCGCTGGTTTATTGCGACGGGCACGGCTG
  CAGCGTCGCGGTGCATCAAGCTTGCTATGGCATTGTTCAAGTACCCACTGGACCGTGGTTTTGCAGGAAA
  TGTGAATCTCAGGAGAGAGCAGCCAGAGTGAGATGTGAACTTTGTCCCCATAAGGATGGAGCTTTAAAAA
  GAACAGATAATGGGGGTTGGGCCCATGTGGTTTGTGCCCTGTATATTCCAGAGGTACAATTTGCCAATGT
  TTCCACAATGGAACCAATTGTTTTACAGTCTGTTCCGCATGATCGTTATAATAAGACTTGCTACATTTGT
  GATGAACAAGGAAGAGAAAGCAAAGCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGAC
  AGGCTTTCCATGTAACATGCGCTCAGTTTGCCGGACTGCTTTGTGAAGAAGAAGGTAATGGTGCCGATAA
  TGTCCAATACTGTGGCTACTGTAAATACCATTTTAGTAAGCTGAAAAAGAGCAAACGGGGATCTAATAGG
  TCATATGATCAAAGTTTAAGTGATTCTTCCTCTCACTCTCAGGATAAACATCATGAGAAAGAGAAAAAAA
  AATATAAAGAGAAGGACAAACACAAACAGAAACACAAGAAGCAGCCAGAACCATCACCTGCATTGGTTCC
  ATCCTTGACTGTTACTACAGAAAAAACTTATACAAGCACTAGCAACAACTCTATATCTGGATCATTGAAG
  CGCTTGGAAGATACTACTGCACGATTTACAAATGCAAATTTCCAGGAAGTCTCTGCACACACCTCTAGTG
  GAAAAGATGTTTCAGAGACTAGAGGGTCAGAGGGCAAAGGGAAGAAATCTTCAGCTCACAGCTCAGGTCA
  AAGGGGAAGAAAGCCTGGTGGTGGAAGAAATCCAGGAACAACTGTGTCAGCAGCTAGCCCTTTTCCTCAA
  GGCAGTTTTTCAGGAACTCCAGGCAGTGTAAAGTCATCTTCTGGAAGTTCAGTGCAGTCTCCCCAGGATT
  TCCTGAGCTTTACAGACTCAGATCTGCGTAATGACAGTTACTCTCACTCCCAACAGTCATCAGCAACCAA
  AGATGTACATAAAGGAGAGTCTGGAAGCCAGGAAGGGGGGGTAAATAGTTTTAGTACCTTAATTGGCCTC
  CCTTCAACCTCAGCTGTTACTTCACAGCCTAAAAGCTTTGAAAATTCACCTGGAGATTTGGGTAATTCCA
  GCCTTCCTACAGCAGGATATAAGCGGGCTCAAACTTCTGGCATAGAAGAAGAAACTGTAAAGGAAAAGAA
  AAGGAAAGGAAATAAACAAAGTAAGCATGGGCCTGGCAGACCCAAAGGAAACAAAAATCAAGAGAATGTT
  TCTCATCTCTCAGTTTCTTCTGCTTCACCAACATCATCTGTAGCATCAGCTGCAGGAAGCATAACAAGCT
  CTAGTCTGCAGAAATCTCCTACATTGCTCAGGAATGGAAGTTTACAGAGCCTCAGTGTTGGCTCATCTCC
  AGTTGGTTCAGAAATTTCCATGCAGTATCGGCATGATGGAGCTTGCCCAACAACTACGTTCTCAGAGTTG
  CTGAATGCAATACACAACGACAGAGGTGACAGTTCTACACTAACAAAGCAAGAACTTAAATTCATAGGTA
  TTTATAACAGCAATGATGTAGCAGTATCGTTTCCAAATGTAGTATCTGGCTCGGGATCTAGTACTCCTGT
  CTCCAGCTCTCACTTACCTCAGCAGTCTTCTGGGCATTTGCAACAAGTAGGAGCGCTCTCTCCCTCAGCT
  GTGTCATCTGCAGCCCCTGCTGTTGCTACAACTCAGGCAAATACTCTATCTGGATCTTCTCTCAGTCAGG
  CACCATCTCATATGTATGGCAATAGATCAAATTCATCAATGGCAGCTCTTATAGCTCAGTCTGAAAACAA
  TCAAACAGATCAAGATCTTGGAGACAATAGCCGCAACCTAGTTGGCAGAGGAAGCTCACCCCGAGGAAGT
  CTCTCGCCACGATCCCCTGTAAGCAGCTTACAGATTCGCTATGATCAACCAGGCAACAGCAGTTTGGAAA
  ATCTGCCTCCAGTAGCAGCCAGCATAGAACAGCTTTTGGAGAGGCAGTGGAGTGAAGGACAGCAATTTTT
  ACTAGAACAGGGTACTCCTAGTGACATTTTAGGAATGCTGAAGTCATTACACCAACTTCAAGTTGAAAAC
  CGAAGATTAGAGGAACAAATTAAAAACTTGACTGCCAAAAAGGAACGGCTTCAGTTATTGAATGCACAGC
  TTTCAGTGCCTTTTCCAACAATAACAGCAAATCCTAGTCCGTCTCATCAAATACACACATTTTCAGCACA
  GACTGCTCCTACTACTGATTCCTTGAACAGCAGTAAGAGCCCTCATATAGGAAACAGCTTTTTACCTGAT
  AATTCTCTTCCTGTATTAAATCAGGACTTAACCTCCAGTGGACAAAGTACCAGCAGCTCATCAGCTCTTT
  CTACCCCACCTCCTGCTGGGCAGAGTCCGGCTCAACAAGGCTCAGGAGTGAGTGGAGTTCAGCAGGTCAA
  TGGCGTGACAGTGGGGGCACTAGCTAGTGGAATGCAGCCTGTAACTTCCACCATTCCTGCCGTGTCTGCA
  GTGGGTGGAATAATTGGAGCTTTGCCAGGTAACCAACTGGCAATTAATGGCATTGTAGGAGCTTTAAATG
  GGGTTATGCAGACTCCTGTCACAATGTCCCAGAACCCTACCCCTCTCACCCACACAACCGTACCACCTAA
  TGCAACACATCCAATGCCAGCTACACTGACTAACAGTGCCTCAGGACTAGGATTACTTTCTGACCAGCAA
  CGACAAATACTTATTCATCAACAGCAGTTTCAGCAGTTGTTAAATTCTCAACAGCTCACACCAGTACACA
  GGCACCCCCACTTCACACAGCTACCACCAACCCATTTCTCACCATCCATGGAGATAATGCAAGTCAGAAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217295 representing NM_004641
  Red=Cloning site Green=Tags(s)
  
  MVSSDRPVSLEDEVSHSMKEMIGGCCVCSDERGWAENPLVYCDGHGCSVAVHQACYGIVQVPTGPWFCRK
  CESQERAARVRCELCPHKDGALKRTDNGGWAHVVCALYIPEVQFANVSTMEPIVLQSVPHDRYNKTCYIC
  DEQGRESKAATGACMTCNKHGCRQAFHVTCAQFAGLLCEEEGNGADNVQYCGYCKYHFSKLKKSKRGSNR
  SYDQSLSDSSSHSQDKHHEKEKKKYKEKDKHKQKHKKQPEPSPALVPSLTVTTEKTYTSTSNNSISGSLK
  RLEDTTARFTNANFQEVSAHTSSGKDVSETRGSEGKGKKSSAHSSGQRGRKPGGGRNPGTTVSAASPFPQ
  GSFSGTPGSVKSSSGSSVQSPQDFLSFTDSDLRNDSYSHSQQSSATKDVHKGESGSQEGGVNSFSTLIGL
  PSTSAVTSQPKSFENSPGDLGNSSLPTAGYKRAQTSGIEEETVKEKKRKGNKQSKHGPGRPKGNKNQENV
  SHLSVSSASPTSSVASAAGSITSSSLQKSPTLLRNGSLQSLSVGSSPVGSEISMQYRHDGACPTTTFSEL
  LNAIHNDRGDSSTLTKQELKFIGIYNSNDVAVSFPNVVSGSGSSTPVSSSHLPQQSSGHLQQVGALSPSA
  VSSAAPAVATTQANTLSGSSLSQAPSHMYGNRSNSSMAALIAQSENNQTDQDLGDNSRNLVGRGSSPRGS
  LSPRSPVSSLQIRYDQPGNSSLENLPPVAASIEQLLERQWSEGQQFLLEQGTPSDILGMLKSLHQLQVEN
  RRLEEQIKNLTAKKERLQLLNAQLSVPFPTITANPSPSHQIHTFSAQTAPTTDSLNSSKSPHIGNSFLPD
  NSLPVLNQDLTSSGQSTSSSSALSTPPPAGQSPAQQGSGVSGVQQVNGVTVGALASGMQPVTSTIPAVSA
  VGGIIGALPGNQLAINGIVGALNGVMQTPVTMSQNPTPLTHTTVPPNATHPMPATLTNSASGLGLLSDQQ
  RQILIHQQQFQQLLNSQQLTPVHRHPHFTQLPPTHFSPSMEIMQVRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004641
• ORF Size: 3081 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004641.1, NM_004641.2, NM_004641.3, NP_004632.1
• RefSeq Size: 5140 bp
• RefSeq ORF: 3084 bp
• Locus ID: 8028
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• MW: 108.8 kDa
• Gene Summary: This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]