UBE4A (NM_004788) Human Tagged ORF Clone

CAT#: RC217407

UBE4A (Myc-DDK-tagged)-Human ubiquitination factor E4A (UBE4A), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417750) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217407 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UBE4A protein (Cat# TP317407). The protein was produced from HEK293T cells transfected with UBE4A cDNA clone (Cat# RC217407) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBE4A
• Synonyms: E4; UBOX2; UFD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217407 representing NM_004788
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTG
  ATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCC
  AGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGC
  TCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTA
  TTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGA
  AGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCT
  CGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTG
  CTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGA
  GATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAATACC
  CGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT
  TAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAAGATGTAACTGAGTTTCTGGA
  AGAGGTCATTGAAGCCTTGATATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTT
  GATATTTTATTGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTC
  TTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTAC
  CAATGGGCAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCG
  GGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGG
  AGGCCAACATCCATCAGTTCATGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCA
  GCTCTCTCCAGAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGC
  ACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGA
  ATCTGGGTGCTGCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTT
  TAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGA
  GGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTG
  TAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGT
  AAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCT
  GCTGACAATCTTCGTGAGCAGTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAG
  AGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCAT
  AGGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTAT
  GTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGATGACATTT
  TGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGCATAGAAAG
  AATGAAGAATCCCCACCTGAGGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAG
  ACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCC
  AACTTGCAGAGGCTCTAATCAAGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACA
  GAAGTTTAATTACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATACCTATCGGGAG
  AGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCACTTTTCCTCCGCT
  TTCTTAACCTGCTAATGAATGATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAA
  GATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAG
  GCTGGCCTACAGATGTTTGGACAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCC
  TTGCCTTTCTCACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCAT
  GTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAA
  TTTGACTTCAAACCCCAGCAGCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGA
  ATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGT
  CTTGAAGAAAATAAATAAGCCTGGGAATATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCT
  CTTGCAGACCTCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTA
  TGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGC
  AAGACATTTGCTCAGTGACCAAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCA
  AACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217407 representing NM_004788
  Red=Cloning site Green=Tags(s)
  
  MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISR
  SFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFA
  RLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNT
  RTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLEEVIEALILDEEVRTFPEVMIPVF
  DILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTP
  GVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGR
  TKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMR
  GLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPA
  ADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAY
  VPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQ
  TPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRE
  SIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKE
  AGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSE
  FDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKS
  LADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRP
  NTELKEKIQRWLAERKQQKEQLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004788
• ORF Size: 3219 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004788.1, NM_004788.2, NM_004788.3, NP_004779.2
• RefSeq Size: 6138
• RefSeq ORF: 3222
• Locus ID: 9354
• Domains: U-box
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 123.3 kDa
• Gene Summary: This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]