HFE (NM_000410) Human Tagged ORF Clone
CAT#: RC217447L2
- LentiORF®
Lenti ORF clone of Human hemochromatosis (HFE), transcript variant 1, mGFP tagged
"NM_000410" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | HFE |
Synonyms | HFE1; HH; HLA-H; MVCD7; TFQTL2 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC217447).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000410 |
ORF Size | 1044 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000410.2, NP_000401.1 |
RefSeq Size | 2727 bp |
RefSeq ORF | 1047 bp |
Locus ID | 3077 |
Cytogenetics | 6p22.2 |
Domains | MHC_I, ig, IGc1 |
Protein Families | Druggable Genome, Transmembrane |
MW | 40.11 kDa |
Gene Summary | 'The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119914 | HFE (untagged)-Human hemochromatosis (HFE), transcript variant 1 |
USD 760.00 |
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RC217447 | HFE (Myc-DDK-tagged)-Human hemochromatosis (HFE), transcript variant 1 |
USD 420.00 |
|
RG217447 | HFE (GFP-tagged) - Human hemochromatosis (HFE), transcript variant 1 |
USD 460.00 |
|
RC217447L1 | Lenti ORF clone of Human hemochromatosis (HFE), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC217447L3 | Lenti ORF clone of Human hemochromatosis (HFE), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC217447L4 | Lenti ORF clone of Human hemochromatosis (HFE), transcript variant 1, mGFP tagged |
USD 620.00 |
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