ZNF521 (NM_015461) Human Tagged ORF Clone

CAT#: RC217463

ZNF521 (Myc-DDK-tagged)-Human zinc finger protein 521 (ZNF521)

Product Images

Western blot validation of overexpression lysate (Cat# LY402451) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217463 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF521
• Synonyms: EHZF; Evi3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217463 representing NM_015461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGACTG
  AAGATGGAGAGGCACTAGATTGTAAGAAGAGGCCGGAAGACGGGGAGGAGTTGGAAGACGAAGCTGTGCA
  CAGCTGTGACAGCTGCCTCCAGGTGTTTGAATCGCTGAGCGATATCACAGAACACAAGATTAATCAATGT
  CAACTGACAGATGGAGTGGATGTTGAAGATGATCCGACTTGCTCTTGGCCAGCTTCCTCACCTTCTAGCA
  AGGATCAGACTTCCCCTAGCCATGGAGAAGGTTGCGATTTTGGAGAGGAAGAAGGTGGCCCTGGGCTTCC
  ATACCCGTGTCAATTCTGTGACAAGTCGTTTAGCCGCCTCAGCTACCTAAAGCACCATGAGCAGAGTCAC
  AGTGACAAACTGCCTTTCAAATGCACCTACTGCAGTAGGCTGTTCAAACACAAGCGCAGCCGAGATCGCC
  ACATAAAACTCCACACCGGGGACAAGAAGTACCACTGCAGTGAATGTGATGCTGCGTTTTCCAGAAGTGA
  TCACTTGAAGATCCACTTAAAGACTCACACGTCCAACAAGCCATATAAATGTGCCATTTGTCGCCGTGGG
  TTTCTGTCCTCTAGTTCCTTACACGGACACATGCAGGTTCATGAGAGGAACAAGGACGGCTCTCAGTCCG
  GTTCCAGGATGGAGGACTGGAAGATGAAGGACACTCAGAAGTGCAGTCAGTGTGAGGAAGGCTTTGACTT
  CCCGGAAGACCTCCAAAAACACATTGCAGAGTGCCACCCCGAATGCTCCCCAAATGAGGACCGAGCGGCC
  CTCCAGTGTGTCTACTGCCACGAGCTCTTCGTAGAGGAGACCTCCCTCATGAACCACATGGAGCAGGTGC
  ATAGCGGGGAGAAGAAGAACTCATGCAGCATTTGTTCTGAGAGTTTCCACACAGTTGAGGAACTGTACAG
  CCACATGGACAGTCACCAGCAACCGGAGTCATGCAATCACAGCAACAGCCCTTCCCTGGTCACGGTGGGC
  TATACCTCCGTGTCCAGTACGACTCCAGATTCCAACCTCTCAGTGGACAGCTCAACCATGGTGGAAGCTG
  CCCCGCCAATCCCAAAGAGTCGAGGGAGGAAGAGGGCCGCTCAACAAACCCCTGACATGACTGGTCCCTC
  GAGTAAACAAGCAAAAGTTACCTACAGCTGTATTTACTGCAACAAACAATTATTTTCAAGTCTTGCAGTT
  CTGCAGATTCACCTGAAAACTATGCACTTAGATAAGCCAGAACAGGCCCATATTTGTCAGTATTGCTTGG
  AGGTCCTGCCCTCACTCTATAACCTAAATGAACATCTTAAGCAAGTGCATGAAGCTCAGGACCCAGGTCT
  GATTGTTTCTGCCATGCCTGCCATTGTCTACCAGTGTAACTTCTGTTCCGAAGTTGTCAACGACCTCAAC
  ACTCTTCAGGAACACATCCGATGTTCTCATGGATTTGCAAACCCTGCAGCTAAAGATAGTAATGCATTCT
  TTTGTCCCCATTGCTATATGGGGTTTCTCACTGACTCTTCCCTGGAAGAGCATATTAGACAGGTTCATTG
  TGACCTCAGTGGCTCCCGATTTGGGTCTCCAGTGCTTGGGACTCCCAAAGAACCAGTAGTAGAAGTCTAT
  TCTTGTTCCTATTGTACAAATTCGCCAATATTCAACAGCGTTCTTAAACTGAACAAGCATATCAAAGAGA
  ATCATAAAAACATTCCCTTGGCCCTGAATTATATCCACAATGGGAAGAAATCCAGGGCCTTAAGCCCCCT
  ATCTCCTGTGGCCATAGAGCAGACATCTCTTAAGATGATGCAGGCAGTAGGAGGTGCACCTGCACGTCCC
  ACTGGAGAATATATCTGTAATCAATGTGGTGCTAAGTACACATCCCTAGACAGCTTTCAGACTCACCTAA
  AAACTCATCTCGACACTGTGCTTCCAAAATTGACCTGTCCTCAGTGCAACAAGGAATTCCCCAACCAAGA
  ATCCTTGCTGAAGCATGTTACCATTCACTTTATGATCACTTCAACGTATTACATCTGTGAGAGTTGTGAC
  AAGCAATTCACATCAGTGGATGACCTTCAGAAACACCTGCTGGACATGCACACCTTTGTCTTCTTTCGCT
  GCACCCTCTGCCAGGAAGTTTTTGACTCAAAAGTCTCCATTCAGCTCCACTTGGCTGTGAAGCACAGTAA
  CGAAAAGAAAGTCTATAGGTGCACATCTTGCAACTGGGACTTCCGCAACGAAACTGACTTGCAGCTCCAT
  GTGAAACACAACCACCTGGAAAACCAAGGGAAAGTGCATAAGTGCATTTTCTGCGGTGAGTCCTTTGGCA
  CCGAGGTGGAGCTGCAATGCCACATCACCACTCACAGTAAGAAGTACAACTGCAAGTTCTGTAGCAAAGC
  CTTCCATGCGATCATTTTGTTAGAAAAACACTTGCGAGAAAAACACTGTGTATTCGAAACCAAGACACCC
  AACTGTGGAACAAATGGAGCTTCCGAGCAAGTGCAGAAAGAGGAAGTGGAGCTGCAGACTTTGCTGACCA
  ACAGCCAGGAGTCCCACAACAGTCACGATGGGAGCGAAGAAGACGTTGACACCTCTGAGCCTATGTACGG
  CTGCGACATTTGTGGGGCAGCCTACACTATGGAAACTTTGCTGCAGAATCACCAGCTCCGAGACCACAAC
  ATCAGACCTGGAGAAAGTGCCATCGTGAAAAAGAAAGCTGAGCTCATTAAAGGGAATTACAAGTGCAACG
  TGTGCTCTCGAACCTTCTTCTCCGAAAATGGCCTCCGGGAACATATGCAGACCCACCTAGGCCCTGTCAA
  ACACTACATGTGCCCTATTTGCGGAGAGCGGTTTCCCTCCCTTTTAACTCTTACTGAACACAAAGTCACG
  CATAGTAAGAGTCTTGATACTGGAAACTGCCGGATTTGCAAGATGCCTCTCCAGAGTGAAGAGGAGTTTT
  TAGAGCATTGCCAAATGCACCCTGACTTGAGGAATTCCCTGACAGGCTTTCGCTGCGTGGTGTGCATGCA
  GACAGTGACCTCCACCTTGGAACTCAAAATCCATGGGACGTTCCACATGCAAAAGACAGGGAATGGGTCT
  GCAGTTCAGACCACAGGGCGGGGCCAGCACGTCCAAAAACTGTATAAGTGCGCATCTTGCCTCAAAGAAT
  TCCGTTCCAAGCAAGATCTGGTGAAACTTGATATCAATGGCCTGCCATATGGTCTGTGTGCCGGCTGCGT
  GAATCTCAGTAAGAGCGCCAGCCCAGGCATTAACGTCCCTCCCGGCACGAATAGACCAGGCTTGGGCCAG
  AATGAGAATCTGAGTGCCATTGAGGGGAAAGGCAAGGTGGGGGGACTGAAGACACGCTGCTCTAGCTGCA
  ACGTTAAGTTTGAGTCTGAAAGTGAACTCCAGAACCACATCCAAACCATCCACCGAGAGCTCGTGCCAGA
  CAGCAACAGCACACAGTTGAAAACGCCCCAAGTATCACCAATGCCCAGAATCAGTCCCTCCCAGTCGGAT
  GAGAAGAAGACCTATCAATGCATCAAGTGTCAGATGGTTTTCTACAATGAATGGGATATTCAGGTTCATG
  TTGCAAATCACATGATTGATGAAGGACTGAACCATGAATGCAAACTCTGCAGCCAGACCTTTGACTCTCC
  TGCCAAACTCCAGTGCCACCTGATAGAGCACAGCTTCGAAGGGATGGGAGGCACCTTCAAGTGTCCAGTC
  TGCTTTACAGTATTTGTTCAAGCAAACAAGTTGCAGCAGCATATTTTCTCTGCCCATGGACAAGAAGACA
  AGATCTATGACTGTACACAATGTCCACAGAAGTTTTTCTTCCAAACAGAGCTGCAGAATCATACAATGAC
  CCAACACAGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217463 representing NM_015461
  Red=Cloning site Green=Tags(s)
  
  MSRRKQAKPRSLKDPNCKLEDKTEDGEALDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQC
  QLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSH
  SDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRG
  FLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAA
  LQCVYCHELFVEETSLMNHMEQVHSGEKKNSCSICSESFHTVEELYSHMDSHQQPESCNHSNSPSLVTVG
  YTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTGPSSKQAKVTYSCIYCNKQLFSSLAV
  LQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLN
  TLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVY
  SCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARP
  TGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCD
  KQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLH
  VKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTP
  NCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHN
  IRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVT
  HSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGS
  AVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGINVPPGTNRPGLGQ
  NENLSAIEGKGKVGGLKTRCSSCNVKFESESELQNHIQTIHRELVPDSNSTQLKTPQVSPMPRISPSQSD
  EKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPV
  CFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015461
• ORF Size: 3933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015461.1, NM_015461.2, NP_056276.1
• RefSeq Size: 4869 bp
• RefSeq ORF: 3936 bp
• Locus ID: 25925
• Domains: zf-C2H2
• MW: 147.7 kDa