CDADC1 (NM_030911) Human Tagged ORF Clone

CAT#: RC217484

CDADC1 (Myc-DDK-tagged)-Human cytidine and dCMP deaminase domain containing 1 (CDADC1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410668) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217484 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CDADC1 protein (Cat# TP317484). The protein was produced from HEK293T cells transfected with CDADC1 cDNA clone (Cat# RC217484) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDADC1
• Synonyms: bA103J18.1; NYD-SP15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGAAGCTGGGCAGATGCAAAATCTGGAGAGCGCGAGGGCCGGGCGGTCAGTCAGCACCCAGACTG
  GCAGCATGACCGGTCAGATACCAAGGCTTTCTAAAGTCAACCTTTTCACTCTGCTCAGCCTCTGGATGGA
  GCTCTTTCCAGCAGAAGCCCAGCGGCAAAAATCTCAGAAAAATGAAGAGGGAAAGCATGGACCCTTAGGA
  GATAATGAAGAGAGGACCAGAGTATCTACTGACAAAAGACAGGTAAAGAGAACTGGTCTTGTGGTGGTGA
  AAAACATGAAAATTGTTGGTCTCCACTGTTCTAGTGAAGATTTACATGCCGGGCAGATTGCTCTTATTAA
  ACATGGGTCAAGGCTGAAAAACTGTGATCTTTATTTTTCCAGAAAACCATGTTCTGCTTGTTTGAAAATG
  ATTGTAAATGCTGGAGTTAACCGAATTTCATACTGGCCTGCTGATCCAGAAATAAGTTTGCTTACGGAGG
  CTTCTAGTTCTGAAGATGCAAAGTTAGATGCCAAAGCAGTGGAAAGATTGAAGTCAAACAGTCGGGCCCA
  TGTGTGTGTCTTACTTCAACCTTTGGTGTGTTATATGGTGCAGTTTGTAGAGGAGACCTCTTACAAATGT
  GACTTTATTCAAAAAATTACAAAAACATTGCCGGATGCTAACACTGACTTTTATTATGAATGTAAACAAG
  AAAGAATAAAAGAATATGAAATGTTATTTTTGGTTTCAAATGAAGAAATGCATAAGCAAATACTGATGAC
  TATAGGTTTGGAGAACCTGTGTGAAAATCCATACTTTAGCAATCTAAGGCAAAACATGAAAGACCTTATC
  CTACTTTTGGCCACAGTAGCTTCCAGTGTGCCGAACTTTAAACACTTCGGATTTTACCGTAGCAATCCAG
  AACAGATTAATGAAATTCACAATCAAAGTTTGCCACAGGAAATTGCAAGGCACTGCATGGTTCAGGCCAG
  GTTATTGGCATATCGAACTGAGGATCATAAAACAGGAGTTGGGGCAGTCATTTGGGCAGAAGGGAAATCT
  AGAAGTTGTGATGGAACAGGTGCCATGTACTTTGTAGGATGTGGTTACAATGCTTTTCCTGTTGGATCTG
  AGTATGCTGACTTCCCACACATGGATGACAAGCAGAAAGACAGAGAAATAAGGAAATTCAGATACATCAT
  ACATGCGGAACAGAATGCCTTGACATTTAGGTGTCAAGAAATAAAACCAGAAGAAAGAAGCATGATTTTT
  GTGACAAAGTGCCCATGTGATGAGTGTGTACCTTTAATTAAAGGTGCAGGCATAAAACAAATCTATGCAG
  GAGATGTAGATGTTGGAAAAAAGAAGGCAGACATCTCTTACATGAGGTTCGGGGAGCTTGAAGGTGTTAG
  CAAATTTACGTGGCAGCTGAATCCATCAGGAGCTTATGGTCTTGAACAAAATGAGCCTGAAAGGAGAGAA
  AATGGTGTGTTGAGACCTGTCCCACAGAAGGAAGAGCAGCACCAGGACAAGAAGCTGCGCCTCGGAATCC
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEAGQMQNLESARAGRSVSTQTGSMTGQIPRLSKVNLFTLLSLWMELFPAEAQRQKSQKNEEGKHGPLG
  DNEERTRVSTDKRQVKRTGLVVVKNMKIVGLHCSSEDLHAGQIALIKHGSRLKNCDLYFSRKPCSACLKM
  IVNAGVNRISYWPADPEISLLTEASSSEDAKLDAKAVERLKSNSRAHVCVLLQPLVCYMVQFVEETSYKC
  DFIQKITKTLPDANTDFYYECKQERIKEYEMLFLVSNEEMHKQILMTIGLENLCENPYFSNLRQNMKDLI
  LLLATVASSVPNFKHFGFYRSNPEQINEIHNQSLPQEIARHCMVQARLLAYRTEDHKTGVGAVIWAEGKS
  RSCDGTGAMYFVGCGYNAFPVGSEYADFPHMDDKQKDREIRKFRYIIHAEQNALTFRCQEIKPEERSMIF
  VTKCPCDECVPLIKGAGIKQIYAGDVDVGKKKADISYMRFGELEGVSKFTWQLNPSGAYGLEQNEPERRE
  NGVLRPVPQKEEQHQDKKLRLGIH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030911
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030911.1, NM_030911.2, NM_030911.3, NP_112173.1
• RefSeq Size: 3417 bp
• RefSeq ORF: 1545 bp
• Locus ID: 81602
• Domains: dCMP_cyt_deam
• MW: 58.5 kDa