SVH (ARMC10) (NM_031905) Human Tagged ORF Clone

CAT#: RC217510

ARMC10 (Myc-DDK-tagged)-Human armadillo repeat containing 10 (ARMC10), transcript variant A

Product Images

Western blot validation of overexpression lysate (Cat# LY410452) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217510 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARMC10 protein (Cat# TP317510). The protein was produced from HEK293T cells transfected with ARMC10 cDNA clone (Cat# RC217510) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMC10
• Synonyms: PNAS-112; PNAS112; PSEC0198; SVH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217510 representing NM_031905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGCCCCCGGGGCGCGGGCTGGGTGGCGGCGGGCCTGCTGCTCGGCGCGGGCGCCTGCTACTGCA
  TTTACAGGCTGACCCGGGGTCGGCGGCGGGGCGACCGCGAGCTCGGGATACGCTCTTCGAAGTCCGCAGG
  TGCCCTGGAAGAAGGGACGTCAGAGGGTCAGTTGTGCGGGCGCTCGGCCCGGCCTCAGACGGGAGGTACC
  TGGGAGTCACAGTGGTCCAAGACCTCGCAGCCTGAAGACTTAACTGATGGTTCATATGATGATGTTCTAA
  ATGCTGAACAACTTCAGAAACTCCTTTACCTGCTGGAGTCAACGGAGGATCCTGTAATTATTGAAAGAGC
  TTTGATTACTTTGGGTAACAATGCAGCCTTTTCAGTTAACCAAGCTATTATTCGTGAATTGGGTGGTATT
  CCAATTGTTGCAAACAAAATCAACCATTCCAACCAGAGTATTAAAGAGAAAGCTTTAAATGCACTAAATA
  ACCTGAGTGTGAATGTTGAAAATCAAATCAAGATAAAGATATACATCAGTCAAGTATGTGAGGATGTCTT
  CTCTGGTCCTCTGAACTCTGCTGTGCAGCTGGCTGGACTGACATTGTTGACAAACATGACTGTTACCAAT
  GACCACCAGCACATGCTTCACAGTTACATTACAGACCTGTTCCAGGTGTTACTTACTGGAAATGGAAACA
  CGAAGGTGCAAGTTTTGAAACTGCTTTTGAATTTGTCTGAAAATCCAGCCATGACAGAAGGACTTCTCCG
  TGCCCAAGTGGATTCATCATTCCTTTCCCTTTATGACAGCCACGTAGCAAAGGAGATTCTTCTTCGAGTA
  CTTACGCTATTTCAGAATATAAAGAACTGCCTCAAAATAGAAGGCCATTTAGCTGTGCAGCCTACTTTCA
  CTGAAGGTTCATTGTTTTTCCTGTTACATGGAGAAGAATGTGCCCAGAAAATAAGAGCTTTAGTTGATCA
  CCATGATGCAGAGGTGAAGGAAAAGGTTGTAACAATAATACCCAAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217510 representing NM_031905
  Red=Cloning site Green=Tags(s)
  
  MGGPRGAGWVAAGLLLGAGACYCIYRLTRGRRRGDRELGIRSSKSAGALEEGTSEGQLCGRSARPQTGGT
  WESQWSKTSQPEDLTDGSYDDVLNAEQLQKLLYLLESTEDPVIIERALITLGNNAAFSVNQAIIRELGGI
  PIVANKINHSNQSIKEKALNALNNLSVNVENQIKIKIYISQVCEDVFSGPLNSAVQLAGLTLLTNMTVTN
  DHQHMLHSYITDLFQVLLTGNGNTKVQVLKLLLNLSENPAMTEGLLRAQVDSSFLSLYDSHVAKEILLRV
  LTLFQNIKNCLKIEGHLAVQPTFTEGSLFFLLHGEECAQKIRALVDHHDAEVKEKVVTIIPKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031905
• ORF Size: 1029 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031905.2, NM_031905.3, NM_031905.4, NP_114111.2
• RefSeq Size: 2629
• RefSeq ORF: 1032
• Locus ID: 83787
• Domains: DUF634
• Protein Families: Transmembrane
• MW: 37.4 kDa
• Gene Summary: This gene encodes a protein that contains an armadillo repeat and transmembrane domain. The encoded protein decreases the transcriptional activity of the tumor suppressor protein p53 through direct interaction with the DNA-binding domain of p53, and may play a role in cell growth and survival. Upregulation of this gene may play a role in hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Sep 2011]