Argininosuccinate Lyase (ASL) (NM_001024944) Human Tagged ORF Clone
CAT#: RC217587L4
- LentiORF®
Lenti-ORF clone of ASL (mGFP-tagged)-Human argininosuccinate lyase (ASL), transcript variant 3
"NM_001024944" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | ASL |
Synonyms | ASAL |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC217587).
|
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
ACCN | NM_001024944 |
ORF Size | 1332 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024944.1, NP_001020115.1 |
RefSeq Size | 2001 bp |
RefSeq ORF | 1335 bp |
Locus ID | 435 |
Cytogenetics | 7q11.21 |
Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways |
MW | 49.3 kDa |
Gene Summary | 'This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302322 | ASL (untagged)-Human argininosuccinate lyase (ASL), transcript variant 3 |
USD 750.00 |
|
RC217587 | ASL (Myc-DDK-tagged)-Human argininosuccinate lyase (ASL), transcript variant 3 |
USD 420.00 |
|
RG217587 | ASL (GFP-tagged) - Human argininosuccinate lyase (ASL), transcript variant 3 |
USD 460.00 |
|
RC217587L3 | Lenti-ORF clone of ASL (Myc-DDK-tagged)-Human argininosuccinate lyase (ASL), transcript variant 3 |
USD 620.00 |
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