TICAM2 (NM_021649) Human Tagged ORF Clone

CAT#: RC217645

TICAM2 (Myc-DDK-tagged)-Human toll-like receptor adaptor molecule 2 (TICAM2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402870) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217645 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TICAM2 protein (Cat# TP317645). The protein was produced from HEK293T cells transfected with TICAM2 cDNA clone (Cat# RC217645) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TICAM2
• Synonyms: MyD88-4; TICAM-2; TIRAP3; TIRP; TRAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217645 representing NM_021649
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTATCGGGAAGTCTAAAATAAATTCCTGCCCTCTTTCTCTCTCTTGGGGTAAAAGGCACAGTGTGG
  ATACAAGTCCAGGATATCATGAGTCAGATTCCAAGAAGTCTGAAGATCTATCCTTGTGTAATGTTGCTGA
  GCACAGCAATACAACAGAGGGGCCAACAGGAAAGCAGGAGGGAGCTCAGAGCGTGGAAGAGATGTTTGAA
  GAAGAAGCTGAAGAAGAGGTGTTCCTCAAATTTGTGATATTGCATGCAGAAGATGACACAGATGAAGCCC
  TCAGAGTCCAGAATCTGCTACAAGATGACTTTGGTATCAAACCCGGAATAATCTTTGCTGAGATGCCATG
  TGGCAGACAGCATTTACAGAATTTAGATGATGCTGTAAATGGGTCTGCATGGACAATCTTATTACTGACT
  GAAAACTTTTTAAGAGATACTTGGTGTAATTTCCAGTTCTATACGTCCCTAATGAACTCCGTTAACAGGC
  AGCATAAATACAACTCTGTTATACCCATGCGGCCCCTGAACAATCCCCTTCCCCGAGAAAGGACTCCCTT
  TGCCCTCCAAACCATCAATGCCTTAGAGGAAGAAAGTCGTGGATTTCCTACACAAGTAGAAAGAATTTTT
  CAGGAGTCTGTGTATAAGACACAACAAACTATATGGAAAGAGACAAGAAATATGGTACAAAGACAATTTA
  TTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217645 representing NM_021649
  Red=Cloning site Green=Tags(s)
  
  MGIGKSKINSCPLSLSWGKRHSVDTSPGYHESDSKKSEDLSLCNVAEHSNTTEGPTGKQEGAQSVEEMFE
  EEAEEEVFLKFVILHAEDDTDEALRVQNLLQDDFGIKPGIIFAEMPCGRQHLQNLDDAVNGSAWTILLLT
  ENFLRDTWCNFQFYTSLMNSVNRQHKYNSVIPMRPLNNPLPRERTPFALQTINALEEESRGFPTQVERIF
  QESVYKTQQTIWKETRNMVQRQFIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021649
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021649.1, NM_021649.2, NM_021649.3, NM_021649.4, NM_021649.5, NM_021649.6, NM_021649.7, NP_067681.1
• RefSeq Size: 3453 bp
• RefSeq ORF: 708 bp
• Locus ID: 353376
• Protein Families: Druggable Genome
• Protein Pathways: Toll-like receptor signaling pathway
• MW: 26.7 kDa
• Gene Summary: TIRP is a Toll/interleukin-1 receptor (IL1R; MIM 147810) (TIR) domain-containing adaptor protein involved in Toll receptor signaling (see TLR4; MIM 603030). [supplied by OMIM, Apr 2004]