RAGEF2 (RAPGEF6) (NM_016340) Human Tagged ORF Clone

CAT#: RC217657

RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY414036) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217657 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RAPGEF6 protein (Cat# TP317657). The protein was produced from HEK293T cells transfected with RAPGEF6 cDNA clone (Cat# RC217657) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAPGEF6
• Synonyms: KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217657 representing NM_016340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGGACTCCCGAGG
  ACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAATCTCAGGGAACATCAGCTTAG
  ATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGCAATCAGGTTCTCTTTTGTTCAGAAACGATT
  GCCAGATGTTGGTATATCCTACTTTCTGGATCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCA
  GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGT
  GGTAGAGAATGCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA
  AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTTAACAGCTATC
  TTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCATCCACAGGTGACTCATGTGTC
  TTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGAAGCAGCAGTTTATCTGATATCTATCAGGCT
  ACGGAGAGTGAGGTAGGAGATGTAGATTTGACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACG
  AAGAGGAAGATGAAGAGATTGATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGA
  AAAAGAACCTGCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA
  TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTGGTAGAGCAGG
  CTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTTATTTTAAACGGCACTGTGGA
  AATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATGGGAAATAGTTTTGGAATTACTCCCACTCTG
  GATAAGCAGTACATGCATGGAATTGTCAGGACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGC
  AAGATTATTGGAGAATTTTAAACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGT
  TATGGTACATGAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA
  CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATAGAAGATTTTC
  TATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAACTATTGGAATGGTTTAAGAT
  CGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTATGGGTAAATAATCATTTTAATGATTTTGAA
  GGTGACCCTGCTATGACTCGATTTCTAGAGGAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTC
  ATCTCCGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTC
  CCGCGAGTCCCCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA
  GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAAGTAAATGGAC
  AAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAATAATACTCATCTTGCACTTAC
  TGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTTAGGACTGAACAAGAGAAATCTGGTGTTCCT
  CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAAC
  AGACATCACAGGAGAAAGGAAGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAG
  GAAGATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA
  TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCCATCCCTGGAA
  CACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTGGATTTTTCCAATCCTTCAGA
  TATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAGCAAAGTTGCTACATTATCATCAGTAAAGAC
  ACCACAGCTAAAGAAGTAGTTTTTCATGCTGTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATT
  CTCTCTGTGAAGTTTCTGTTACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAA
  ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGT
  TCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGTACCATTGAGG
  TGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAACCGACTGAGTACATCGATGA
  CCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTGAAGAGGTTTGAGGACATTGTAAACCAAGAG
  ACATTCTGGGTTGCCTCAGAAATTTTAACTGAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATT
  TTATTAAAATTGCACTTCATTGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTT
  GAACCTGGCATCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTT
  CAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTTAGTAGTCAAA
  GTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTACATGAAGGAAA
  TGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTAAGAATGATTTCCAAGGAAATCCGCCAAGTT
  GTTCGAATGACTTCTGCTAACATGGACCCAGCTATGATGTTTCGACAGAGGTCACTGAGTCAAGGAAGCA
  CAAATTCAAACATGCTGGATGTTCAGGGAGGTGCTCACAAAAAAAGGGCACGCCGCAGCTCTCTGCTTAA
  TGCCAAGAAGCTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAGCAGTATCTTTCCAGTCTCGATGTA
  GAGACAGATGAGGAGAAGTTCCAGATGATGTCATTACAGTGGGAGCCTGCATATGGTACCTTGACCAAGA
  ATTTAAGTGAGAAAAGATCAGCCAAATCATCTGAAATGTCTCCAGTGCCTATGAGGTCAGCTGGCCAAAC
  AACTAAAGCCCACTTGCATCAACCCCACAGAGTAAGCCAGGTGCTTCAGGTGCCAGCTGTTAATTTGCAC
  CCCATCAGGAAGAAGGGACAAACAAAAGACCCTGCACTGAATACAAGTTTACCTCAGAAAGTTTTAGGAA
  CAACTGAAGAAATAAGTGGTAAGAAGCATACAGAAGACACTATTTCTGTGGCGTCATCTTTACATTCTAG
  TCCTCCTGCATCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTTATTCCATCAGCTAAATCTGACAAC
  TTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGGTCCAGCATCGTGAGCAATTGTTCTGTTGACTCCA
  TGTCTGCAGCTCTACAGGATGAACGGTGTTCCTCTCAGGCCCTGGCAGTCCCTGAATCCACTGGGGCATT
  GGAAAAGACAGAGCACGCTTCAGGGATAGGAGATCATAGTCAACATGGCCCTGGGTGGACACTCTTGAAG
  CCATCTCTAATCAAGTGTTTAGCTGTCTCATCGTCTGTGAGCAATGAAGAGATTTCTCAAGAGCATATCA
  TTATAGAAGCAGCTGACAGTGGTCGTGGAAGTTGGACTTCGTGTTCAAGCAGCTCCCATGACAACTTCCA
  AAGCCTTCCAAACCCAAAAAGCTGGGATTTTTTGAACTCTTACAGACATACCCATTTGGATGACCCCATT
  GCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCCTGTTCTAAAAGCTGCTCTAGAACTTGTGGGCAGT
  GTAAAGGAAGCCTAGAGAGAAAGAGTTGGACCTCCTCCAGTTCTCTGTCTGACACGTATGAACCAAACTA
  TGGGACAGTTAAACAGAGAGTATTGGAGAGCACCCCAGCTGAGTCATCTGAAGGCTTGGACCCCAAGGAT
  GCCACTGACCCAGTTTATAAAACTGTCACTTCAAGTACAGAAAAGGGCTTGATTGTGTACTGTGTCACCT
  CACCCAAGAAGGACGATAGGTATAGGGAGCCACCTCCCACTCCTCCAGGATATTTGGGGATTTCTTTAGC
  GGACCTAAAGGAAGGACCCCACACACACCTAAAACCTCCAGATTATAGTGTGGCAGTGCAGAGGTCAAAG
  ATGATGCATAACAGCCTCTCTAGACTGCCACCAGCTTCTCTCAGTAGCAACCTCGTGGCCTGTGTTCCAT
  CGAAGATTGTAACTCAGCCTCAGAGGCATAATTTGCAGCCATTCCATCCTAAACTAGGAGATGTGACTGA
  TGCAGATAGCGAAGCAGATGAAAATGAACAAGTTTCAGCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217657 representing NM_016340
  Red=Cloning site Green=Tags(s)
  
  MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETI
  ARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRR
  RFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA
  TESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPA
  FANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTL
  DKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT
  PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFE
  GDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEG
  VEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVP
  HIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ
  SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKD
  TTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC
  SDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQE
  TFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHL
  QDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQV
  VRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDV
  ETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLH
  PIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDN
  LSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLK
  PSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPI
  AEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKD
  ATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSK
  MMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016340
• ORF Size: 4803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016340.3, NM_016340.4, NM_016340.5, NP_057424.2, NP_057424.3
• RefSeq Size: 6390
• RefSeq ORF: 4806
• Locus ID: 51735
• Domains: RA, cNMP, RasGEFN, PDZ, RasGEF
• MW: 179.2 kDa