RAGEF2 (RAPGEF6) (NM_016340) Human Tagged ORF Clone
CAT#: RC217657
RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2
"NM_016340" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RAPGEF6 |
Synonyms | KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2; RAGEF2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217657 representing NM_016340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGGACTCCCGAGG ACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAATCTCAGGGAACATCAGCTTAG ATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGCAATCAGGTTCTCTTTTGTTCAGAAACGATT GCCAGATGTTGGTATATCCTACTTTCTGGATCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCA GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGT GGTAGAGAATGCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTTAACAGCTATC TTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCATCCACAGGTGACTCATGTGTC TTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGAAGCAGCAGTTTATCTGATATCTATCAGGCT ACGGAGAGTGAGGTAGGAGATGTAGATTTGACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACG AAGAGGAAGATGAAGAGATTGATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGA AAAAGAACCTGCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTGGTAGAGCAGG CTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTTATTTTAAACGGCACTGTGGA AATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATGGGAAATAGTTTTGGAATTACTCCCACTCTG GATAAGCAGTACATGCATGGAATTGTCAGGACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGC AAGATTATTGGAGAATTTTAAACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGT TATGGTACATGAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATAGAAGATTTTC TATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAACTATTGGAATGGTTTAAGAT CGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTATGGGTAAATAATCATTTTAATGATTTTGAA GGTGACCCTGCTATGACTCGATTTCTAGAGGAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTC ATCTCCGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTC CCGCGAGTCCCCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAAGTAAATGGAC AAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAATAATACTCATCTTGCACTTAC TGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTTAGGACTGAACAAGAGAAATCTGGTGTTCCT CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAAC AGACATCACAGGAGAAAGGAAGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAG GAAGATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCCATCCCTGGAA CACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTGGATTTTTCCAATCCTTCAGA TATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAGCAAAGTTGCTACATTATCATCAGTAAAGAC ACCACAGCTAAAGAAGTAGTTTTTCATGCTGTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATT CTCTCTGTGAAGTTTCTGTTACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAA ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGT TCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGTACCATTGAGG TGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAACCGACTGAGTACATCGATGA CCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTGAAGAGGTTTGAGGACATTGTAAACCAAGAG ACATTCTGGGTTGCCTCAGAAATTTTAACTGAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATT TTATTAAAATTGCACTTCATTGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTT GAACCTGGCATCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTT CAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTTAGTAGTCAAA GTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTACATGAAGGAAA TGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTAAGAATGATTTCCAAGGAAATCCGCCAAGTT GTTCGAATGACTTCTGCTAACATGGACCCAGCTATGATGTTTCGACAGAGGTCACTGAGTCAAGGAAGCA CAAATTCAAACATGCTGGATGTTCAGGGAGGTGCTCACAAAAAAAGGGCACGCCGCAGCTCTCTGCTTAA TGCCAAGAAGCTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAGCAGTATCTTTCCAGTCTCGATGTA GAGACAGATGAGGAGAAGTTCCAGATGATGTCATTACAGTGGGAGCCTGCATATGGTACCTTGACCAAGA ATTTAAGTGAGAAAAGATCAGCCAAATCATCTGAAATGTCTCCAGTGCCTATGAGGTCAGCTGGCCAAAC AACTAAAGCCCACTTGCATCAACCCCACAGAGTAAGCCAGGTGCTTCAGGTGCCAGCTGTTAATTTGCAC CCCATCAGGAAGAAGGGACAAACAAAAGACCCTGCACTGAATACAAGTTTACCTCAGAAAGTTTTAGGAA CAACTGAAGAAATAAGTGGTAAGAAGCATACAGAAGACACTATTTCTGTGGCGTCATCTTTACATTCTAG TCCTCCTGCATCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTTATTCCATCAGCTAAATCTGACAAC TTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGGTCCAGCATCGTGAGCAATTGTTCTGTTGACTCCA TGTCTGCAGCTCTACAGGATGAACGGTGTTCCTCTCAGGCCCTGGCAGTCCCTGAATCCACTGGGGCATT GGAAAAGACAGAGCACGCTTCAGGGATAGGAGATCATAGTCAACATGGCCCTGGGTGGACACTCTTGAAG CCATCTCTAATCAAGTGTTTAGCTGTCTCATCGTCTGTGAGCAATGAAGAGATTTCTCAAGAGCATATCA TTATAGAAGCAGCTGACAGTGGTCGTGGAAGTTGGACTTCGTGTTCAAGCAGCTCCCATGACAACTTCCA AAGCCTTCCAAACCCAAAAAGCTGGGATTTTTTGAACTCTTACAGACATACCCATTTGGATGACCCCATT GCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCCTGTTCTAAAAGCTGCTCTAGAACTTGTGGGCAGT GTAAAGGAAGCCTAGAGAGAAAGAGTTGGACCTCCTCCAGTTCTCTGTCTGACACGTATGAACCAAACTA TGGGACAGTTAAACAGAGAGTATTGGAGAGCACCCCAGCTGAGTCATCTGAAGGCTTGGACCCCAAGGAT GCCACTGACCCAGTTTATAAAACTGTCACTTCAAGTACAGAAAAGGGCTTGATTGTGTACTGTGTCACCT CACCCAAGAAGGACGATAGGTATAGGGAGCCACCTCCCACTCCTCCAGGATATTTGGGGATTTCTTTAGC GGACCTAAAGGAAGGACCCCACACACACCTAAAACCTCCAGATTATAGTGTGGCAGTGCAGAGGTCAAAG ATGATGCATAACAGCCTCTCTAGACTGCCACCAGCTTCTCTCAGTAGCAACCTCGTGGCCTGTGTTCCAT CGAAGATTGTAACTCAGCCTCAGAGGCATAATTTGCAGCCATTCCATCCTAAACTAGGAGATGTGACTGA TGCAGATAGCGAAGCAGATGAAAATGAACAAGTTTCAGCAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217657 representing NM_016340
Red=Cloning site Green=Tags(s) MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETI ARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRR RFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA TESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPA FANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTL DKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFE GDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEG VEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVP HIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKD TTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC SDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQE TFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHL QDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQV VRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDV ETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLH PIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDN LSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLK PSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPI AEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKD ATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSK MMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016340 |
ORF Size | 4803 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016340.3, NM_016340.4, NM_016340.5, NP_057424.2, NP_057424.3 |
RefSeq Size | 6390 |
RefSeq ORF | 4806 |
Locus ID | 51735 |
Domains | RA, cNMP, RasGEFN, PDZ, RasGEF |
MW | 179.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC308895 | RAPGEF6 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2 |
USD 3,240.00 |
|
RG217657 | RAPGEF6 (GFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2 |
USD 1,850.00 |
|
RC217657L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2, Myc-DDK-tagged |
USD 1,880.00 |
|
RC217657L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 2, mGFP tagged |
USD 1,880.00 |
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