RNPS1 (NM_006711) Human Tagged ORF Clone
CAT#: RC217673
RNPS1 (Myc-DDK-tagged)-Human RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1
"NM_006711" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RNPS1 |
Synonyms | E5.1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217673 representing NM_006711
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTATCAGGAGTGAAAAAGAAGAGCTTGCTAGGAGTCAAAGAAAATAATAAAAAGTCCAGCACTA GGGCTCCTTCACCTACCAAACGCAAAGACCGCTCAGATGAGAAGTCCAAGGATCGCTCAAAAGATAAAGG GGCCACCAAGGAGTCGAGTGAGAAGGATCGCGGCCGGGACAAAACCCGAAAGAGGCGCAGCGCTTCCAGT GGTAGCAGCAGTACCAGGTCTCGGTCCAGCTCGACTTCCAGCTCAGGCTCCAGCACCAGCACTGGCTCAA GCAGTGGCTCCAGCTCTTCCTCAGCATCCAGCCGCTCAGGAAGCTCCAGCACCTCCCGCAGCTCCAGCTC TAGCAGCTCTTCTGGCTCTCCAAGTCCTTCTCGGCGCAGACACGACAACAGGAGGCGCTCCCGCTCCAAA TCCAAACCACCTAAAAGAGATGAAAAGGAGAGGAAAAGGCGGAGCCCATCTCCTAAGCCCACCAAAGTGC ACATTGGGAGACTCACCCGGAATGTGACAAAGGATCACATCATGGAGATATTTTCCACCTATGGGAAAAT TAAAATGATTGACATGCCCGTGGAAAGGATGCATCCCCATCTGTCCAAAGGCTATGCGTACGTAGAGTTT GAGAATCCAGATGAAGCCGAGAAGGCGCTGAAGCACATGGATGGAGGACAAATTGATGGCCAGGAGATCA CTGCCACCGCCGTGCTGGCCCCCTGGCCTAGGCCACCCCCCAGGAGATTCAGCCCTCCCAGGAGAATGTT GCCACCACCGCCTATGTGGCGCAGGTCTCCCCCACGGATGAGGAGAAGGTCCCGCTCCCCGAGGCGCAGG TCCCCCGTGCGCCGGAGATCACGGTCCCCGGGCCGCCGCCGCCACAGGAGCCGCTCCAGCTCCAACTCCT CCCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217673 representing NM_006711
Red=Cloning site Green=Tags(s) MDLSGVKKKSLLGVKENNKKSSTRAPSPTKRKDRSDEKSKDRSKDKGATKESSEKDRGRDKTRKRRSASS GSSSTRSRSSSTSSSGSSTSTGSSSGSSSSSASSRSGSSSTSRSSSSSSSSGSPSPSRRRHDNRRRSRSK SKPPKRDEKERKRRSPSPKPTKVHIGRLTRNVTKDHIMEIFSTYGKIKMIDMPVERMHPHLSKGYAYVEF ENPDEAEKALKHMDGGQIDGQEITATAVLAPWPRPPPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRR SPVRRRSRSPGRRRHRSRSSSNSSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006711 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006711.1, NM_006711.2, NM_006711.3, NM_006711.4, NP_006702.1 |
RefSeq Size | 2038 bp |
RefSeq ORF | 918 bp |
Locus ID | 10921 |
Domains | RRM |
Protein Families | Transcription Factors |
MW | 34 kDa |
Gene Summary | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC115925 | RNPS1 (untagged)-Human RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1 |
USD 310.00 |
|
RG217673 | RNPS1 (GFP-tagged) - Human RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1 |
USD 460.00 |
|
RC217673L3 | Lenti ORF clone of Human RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC217673L4 | Lenti ORF clone of Human RNA binding protein S1, serine-rich domain (RNPS1), transcript variant 1, mGFP tagged |
USD 620.00 |
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