LRMDA (NM_032024) Human Tagged ORF Clone

CAT#: RC217685

C10orf11 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 11 (C10orf11)

Product Images

Western blot validation of overexpression lysate (Cat# LY410357) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217685 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRMDA
• Synonyms: C10orf11; CDA017
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217685 representing NM_032024
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGTATTTGTCACTCAGCGGCAATCATTCTTCAAATAAAAGGTCACTGGAAGGACTGAGCGCAT
  TCAGGAGCCTGGAGGAACTCATCTTGGACAACAATCAGCTGGGGGACGACCTTGTGTTGCCAGGGTTACC
  CAGACTGCATACCTTAACCCTCAACAAGAACCGAATCACTGATTTGGAGAACCTGCTGGATCACTTGGCA
  GAAGTGACACCAGCTCTGGAGTACCTCAGTCTGCTGGGCAACGTGGCCTGTCCCAACGAGCTGGTCAGCT
  TGGAAAAGGATGAGGAAGACTACAAGAGATACAGATGCTTTGTTCTGTACAAGCTGCCCAACTTGAAATT
  TCTGGATGCCCAGAAAGTAACCAGACAAGAACGAGAGGAGGCGTTGGTCAGAGGAGTCTTCATGAAGGTG
  GTGAAGCCCAAGGCTTCTAGTGAGGACGTTGCCAGCTCCCCGGAGCGCCACTACACGCCCTTGCCTTCTG
  CTTCCAGGGAACTCACCAGTCACCAAGGTGTCCTGGGGAAGTGTCGCTACGTTTACTATGGGAAAAACTC
  AGAGGGCAACAGGTTTATCCGAGATGACCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217685 representing NM_032024
  Red=Cloning site Green=Tags(s)
  
  MEKYLSLSGNHSSNKRSLEGLSAFRSLEELILDNNQLGDDLVLPGLPRLHTLTLNKNRITDLENLLDHLA
  EVTPALEYLSLLGNVACPNELVSLEKDEEDYKRYRCFVLYKLPNLKFLDAQKVTRQEREEALVRGVFMKV
  VKPKASSEDVASSPERHYTPLPSASRELTSHQGVLGKCRYVYYGKNSEGNRFIRDDQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032024
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032024.1, NM_032024.2, NM_032024.3, NM_032024.4, NP_114413.1
• RefSeq Size: 902
• RefSeq ORF: 597
• Locus ID: 83938
• MW: 22.4 kDa
• Gene Summary: This gene encodes a leucine-rich repeat protein. The encoded protein is thought to play a role in melanocyte differentiation. Mutations in this gene have been associated with autosomal recessive oculocutaneous albinism 7 (OCA7). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]