SMPDL3B (NM_014474) Human Tagged ORF Clone

CAT#: RC217688

SMPDL3B (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415256) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217688 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMPDL3B
• Synonyms: ASML3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217688 representing NM_014474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGCTCGCCTGGCTGATTTTCCTGGCTAACTGGGGAGGTGCCAGGGCTGAACCAGGGAAGTTCT
  GGCACATCGCTGACCTGCACCTTGACCCTGACTACAAGGTATCCAAAGACCCCTTCCAGGTGTGCCCATC
  AGCTGGATCCCAGCCAGTGCCCGACGCAGGCCCCTGGGGTGACTACCTCTGTGATTCTCCCTGGGCCCTC
  ATCAACTCCTCCATCTATGCCATGAAGGAGATTGAGCCAGAGCCAGACTTCATTCTCTGGACTGGTGATG
  ACACGCCTCATGTGCCCGATGAGAAACTGGGAGAGGCAGCTGTACTGGAAATTGTGGAACGCCTGACCAA
  GCTCATCAGAGAGGTCTTTCCAGATACTAAAGTCTATGCTGCTTTGGGAAATCATGATTTTCACCCCAAA
  AACCAGTTCCCAGCTGGAAGTAACAACATCTACAATCAGATAGCAGAACTATGGAAACCCTGGCTTAGTA
  ATGAGTCCATCGCTCTCTTCAAAAAAGGTGCCTTCTACTGTGAGAAGCTGCCGGGTCCCAGCGGGGCTGG
  GCGAATTGTGGTCCTCAACACCAATCTGTACTATACCAGCAATGCGCTGACAGCAGACATGGCGGACCCT
  GGCCAGCAGTTCCAGTGGCTGGAAGATGTGCTGACCGATGCATCCAAAGCTGGGGACATGGTGTACATTG
  TCGGCCACGTGCCCCCGGGGTTCTTTGAGAAGACGCAAAACAAGGCATGGTTCCGGGAGGGCTTCAATGA
  AAAATACCTGAAGGTGGTCCGGAAGCATCATCGCGTCATAGCAGGGCAGTTCTTCGGGCACCACCACACC
  GACAGCTTTCGGATGCTCTATGATGATGCAGGTGTCCCCATAAGCGCCATGTTCATCACACCTGGAGTCA
  CCCCATGGAAAACCACATTACCTGGAGTGGTCAATGGGGCCAACAATCCAGCCATCCGGGTGTTCGAATA
  TGACCGAGCCACACTGAGCCTGAAGGACATGGTGACCTACTTCATGAACCTGAGCCAGGCGAATGCTCAG
  GGGACGCCGCGCTGGGAGCTCGAGTACCAGCTGACCGAGGCCTATGGGGTGCCGGACGCCAGCGCCCACT
  CCATGCACACAGTGCTGGACCGCATCGCTGGCGACCAGAGCACACTGCAGCGCTACTACGTCTATAACTC
  AGTCAGCTACTCTGCTGGGGTCTGCGACGAGGCCTGCAGCATGCAGCACGTGTGTGCCATGCGCCAGGTG
  GACATTGACGCTTACACCACCTGTCTGTATGCCTCTGGCACCACGCCCGTGCCCCAGCTCCCGCTGCTGC
  TGATGGCCCTGCTGGGCCTGTGCACGCTCGTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217688 representing NM_014474
  Red=Cloning site Green=Tags(s)
  
  MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWAL
  INSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPK
  NQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADP
  GQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHT
  DSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKDMVTYFMNLSQANAQ
  GTPRWELEYQLTEAYGVPDASAHSMHTVLDRIAGDQSTLQRYYVYNSVSYSAGVCDEACSMQHVCAMRQV
  DIDAYTTCLYASGTTPVPQLPLLLMALLGLCTLVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014474
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014474.2, NM_014474.3, NP_055289.2
• RefSeq Size: 1890 bp
• RefSeq ORF: 1368 bp
• Locus ID: 27293
• Domains: Metallophos
• Protein Families: Secreted Protein
• MW: 50.6 kDa