RAP1GDS1 (NM_001100429) Human Tagged ORF Clone

CAT#: RC217939

RAP1GDS1 (Myc-DDK-tagged)-Human RAP1, GTP-GDP dissociation stimulator 1 (RAP1GDS1), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY420268) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217939 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAP1GDS1
• Synonyms: GDS1; SmgGDS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217939 representing NM_001100429
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAATCTCAGTGATACCTTGAAGAAGCTGAAGATAACAGCTGTTGACAAGACTGAGGATAGTTTAG
  AAGGATGCTTGGATTGTCTGCTTCAAGCCCTGGCTCAAAATAATACGGAAACAAGTGAAAAAATCCAAGC
  AAGTGGAATACTTCAGCTGTTTGCAAGTCTGTTGACTCCACAGTCTTCCTGCAAAGCCAAAGTAGCTAAC
  ATCATAGCAGAAGTAGCCAAAAATGAGTTTATGCGAATTCCATGTGTGGATGCTGGATTGATTTCACCAC
  TGGTGCAGCTGCTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCTCTAGGAAACATATG
  TTACGATAGCCATTCGCTTCAAGCTCAGCTTATCAATATGGGTGTTATTCCTACCTTAGTGAAATTACTG
  GGCATCCACTGCCAAAATGCAGCTCTTACAGAAATGTGTCTTGTTGCATTTGGTAATTTAGCAGAACTTG
  AGTCAAGTAAAGAACAGTTTGCCAGTACAAACATTGCTGAAGAGCTAGTAAAACTCTTCAAGAAACAAAT
  AGAACATGATAAGAGAGAAATGATTTTTGAAGTTCTTGCTCCATTGGCAGAAAATGATGCTATTAAACTA
  CAGCTGGTTGAAGCAGGCCTAGTAGAGTGTCTACTAGAGATTGTTCAGCAAAAAGTGGATAGTGACAAAG
  AAGATGATATTACTGAGCTCAAAACTGGTTCAGATCTCATGGTTTTATTACTTCTTGGAGATGAATCCAT
  GCAGAAGTTATTTGAAGGAGGAAAAGGTAGTGTATTTCAAAGGGTACTCTCTTGGATCCCATCAAATAAC
  CACCAGCTACAGCTTGCTGGAGCATTGGCAATTGCAAATTTTGCCAGAAATGATGCAAATTGTATTCATA
  TGGTAGACAATGGGATTGTAGAAAAACTTATGGATTTACTGGACAGACATGTAGAAGATGGAAATGTAAC
  AGTACAGCATGCAGCACTAAGTGCCCTCAGAAACCTGGCCATTCCAGTTATAAATAAAGCAAAGATGTTA
  TCAGCTGGGGTCACAGAGGCAGTTTTGAAATTTCTTAAATCTGAAATGCCTCCTGTTCAGTTCAAACTTC
  TGGGAACATTAAGAATGTTAATAGATGCACAAGCAGAAGCTGCTGAACAATTGGGAAAGAATGTTAAGTT
  AGTGGAGCGTTTGGTGGAATGGTGTGAAGCCAAAGATCATGCTGGTGTGATGGGGGAGTCAAACAGACTG
  CTGTCTGCCCTTATACGACACAGTAAATCAAAAGATGTAATTAAAACCATTGTGCAGAGTGGTGGCATCA
  AGCATCTAGTTACCATGGCAACTAGTGAACATGTAATAATGCAGAATGAAGCTCTTGTTGCTTTGGCATT
  AATAGCAGCTTTAGAATTGGGCACTGCTGAGAAAGATCTAGAAAGTGCTAAACTTGTACAGATTTTACAT
  AGACTGCTAGCAGATGAGAGAAGTGCTCCTGAAATCAAATATAATTCCATGGTCCTGATATGTGCTCTTA
  TGGGATCTGAATGTCTACACAAGGAAGTACAGGATTTGGCTTTTCTAGATGTCGTATCCAAACTTCGCAG
  TCATGAGAACAAAAGTGTTGCCCAGCAGGCCTCTCTCACAGAGCAGAGACTTACTGTGGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217939 representing NM_001100429
  Red=Cloning site Green=Tags(s)
  
  MDNLSDTLKKLKITAVDKTEDSLEGCLDCLLQALAQNNTETSEKIQASGILQLFASLLTPQSSCKAKVAN
  IIAEVAKNEFMRIPCVDAGLISPLVQLLNSKDQEVLLQTGRALGNICYDSHSLQAQLINMGVIPTLVKLL
  GIHCQNAALTEMCLVAFGNLAELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKL
  QLVEAGLVECLLEIVQQKVDSDKEDDITELKTGSDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNN
  HQLQLAGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVINKAKML
  SAGVTEAVLKFLKSEMPPVQFKLLGTLRMLIDAQAEAAEQLGKNVKLVERLVEWCEAKDHAGVMGESNRL
  LSALIRHSKSKDVIKTIVQSGGIKHLVTMATSEHVIMQNEALVALALIAALELGTAEKDLESAKLVQILH
  RLLADERSAPEIKYNSMVLICALMGSECLHKEVQDLAFLDVVSKLRSHENKSVAQQASLTEQRLTVES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100429
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100429.1, NP_001093899.1
• RefSeq Size: 3620 bp
• RefSeq ORF: 1677 bp
• Locus ID: 5910
• Cytogenetics: 4q23
• MW: 60.9 kDa
• Gene Summary: 'The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]'