ANKFN1 (NM_153228) Human Tagged ORF Clone

CAT#: RC217952

ANKFN1 (Myc-DDK-tagged)-Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY407154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217952 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKFN1
• Synonyms: FLJ38335
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217952 representing NM_153228
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGTCTCTAACCAGGAGGCTACTCTTTAAAGACAGGCATTTTACTTGCAGCAAAATAATAGGAA
  GGAGATTCGCTTGCTTTGCACAGAGGCTGAGCCACAGGAGAAAGCAAAGCCAATGTGATTTATTGAATGA
  AAGCACTGGACAATTACCAACAACTTGTTCCTCTGCTGCCTCGAACAGCATAAACTGGAATTGTCGTGTG
  AAAATGACGCAACAAATGCAAAATTTACATCTCTGTCAGTCAAAAAAACATAGTGCTCCCTCATCTCCCA
  ACGCAGCCAAACGCCTGTACAGGAACCTCTCTGAGAAACTGAAAGGGAGCCACTCTTCCTTCGATGAGGC
  CTATTTTAGGACAAGAACTGATCGGCTGAGTCTCAGGAAGACCTCGGTGAATTTCCAGGGCAATGAAGCC
  ATGTTTGAGGCAGTCGAACAGCAGGACATGGATGCTGTGCAGATCCTCCTGTATCAGTACACACCAGAAG
  AACTTGACCTCAACACACCTAACAGCGAGGGCTTGACACCCCTGGATATTGCCATCATGACCAACAATGT
  GCCCATTGCAAGGATTCTTCTGAGGACAGGGGCCCGAGAAAGTCCACACTTTGTCAGCCTGGAAAGCCGA
  GCAATGCACCTCAACACACTGGTCCAGGAAGCCCAGGAGAGGGTGAGTGAACTGTCTGCCCAGGTGGAGA
  ATGAAGGATTCACTCTGGACAACACAGAGAAAGAGAAGCAGCTGAAAGCTTGGGAGTGGAGGTATCGGCT
  CTACAGACGCATGAAAACAGGCTTTGAGCATGCCAGAGCCCCTGAGATGCCAACCAATGTCTGTCTCATG
  GTAACCAGCAGCACATCACTCACTGTCAGCTTCCAAGAGCCTCTTAGCGTCAATGCAGCTGTAGTAACCA
  GGTATAAAGTGGAATGGAGTATGTCCGAAGACTTTTCTCCTTTGGCTGGAGAAATCATCATGGATAATCT
  GCAGACTCTGAGATGCACAATCACAGGACTTACAATGGGCCAACAGTATTTTGTTCAAGTCTCGGCTTAC
  AATATGAAAGGATGGGGACCTGCTCAGACCACGACACCGGCATGTGCCTCTCCTTCTAACTGGAAAGACT
  ATGACGACAGAGAGCCCAGACACAAGGGACAGAGTGAAGTTTTGGAAGGTCTGCTGCAGCAGGTCCGAGC
  CCTTCATCAGCATTACAGTTGCCGGGAAAGCACAAAATTACAAACCACAGGCCGCAAGCAGTCAGTCTCA
  AGAAGCCTGAAACACCTGTTCCATTCCTCGAACAAGTTTGTGAAGACCTTAAAACGGGGACTCTACATAG
  CCGTTATATTTTATTACAAAGACAATATCTTAGTCACCAATGAAGATCAAGTACCAATTGTTGAAATAGA
  TGACTCTCACACCAGTTCTATTACACAAGATTTTCTGTGGTTCACGAAGCTGTCTTGTATGTGGGAAGAT
  ATAAGGTGGCTGAGGCAAAGCATACCAATATCCTCATCCTCATCCACAGTGCTGCAAACTCGGCAGAAGA
  TGCTCGCAGCAACAGCACAGCTACAGAATTTACTTGGGACACACAACTTGGGAAGAGTTTACTATGAGCC
  CATTAAAGATCGACATGGAAACATACTCATAGTCACCATCAGGGAGGTGGAGATGCTTTATTCATTTTTT
  AATGGCAAATGGATGCAGATCTCAAAGCTGCAAAGCCAGAGAAAGTCTCTATCAACACCTGAGGAGCCAA
  CAGCTTTAGACATTCTACTGATAACCATCCAGGATATTCTATCCTATCACAAAAGGAGTCATCAGCGTCT
  CTTTCCTGGATTATATCTGGGTTACCTAAAGCTCTGTAGCTCTGTGGATCAAATCAAAGTTCTTGTTACC
  CAAAAGTTGCCCAACATTCTCTGCCACGTGAAGATCCGTGAAAACAATAATATTTCTAGAGAGGAATGGG
  AATGGATCCAAAAGCTTTCTGGCTCTGAATCTATGGAAAGTGTGGATCATACTTCTGACTGCCCCATGCA
  ATTGTTCTTCTACGAGCTCCAGATGGCAGTGAAAGCTCTCCTTCAGCAGATCAATATACCTCTACACCAG
  GCAAGGAACTTCCGCCTCTACACACAGGAGGTGTTGGAAATGGGTCACAATGTGTCCTTTCTTCTCCTGC
  TCCCTGCCTCAGACGACGTCTGTACAGCCCCAGGACAGAATAATCCTTACACCCCACACTCAGGGTTTCT
  TAACCTCCCTCTTCAGATGTTTGAACTTGGTATAGTAGCTTGTTTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217952 representing NM_153228
  Red=Cloning site Green=Tags(s)
  
  MEASLTRRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRV
  KMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEA
  MFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESR
  AMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLM
  VTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAY
  NMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVS
  RSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWED
  IRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFF
  NGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVT
  QKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQ
  ARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELGIVACFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153228
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153228.1, NM_153228.2, NP_694960.1, NP_694960.2
• RefSeq Size: 2426
• RefSeq ORF: 2292
• Locus ID: 162282
• Domains: ANK, FN3
• Protein Families: Druggable Genome
• MW: 87.4 kDa