SPON1 (NM_006108) Human Tagged ORF Clone

CAT#: RC218131

SPON1 (Myc-DDK-tagged)-Human spondin 1, extracellular matrix protein (SPON1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401846) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218131 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPON1
• Synonyms: f-spondin; VSGP/F-spondin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218131 representing NM_006108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGTCCCCGGCGCCCCTGAAGCTGAGCCGGACTCCGGCACTGCTGGCCCTGGCGCTGCCCCTGG
  CCGCGGCGCTGGCCTTCTCCGACGAGACCCTGGACAAAGTGCCCAAGTCAGAGGGCTACTGCAGCCGTAT
  CCTGCGCGCCCAGGGCACGCGGCGCGAGGGCTACACCGAGTTCAGCCTCCGCGTGGAGGGCGACCCCGAC
  TTCTACAAGCCGGGAACCAGCTACCGCGTAACACTTTCAGCTGCTCCTCCCTCCTACTTCAGAGGATTCA
  CATTAATTGCCCTCAGAGAGAACAGAGAGGGTGATAAGGAAGAAGACCATGCTGGGACCTTCCAGATCAT
  AGACGAAGAAGAAACTCAGTTTATGAGCAATTGCCCTGTTGCAGTCACTGAAAGCACTCCACGGAGGAGG
  ACCCGGATCCAGGTGTTTTGGATAGCACCACCAGCGGGAACAGGCTGCGTGATTCTGAAGGCCAGCATCG
  TACAAAAACGCATTATTTATTTTCAAGATGAGGGCTCTCTGACCAAGAAACTTTGTGAACAAGATTCCAC
  ATTTGATGGGGTGACTGACAAACCCATCTTAGACTGCTGTGCCTGCGGAACTGCCAAGTACAGACTCACA
  TTTTATGGGAATTGGTCCGAGAAGACACACCCAAAGGATTACCCTCGTCGGGCCAACCACTGGTCTGCGA
  TCATCGGAGGATCCCACTCCAAGAATTATGTACTGTGGGAATATGGAGGATATGCCAGCGAAGGCGTCAA
  ACAAGTTGCAGAATTGGGCTCACCCGTGAAAATGGAGGAAGAAATTCGACAACAGAGTGATGAGGTCCTC
  ACCGTCATCAAAGCCAAAGCCCAGTGGCCAGCCTGGCAGCCTCTCAACGTGAGAGCAGCACCTTCAGCTG
  AATTTTCCGTGGACAGAACGCGCCATTTAATGTCCTTCCTGACCATGATGGGCCCTAGTCCCGACTGGAA
  CGTAGGCTTATCTGCAGAAGATCTGTGCACCAAGGAATGTGGCTGGGTCCAGAAGGTGGTGCAAGACCTG
  ATTCCCTGGGACGCTGGCACCGACAGCGGGGTGACCTATGAGTCACCCAACAAACCCACCATTCCCCAGG
  AGAAAATCCGGCCCCTGACCAGCCTGGACCATCCTCAGAGTCCTTTCTATGACCCAGAGGGTGGGTCCAT
  CACTCAAGTAGCCAGAGTTGTCATCGAGAGAATCGCACGGAAGGGTGAACAATGCAATATTGTACCTGAC
  AATGTCGATGATATTGTAGCTGACCTGGCTCCAGAAGAGAAAGATGAAGATGACACCCCTGAAACCTGCA
  TCTACTCCAACTGGTCCCCATGGTCCGCCTGCAGCTCCTCCACCTGTGACAAAGGCAAGAGGATGCGACA
  GCGCATGCTGAAAGCACAGCTGGACCTCAGCGTCCCCTGCCCTGACACCCAGGACTTCCAGCCCTGCATG
  GGCCCTGGCTGCAGTGACGAAGACGGCCCCACCTGCACCATGTCCGAGTGGATCACCTGGTCGCCCTGCA
  GCATCTCCTGCGGCATGGGCATGAGGTCCCGGGAGAGGTATGTGAAGCAGTTCCCGGAGGACGGCTCCGT
  GTGCACGCTGCCCACTGAGGAAACGGAGAAGTGCACGGTCAACGAGGAGTGCTCTCCCAGCAGCTGCCTG
  ATGACCGAGTGGGGCGAGTGGGACGAGTACAGCGCCACCTGCGGCATGGGCATGAAGAAGCGGCACCGCA
  TGATCAAGATGAACCCCGCAGATGGCTCCATGTGCAAAGCCGAGACATCACAGGCAGAGAAGCGCATGAT
  GCCAGAGTGCCACACCATCCCATGCTTGCTGTCCCCATGGTCCGAGTGGAGTGACTGCAGCGTGACCTGC
  GGGAAGGGCATGCGAACCCGACAGCGGATGCTCAAGTCTCTGGCAGAACTTGGAGACTGCAATGAGGATC
  TGGAGCAGGTGGAGAAGTGCATGCTCCCTGAATGCCCCATTGACTGTGAGCTCACCGAGTGGTCCCAGTG
  GTCGGAATGTAACAAGTCATGTGGGAAAGGCCACGTGATTCGAACCCGGATGATCCAAATGGAGCCTCAG
  TTTGGAGGTGCACCCTGCCCAGAGACTGTGCAGCGAAAAAAGTGCCGCATCCGAAAATGCCTTCGAAATC
  CATCCATCCAAAAGCTACGCTGGAGGGAGGCCCGAGAGAGCCGGCGGAGTGAGCAGCTGAAGGAAGAGTC
  TGAAGGGGAGCAGTTCCCAGGTTGTAGGATGCGCCCATGGACGGCCTGGTCAGAATGCACCAAACTGTGC
  GGAGGTGGAATTCAGGAACGTTACATGACTGTAAAGAAGAGATTCAAAAGCTCCCAGTTTACCAGCTGCA
  AAGACAAGAAGGAGATCAGAGCATGCAATGTTCATCCTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218131 representing NM_006108
  Red=Cloning site Green=Tags(s)
  
  MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYCSRILRAQGTRREGYTEFSLRVEGDPD
  FYKPGTSYRVTLSAAPPSYFRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRR
  TRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLT
  FYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVL
  TVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDL
  IPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPD
  NVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM
  GPGCSDEDGPTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCL
  MTEWGEWDEYSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKRMMPECHTIPCLLSPWSEWSDCSVTC
  GKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNKSCGKGHVIRTRMIQMEPQ
  FGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRSEQLKEESEGEQFPGCRMRPWTAWSECTKLC
  GGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006108
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006108.1, NM_006108.2, NM_006108.3, NP_006099.1
• RefSeq Size: 4755 bp
• RefSeq ORF: 2424 bp
• Locus ID: 10418
• Domains: tsp_1, Reeler
• Protein Families: Secreted Protein
• MW: 90.8 kDa