ZNF215 (NM_013250) Human Tagged ORF Clone

CAT#: RC218195

ZNF215 (Myc-DDK-tagged)-Human zinc finger protein 215 (ZNF215)

Product Images

Western blot validation of overexpression lysate (Cat# LY415709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218195 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF215
• Synonyms: BAZ-2; BAZ2; ZKSCAN11; ZSCAN43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218195 representing NM_013250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCTCTGAGCAAGTTGATGGCTATCTCAAAACCTCGAAACCTGTCTCTACGTGAACAAAGAGAGG
  TTCTGAGAGCAGATATGTCTTGGCAGCAGGAAACCAACCCCGTCGTGGAGACACATGACTCTGAGGCATC
  TCGTCAAAAGTTCAGACATTTCCAGTATTTGAAAGTGTCTGGGCCCCATGAAGCCCTGAGCCAACTCTGG
  GAGCTCTGTCTTCAATGGCTGAGACCAGAGATTCATACAAAGAAGCAGATTATAGAACTGTTGGTGCTGG
  AACAATTCCTGGCAATCCTGCCTGAAGAAGTCAGGACTTGGGTGAATTTACAACATCCAAACAACAGTAA
  AGATATGGTGACCCTCATAGAAGATGTGATTGAAATGCTTGAAGATGAAGATATGCCCTGCAAGGACTCT
  GCCCTGCAGATGGGGAGCATCAAGGAGAAAATGAAAGCTGGCTCACGAACAGGCAAACCACAGGAACCAG
  TGACATTCAAAGATGTGGTTGTGGAATTCAGCAAGGAAGAGTGGGGGCAACTGGACTCTGCTGTAAAGAA
  CCTGTACAGGAATGTGATGCTGGAAAACTTTAGGAACCTGAATTCATTGCGTAAAGCACATCTACTTTCC
  AAACCATTTGAGAGCCTTAAGTTGGAGAGTAAGAAAAAAAGATGGATAATGGAGAAAGAAATACCAAGGA
  AGACTATTTTTGACATGAAGAGTATTTCTGGAGAAGAATCATCCCATGGAGTGATTATGACAAGGCTTAC
  CGAAAGTGGACACCCTTCTTCAGATGCCTGGAAAGGTGAGAATTGGTTATATAGGAACCAGAAAAAATGG
  GACATAAATTTGCCACAAGAGGCTTTCATTCCTGAGACAATTTACACTGAGGAGGAAGATTTTGAATGTA
  GTGAAAATAAGAAAAGCTTTGATATTAATTCAGTTAGCTCAATTTGTGCTATACAAGTGGGAATTCCTTC
  AAGAAAGGGGTCTCCAAAATGTGATAAGTTTAAAACTTACTTCAAATTTAATTTAGACTCAGTAGGTAAG
  CAACATTCAGAATATGAATATGGGAATGACTTGAGTTTGAGTACAGATATTCGACACCAAAAAAGTCATA
  CTACAATGAATTCCTATGAATGTTATCAATGTGGGAAAGCCTTCTGCCGAAGTTCATCCCTTATTCGACA
  TCAGATCATTCACACAGGAGAGAAACCCTATAAATGCAGTGAATGTGGGAGATTCTTCAACCGACGTACA
  AACCTTACTAAGCATCAAAAACTTCATGCTGAAGCAAAGGCCTGCACAAGCAATAAATGTGGAAAGGCCT
  TCAGTAAAAGTGAAGACAGTAATAATCCAACACTCCATTTTGGAAACAATTTCTATCAATGTGTTAACTG
  TGGAAAATCCTTCAACCGGAGCTCCTCTCTTATTCGACACCAAATGATTCACACGGGAGAGAAACCATTC
  AAATGTAAGGAATGTAGTAAAGCCTTCAACAGGAGTTCAAACCTTGTTAAACATCAAAAACTGCATACTC
  GAGATAAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218195 representing NM_013250
  Red=Cloning site Green=Tags(s)
  
  MQPLSKLMAISKPRNLSLREQREVLRADMSWQQETNPVVETHDSEASRQKFRHFQYLKVSGPHEALSQLW
  ELCLQWLRPEIHTKKQIIELLVLEQFLAILPEEVRTWVNLQHPNNSKDMVTLIEDVIEMLEDEDMPCKDS
  ALQMGSIKEKMKAGSRTGKPQEPVTFKDVVVEFSKEEWGQLDSAVKNLYRNVMLENFRNLNSLRKAHLLS
  KPFESLKLESKKKRWIMEKEIPRKTIFDMKSISGEESSHGVIMTRLTESGHPSSDAWKGENWLYRNQKKW
  DINLPQEAFIPETIYTEEEDFECSENKKSFDINSVSSICAIQVGIPSRKGSPKCDKFKTYFKFNLDSVGK
  QHSEYEYGNDLSLSTDIRHQKSHTTMNSYECYQCGKAFCRSSSLIRHQIIHTGEKPYKCSECGRFFNRRT
  NLTKHQKLHAEAKACTSNKCGKAFSKSEDSNNPTLHFGNNFYQCVNCGKSFNRSSSLIRHQMIHTGEKPF
  KCKECSKAFNRSSNLVKHQKLHTRDKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013250
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013250.1, NM_013250.2, NP_037382.1, NP_037382.2
• RefSeq Size: 3480
• RefSeq ORF: 1554
• Locus ID: 7762
• Protein Families: Transcription Factors
• MW: 59.9 kDa
• Gene Summary: This gene is imprinted in a tissue-specific manner with preferential expression in the testis, and encodes a zinc finger protein that belongs to a family of zinc finger transcription factors. The encoded protein contains an N-terminal SRE-ZBP, Ctfin51, AW-1, and Number 18 (SCAN) domain, a kruppel-associated box A (KRABA) domain, and four C-terminal zinc finger domains. This gene is located within one of three regions on chromosome 11p15 associated with Beckwith-Wiedemann syndrome, called Beckwith-Wiedemann syndrome chromosome region-2 (BWSCR2), and is thought to play a role in the etiology of this disease. [provided by RefSeq, Aug 2017]