SF3A1 (NM_001005409) Human Tagged ORF Clone

CAT#: RC218372

SF3A1 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SF3A1
• Synonyms: PRP21; PRPF21; SAP114; SF3A120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218372 representing NM_001005409
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCGGACCCGTGCAGGCGGTGCCCCCGCCGCCGCCCGTGCCCACGGAGCCCAAACAGCCCACAG
  AAGAAGAAGCATCTTCAAAGGAGGATTCTGCACCTTCTAAGCCAGTTGTGGGGATTATTTACCCTCCTCC
  AGAGGTCAGAAATATTGTTGACAAGACTGCCAGCTTTGTGGCCAGAAACGGGCCTGAATTTGAAGCTAGG
  ATCCGACAGAACGAGATCAACAACCCCAAGTTCAACTTTCTGAACCCCAATGACCCTTACCATGCCTACT
  ACCGCCACAAGGTCAGCGAGTTCAAGGAAGGGAAGGCTCAGTTTGTGGCCAGGAATGGGCGCCAGTTTCT
  GACCCAGCTGATGCAGAAAGAGCAGCGCAACTACCAGTTTGACTTTCTCCGCCCACAGCACAGCCTCTTC
  AACTACTTCACGAAGCTAGTGGAACAGTACACCAAGATCTTGATTCCACCCAAAGGTTTATTTTCAAAGC
  TCAAGAAAGAGGCTGAAAACCCCCGAGAAGTTTTGGATCAGGTGTGTTACCGAGTGGAATGGGCCAAATT
  CCAGGAACGTGAGAGGAAGAAGGAAGAAGAGGAGAAGGAGAAGGAGCGGGTGGCCTATGCTCAGATCGAC
  TGGCATGATTTTGTGGTGGTGGAAACAGTGGACTTCCAACCCAATGAGCAAGGGAACTTCCCTCCCCCCA
  CCACGCCAGAGGAGCTGGGGGCCCGAATCCTCATTCAGGAGCGCTATGAAAAGTTTGGGGAGAGTGAGGA
  AGTTGAGATGGAGGTCGAGTCTGATGAGGAGGATGACAAACAGGAGAAGGCGGAGGAGCCTCCTTCCCAG
  CTGGACCAGGACACCCAAGTACAAGATATGGATGAGGGTTCAGATGATGAAGAAGAAGGGCAGAAAGTGC
  CCCCACCCCCAGAGACACCCATGCCTCCACCTCTGCCCCCAACTCCAGACCAAGTCATTGTCCGCAAGGA
  TTATGATCCCAAAGCCTCCAAGCCCTTGCCTCCAGCCCCTGCTCCAGATGAGTATCTTGTGTCCCCCATT
  ACTGGGGAGAAGATCCCCGCCAGCAAAATGCAGGAACACATGCGCATTGGACTTCTTGACCCTCGCTGGC
  TGGAGCAGCGGGATCGCTCCATCCGTGAGAAGCAGAGCGATGATGAGGTGTACGCACCAGGTCTGGATAT
  TGAGAGCAGCTTGAAGCAGTTGGCTGAGCGGCGTACTGACATCTTCGGTGTAGAGGAAACAGCCATTGGT
  AAGAAGATCGGTGAGGAGGAGATCCAGAAGCCAGAGGAAAAGGTGACCTGGGATGGCCACTCAGGCAGCA
  TGGCCCGGACCCAGCAGGCTGCCCAGGCCAACATCACCCTCCAGGAGCAGATTGAGGCCATTCACAAGGC
  CAAAGGCCTGGTGCCAGAGGATGACACTAAAGAGAAGATTGGCCCCAGCAAGCCCAATGAAATCCCTCAA
  CAGCCACCGCCACCATCTTCAGCCACCAACATCCCCAGCTCGGCTCCACCCATCACTTCAGTGCCCCGAC
  CACCCACAATGCCACCTCCAGTTCGTACTACAGTTGTCTCCGCAGTACCCGTCATGCCCCGGCCCCCAAT
  GGCATCTGTGGTCCGGCTGCCCCCAGGCTCAGTGATCGCCCCCATGCCGCCCATCATCCACGCGCCCAGA
  ATCAACGTGGTGCCCATGCCTCCCTCGGCCCCTCCTATTATGGCCCCCCGCCCACCCCCCATGATTGTGC
  CAACAGCCTTTGTGCCTGCTCCACCTGTGGCACCTGTCCCAGCTCCAGCCCCAATGCCCCCTGTGCATCC
  CCCACCTCCCATGGAAGATGAGCCCACCTCCAAAAAACTGAAGACAGAGGACAGCCTCATGCCAGAGGAG
  GAGTTCCTGCGCAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATAAGACGG
  AATGGAAACTGAATGGGCAGGTGCTGGTCTTCACCCTCCCACTCACGGACCAGGTCTCTGTCATTAAGGT
  GAAGATTCATGAAGCCACAGGCATGCCTGCAGGGAAACAGAAGCTACAGTATGAGGGTATCTTCATCAAA
  GATTCCAACTCACTGGCTTACTACAACATGGCCAATGGCGCAGTCATCCACCTGGCCCTCAAGGAGAGAG
  GCGGGAGGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218372 representing NM_001005409
  Red=Cloning site Green=Tags(s)
  
  MPAGPVQAVPPPPPVPTEPKQPTEEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEAR
  IRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLF
  NYFTKLVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQID
  WHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDDKQEKAEEPPSQ
  LDQDTQVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPI
  TGEKIPASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIG
  KKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQ
  QPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPR
  INVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEE
  EFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIK
  DSNSLAYYNMANGAVIHLALKERGGRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005409
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001005409.1, NP_001005409.1
• RefSeq Size: 4941
• RefSeq ORF: 2187
• Locus ID: 10291
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• MW: 81.5 kDa
• Gene Summary: This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014]