5HT4 Receptor (HTR4) (NM_199453) Human Tagged ORF Clone

CAT#: RC218409

HTR4 (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant g

Product Images

Western blot validation of overexpression lysate (Cat# LY404572) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218409 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HTR4
• Synonyms: 5-HT4; 5-HT4R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218409 representing NM_199453
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCA
  CGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGGGA
  CAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCG
  GTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTC
  TTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGCTGCATTTCTCTGGA
  TAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTA
  ATGCTGGGAGGCTGCTGGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACA
  TTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCAT
  GGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTG
  GCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAG
  CCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCATAGCACTCATCGCATGAGGACAGAGACCAAAGC
  AGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATT
  GTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCA
  ATTCCGGGTTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCT
  CTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGTTCAACCACAACC
  ATTAATGGATCCACACATGTACTAAGTGGCTGTTCCCCTGTCTCCAGCTTCCTCCTGCTCTTCTGCAATA
  GACCAGTTCCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218409 representing NM_199453
  Red=Cloning site Green=Tags(s)
  
  MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVS
  VLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIAL
  MLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVL
  AYYRIYVTAKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNI
  VDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTT
  INGSTHVLSGCSPVSSFLLLFCNRPVPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199453
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199453.1, NM_199453.2, NM_199453.3, NP_955525.1
• RefSeq Size: 1557 bp
• RefSeq ORF: 1137 bp
• Locus ID: 3360
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 42.6 kDa
• Gene Summary: 'This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]'