IRAK2 (NM_001570) Human Tagged ORF Clone

CAT#: RC218437

IRAK2 (Myc-DDK-tagged)-Human interleukin-1 receptor-associated kinase 2 (IRAK2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRAK2
• Synonyms: IRAK-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218437 representing NM_001570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATGGACGCGCTCA
  GCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACCCAGCTGCGGAAGATCAAGTC
  CATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTGCTGTGGTGGTGGGGCATGCGGCAGGCCACC
  GTCCAGCAACTTGTGGACCTCCTGTGCCGCCTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGA
  AACCGGCTCCTGAAATCAGGTGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGC
  AGCTTCTGTAAGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
  CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGATGCCCCTCATT
  CCTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACCTCCATTCCTAAGCAGGAAAA
  ACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAGGCAGACGTGGTCCAGGCAACCGATGACTTC
  AATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCAT
  TCGTCTTCAAGAAGCTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGA
  GTTGCAGATTTGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
  TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTCAGGGTGGCT
  CGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCA
  TGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAA
  CTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACC
  TGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGA
  CATCTTCAGCTGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
  CCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCGCTCTGCTCCAGGAAGA
  CGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCC
  GGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAG
  GAGGTGTGTGGCTCTGTGGCTGCTGTGGAAGAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTG
  GGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCT
  TGATGCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
  GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGG
  AGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGGCCAAAAGGAAACTGATGGA
  GAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218437 representing NM_001570
  Red=Cloning site Green=Tags(s)
  
  MACYIYQLPSWVLDDLCRNMDALSEWDWMEFASYVITDLTQLRKIKSMERVQGVSITRELLWWWGMRQAT
  VQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPG
  PGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDF
  NQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQ
  FHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSSNVLLDQNLTPK
  LAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRS
  PVYLKDLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQ
  EVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDDVDNSSLDASSSMSVAPWAGAATPLLPTEN
  GEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001570
• ORF Size: 1875 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001570.3, NP_001561.3
• RefSeq Size: 3469 bp
• RefSeq ORF: 1878 bp
• Locus ID: 3656
• Cytogenetics: 3p25.3
• Domains: DEATH, pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway
• MW: 69.3 kDa
• Gene Summary: 'IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq, Jul 2008]'