C14orf151 (INF2) (NM_032714) Human Tagged ORF Clone

CAT#: RC218469

INF2 (Myc-DDK-tagged)-Human inverted formin, FH2 and WH2 domain containing (INF2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY409977) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218469 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified INF2 protein (Cat# TP318469). The protein was produced from HEK293T cells transfected with INF2 cDNA clone (Cat# RC218469) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INF2
• Synonyms: C14orf151; C14orf173; CMTDIE; FSGS5; pp9484
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218469 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGAAGGAGGGCGCACAGCGCAAGTGGGCAGCGCTGAAGGAGAAGCTGGGGCCACAGGATTCGG
  ACCCCACGGAGGCCAACCTGGAGAGCGCGGACCCTGAGCTGTGCATCCGGCTGCTCCAGATGCCCTCTGT
  GGTCAACTACTCCGGCCTGCGCAAGCGCCTGGAGGGCAGCGACGGCGGCTGGATGGTGCAGTTCCTGGAG
  CAGAGCGGCCTGGACCTGCTGCTGGAGGCGCTGGCGCGGCTGTCGGGCCGCGGCGTTGCACGTATCTCCG
  ACGCCCTGCTGCAGCTCACCTGCGTCAGCTGCGTGCGCGCCGTCATGAACTCGCGGCAGGGCATCGAGTA
  CATCCTCAGCAACCAGGGCTACGTGCGCCAGCTCTCCCAGGCCCTGGACACATCCAACGTGATGGTGAAG
  AAGCAGGTGTTTGAGCTACTGGCTGCCCTGTGCATCTACTCTCCCGAGGGCCACGTGCTGACCCTGGACG
  CCCTGGACCACTACAAGACGGTGTGCAGCCAGCAGTACCGCTTCAGCATTGTCATGAACGAGCTCTCCGG
  CAGCGACAACGTGCCCTACGTGGTCACCCTGCTTAGCGTGATCAACGCCGTCATCTTGGGCCCCGAGGAC
  CTGCGCGCGCGCACCCAGCTGCGGAACGAGTTTATCGGGCTGCAGCTGCTGGACGTCCTGGCTCGCCTGC
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218469 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGSDGGWMVQFLE
  QSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVK
  KQVFELLAALCIYSPEGHVLTLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPED
  LRARTQLRNEFIGLQLLDVLARLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032714
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032714.1, NM_032714.2, NP_116103.1
• RefSeq Size: 1719 bp
• RefSeq ORF: 705 bp
• Locus ID: 64423
• Protein Families: Druggable Genome
• MW: 26 kDa
• Gene Summary: This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5. [provided by RefSeq, Aug 2010]