OSGIN1 (NM_013370) Human Tagged ORF Clone

CAT#: RC218475

OSGIN1 (Myc-DDK-tagged)-Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415630) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218475 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OSGIN1 protein (Cat# TP318475). The protein was produced from HEK293T cells transfected with OSGIN1 cDNA clone (Cat# RC218475) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSGIN1
• Synonyms: BDGI; OKL38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218475 representing NM_013370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGTGGAGACCGAGAGGCTGCTGCCGGGGAAATATGCAGTGCAGACAAGAGGTTCCTGCTACTC
  TCACCTCATCAGAGTTGTTTTCTACCAGGAACCAGCCCCAGCCCCAGCCCCAGCCCCTGCTGGCAGATGC
  TCCGGTACCCTGGGCTGTGGCTTCCAGGATGTGCCTGACTCCAGGGCAGGGCTGTGGCCACCAGGGCCAG
  GACGAGGGTCCGCTGCCAGCCCCAAGCCCCCCACCAGCCATGAGCTCCTCCAGAAAGGACCACCTCGGCG
  CCAGCAGCTCAGAGCCCCTCCCGGTCATCATTGTGGGTAACGGCCCCTCTGGTATCTGCCTGTCCTACCT
  GCTCTCCGGCTACACACCCTACACGAAGCCAGATGCCATCCACCCACACCCCCTGCTGCAGAGGAAGCTC
  ACCGAGGCCCCGGGGGTCTCCATCCTGGACCAGGACCTGGACTACCTGTCCGAAGGCCTCGAAGGCCGAT
  CCCAAAGCCCCGTGGCCCTGCTCTTTGATGCCCTTCTACGCCCAGACACAGACTTTGGGGGAAACATGAA
  GTCGGTCCTCACCTGGAAGCACCGGAAGGAGCACGCCATCCCCCACGTGGTTCTGGGCCGGAACCTCCCC
  GGGGGAGCCTGGCACTCCATCGAAGGCTCCATGGTGATCCTGAGCCAAGGCCAGTGGATGGGGCTCCCGG
  ACCTGGAGGTCAAGGACTGGATGCAGAAGAAGCGAAGAGGTCTTCGCAACAGCCGGGCCACTGCCGGGGA
  CATCGCCCACTACTACAGGGACTACGTGGTCAAGAAGGGTCTGGGGCATAACTTTGTGTCCGGTGCTGTA
  GTCACAGCCGTGGAGTGGGGGACCCCCGATCCCAGCAGCTGTGGGGCCCAGGACTCCAGCCCCCTCTTCC
  AGGTGAGCGGCTTCCTGACCAGGAACCAGGCCCAGCAGCCCTTCTCGCTGTGGGCCCGCAACGTGGTCCT
  CGCCACAGGCACGTTCGACAGCCCGGCCCGGCTGGGCATCCCCGGGGAGGCCCTGCCCTTCATCCACCAT
  GAGCTGTCTGCCCTGGAGGCCGCCACAAGGGTGGGTGCGGTGACCCCGGCCTCAGACCCTGTCCTCATCA
  TTGGCGCGGGGCTGTCAGCGGCCGACGCGGTCCTCTACGCCCGCCACTACAACATCCCGGTGATCCATGC
  CTTCCGCCGGGCCGTGGACGACCCTGGCCTGGTGTTCAACCAGCTGCCCAAGATGCTGTACCCCGAGTAC
  CACAAGGTGCACCAGATGATGCGGGAGCAGTCCATCCTGTCGCCCAGCCCCTATGAGGGTTACCGCAGCC
  TCCCCAGGCACCAGCTGCTGTGCTTCAAGGAAGACTGCCAGGCCGTGTTCCAGGACCTCGAGGGTGTCGA
  GAAGGTGTTTGGGGTCTCCCTGGTGCTGGTCCTCATCGGCTCCCACCCCGACCTCTCCTTCCTGCCTGGG
  GCAGGGGCTGACTTTGCAGTGGATCCTGACCAGCCGCTGAGCGCCAAGAGGAACCCCATTGACGTGGACC
  CCTTCACCTACCAGAGCACCCGCCAGGAGGGCCTGTACGCCATGGGGCCGCTGGCCGGGGACAACTTCGT
  GAGGTTTGTGCAGGGGGGCGCCTTGGCTGTGGCCAGCTCCCTGCTAAGGAAGGAGACCAGGAAGCCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218475 representing NM_013370
  Red=Cloning site Green=Tags(s)
  
  MGKWRPRGCCRGNMQCRQEVPATLTSSELFSTRNQPQPQPQPLLADAPVPWAVASRMCLTPGQGCGHQGQ
  DEGPLPAPSPPPAMSSSRKDHLGASSSEPLPVIIVGNGPSGICLSYLLSGYTPYTKPDAIHPHPLLQRKL
  TEAPGVSILDQDLDYLSEGLEGRSQSPVALLFDALLRPDTDFGGNMKSVLTWKHRKEHAIPHVVLGRNLP
  GGAWHSIEGSMVILSQGQWMGLPDLEVKDWMQKKRRGLRNSRATAGDIAHYYRDYVVKKGLGHNFVSGAV
  VTAVEWGTPDPSSCGAQDSSPLFQVSGFLTRNQAQQPFSLWARNVVLATGTFDSPARLGIPGEALPFIHH
  ELSALEAATRVGAVTPASDPVLIIGAGLSAADAVLYARHYNIPVIHAFRRAVDDPGLVFNQLPKMLYPEY
  HKVHQMMREQSILSPSPYEGYRSLPRHQLLCFKEDCQAVFQDLEGVEKVFGVSLVLVLIGSHPDLSFLPG
  AGADFAVDPDQPLSAKRNPIDVDPFTYQSTRQEGLYAMGPLAGDNFVRFVQGGALAVASSLLRKETRKPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013370
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013370.2, NM_013370.3, NP_037502.2, NP_037502.3
• RefSeq Size: 2400
• RefSeq ORF: 1682
• Locus ID: 29948
• MW: 60.7 kDa
• Gene Summary: This gene encodes an oxidative stress response protein that regulates cell death. Expression of the gene is regulated by p53 and is induced by DNA damage. The protein regulates apoptosis by inducing cytochrome c release from mitochondria. It also appears to be a key regulator of both inflammatory and anti-inflammatory molecules. The loss of this protein correlates with uncontrolled cell growth and tumor formation. Naturally occurring read-through transcription exists between this gene and the neighboring upstream malonyl-CoA decarboxylase (MLYCD) gene, but the read-through transcripts are unlikely to produce a protein product. [provided by RefSeq, Aug 2011]