CD1C (NM_001765) Human Tagged ORF Clone

CAT#: RC218490

CD1C (Myc-DDK-tagged)-Human CD1c molecule (CD1C)

Product Images

Western blot validation of overexpression lysate (Cat# LY419762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218490 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CD1C protein (Cat# TP318490). The protein was produced from HEK293T cells transfected with CD1C cDNA clone (Cat# RC218490) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD1C
• Synonyms: BDCA1; CD1; CD1A; R7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218490 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTTCTGCAGTTTCTGCTGCTAGCTCTTCTTCTCCCAGGTGGTGACAATGCAGACGCATCCCAGG
  AACACGTCTCCTTCCATGTCATCCAGATCTTCTCATTTGTCAACCAATCCTGGGCACGAGGTCAGGGCTC
  AGGATGGCTGGACGAGTTGCAGACTCATGGCTGGGACAGTGAATCAGGCACAATAATTTTCCTGCATAAC
  TGGTCCAAGGGCAACTTCAGCAATGAAGAGTTGTCAGACCTAGAGTTGTTATTTCGTTTCTACCTCTTTG
  GATTAACTCGGGAGATTCAAGACCATGCAAGTCAAGATTACTCGAAATATCCCTTTGAAGTACAGGTGAA
  AGCGGGCTGTGAGCTGCATTCTGGAAAGAGCCCAGAAGGCTTCTTTCAGGTAGCTTTCAACGGATTAGAT
  TTACTGAGTTTCCAGAATACAACATGGGTGCCATCTCCAGGCTGTGGAAGTTTGGCCCAAAGTGTCTGTC
  ATCTACTCAATCATCAGTATGAAGGCGTCACAGAAACAGTGTATAATCTCATAAGAAGCACTTGCCCCCG
  ATTTCTCTTGGGTCTCCTGGATGCAGGGAAGATGTATGTACACAGGCAAGTGAGGCCAGAAGCCTGGCTG
  TCCAGTCGCCCCAGCCTTGGGTCTGGCCAGCTGTTGCTGGTTTGTCATGCCTCCGGCTTCTACCCAAAGC
  CTGTTTGGGTGACATGGATGCGGAATGAACAGGAGCAACTGGGCACTAAACATGGTGATATTCTTCCTAA
  TGCTGATGGGACATGGTATCTTCAGGTGATCCTGGAGGTGGCATCTGAGGAGCCTGCTGGCCTGTCTTGT
  CGAGTGAGACACAGCAGTCTAGGAGGCCAGGACATCATCCTCTACTGGGGACACCACTTTTCCATGAATT
  GGATTGCCTTGGTAGTGATAGTGCCCTTGGTGATTCTAATAGTCCTTGTGTTATGGTTTAAGAAGCACTG
  CTCATATCAGGACATCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218490 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLFLQFLLLALLLPGGDNADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHN
  WSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKAGCELHSGKSPEGFFQVAFNGLD
  LLSFQNTTWVPSPGCGSLAQSVCHLLNHQYEGVTETVYNLIRSTCPRFLLGLLDAGKMYVHRQVRPEAWL
  SSRPSLGSGQLLLVCHASGFYPKPVWVTWMRNEQEQLGTKHGDILPNADGTWYLQVILEVASEEPAGLSC
  RVRHSSLGGQDIILYWGHHFSMNWIALVVIVPLVILIVLVLWFKKHCSYQDIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001765
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001765.1, NM_001765.2, NP_001756.1
• RefSeq Size: 2592 bp
• RefSeq ORF: 1002 bp
• Locus ID: 911
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage
• MW: 37.7 kDa
• Gene Summary: This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul 2008]