SALL2 (NM_005407) Human Tagged ORF Clone

CAT#: RC218496

SALL2 (Myc-DDK-tagged)-Human sal-like 2 (Drosophila) (SALL2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SALL2
• Synonyms: COLB; HSAL2; p150(Sal2); Sal-2; ZNF795
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218496 representing NM_005407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGCGAAAGCAGCGGAAACCCCAACAGTTAATCTCGGACTGCGAAGGTCCCAGCGCGTCTGAGA
  ACGGTGATGCTAGCGAGGAGGATCACCCCCAAGTCTGTGCCAAGTGCTGCGCACAATTCACTGACCCAAC
  TGAATTCCTCGCCCACCAGAACGCATGTTCTACTGACCCTCCTGTAATGGTGATAATTGGGGGCCAGGAG
  AACCCCAACAACTCTTCGGCCTCCTCTGAACCCCGGCCTGAGGGTCACAATAATCCTCAGGTCATGGACA
  CAGAGCATAGCAACCCCCCAGATTCTGGGTCCTCCGTGCCCACGGATCCCACCTGGGGCCCAGAGAGGAG
  AGGAGAGGAGTCTCCAGGGCATTTCCTGGTCGCTGCCACAGGTACAGCGGCTGGGGGAGGCGGGGGCCTG
  ATCTTGGCCAGTCCCAAGCTGGGAGCAACCCCATTACCTCCAGAATCGACCCCTGCACCCCCTCCTCCTC
  CACCACCCCCTCCGCCCCCAGGGGTAGGCAGTGGCCACTTGAATATCCCCCTGATCTTGGAAGAGCTACG
  GGTGCTGCAGCAGCGGCAGATCCATCAGATGCAGATGACTGAGCAAATCTGCAGGCAGGTGCTGTTGCTT
  GGCTCCTTAGGCCAGACGGTGGGTGCCCCTGCCAGTCCCTCAGAGCTACCTGGGACAGGGACTGCCTCTT
  CCACCAAGCCCCTACTACCCCTCTTCAGCCCCATCAAGCCTGTCCAAACCAGCAAGACACTGGCATCTTC
  CTCCTCCTCCTCCTCTTCCTCTTCAGGGGCAGAAACGCCCAAGCAGGCCTTCTTCCACCTTTACCACCCA
  CTGGGGTCACAGCATCCTTTCTCTGCTGGAGGGGTTGGGCGAAGCCACAAACCCACCCCTGCCCCTTCCC
  CAGCCTTGCCAGGCAGCACAGATCAGCTGATTGCCTCGCCTCATCTGGCATTCCCAAGCACCACGGGACT
  ACTGGCAGCACAGTGTCTTGGGGCAGCCCGAGGCCTTGAGGCCACTGCCTCCCCAGGGCTCCTGAAGCCA
  AAGAATGGAAGTGGTGAGCTGAGCTACGGAGAAGTGATGGGTCCCTTGGAGAAGCCTGGTGGAAGGCACA
  AATGCCGCTTCTGTGCCAAAGTATTTGGCAGTGACAGTGCCCTGCAGATCCACCTTCGTTCCCACACGGG
  TGAGAGGCCCTATAAGTGCAATGTCTGTGGAAACCGTTTTACCACCCGTGGCAACCTCAAAGTGCATTTC
  CACCGGCATCGTGAGAAGTACCCACATGTGCAGATGAACCCACACCCAGTACCAGAGCACCTAGACTATG
  TCATTACCAGCAGTGGCTTGCCTTATGGTATGTCCGTGCCACCAGAGAAGGCCGAGGAGGAGGCAGCCAC
  TCCAGGTGGAGGGGTTGAGCGCAAGCCTCTGGTGGCCTCCACAACAGCACTCAGTGCCACAGAGAGCCTG
  ACTCTGCTCTCCACCAGTGCAGGCACAGCCACGGCTCCAGGACTCCCTGCTTTCAATAAGTTTGTGCTCA
  TGAAAGCAGTGGAACCCAAGAATAAAGCTGATGAAAACACCCCCCCAGGGAGTGAGGGCTCAGCCATCAG
  TGGAGTGGCAGAAAGTAGCACGGCAACTCGCATGCAACTAAGTAAGTTGGTGACTTCACTACCAAGCTGG
  GCACTGCTTACCAACCACTTCAAGTCCACTGGCAGCTTCCCCTTCCCCTATGTGCTAGAGCCCTTGGGGG
  CCTCACCCTCTGAGACATCAAAGCTGCAGCAACTGGTAGAAAAGATTGACCGGCAAGGAGCTGTGGCGGT
  GACCTCAGCTGCCTCAGGAGCCCCCACCACCTCTGCCCCTGCACCTTCATCCTCAGCCTCTTCTGGACCT
  AACCAGTGTGTCATCTGTCTCCGAGTGCTTAGCTGTCCTCGGGCCCTACGCCTTCATTATGGCCAACATG
  GAGGTGAGAGGCCCTTCAAATGCAAAGTGTGTGGCAGAGCCTTCTCCACCAGGGGTAATCTGCGTGCACA
  TTTCGTGGGCCACAAGGCCAGTCCAGCTGCCCGGGCACAGAATTCCTGCCCCATCTGCCAGAAGAAGTTC
  ACCAATGCTGTCACTCTGCAGCAGCATGTCCGGATGCACCTGGGGGGCCAGATCCCCAACGGTGGTACTG
  CACTCCCTGAAGGTGGAGGAGCTGCTCAGGAGAATGGCTCCGAGCAATCTACAGTCTCCGGGGCAGGGAG
  TTTCCCCCAGCAGCAGTCCCAGCAGCCATCACCGGAAGAGGAGTTGTCTGAGGAGGAGGAAGAGGAGGAT
  GAGGAAGAAGAGGAAGATGTGACTGATGAAGATTCCCTGGCAGGGAGAGGCTCAGAGAGTGGAGGTGAGA
  AGGCAATATCAGTGAGAGGTGATTCAGAAGAGGCATCTGGGGCAGAGGAGGAGGTGGGGACAGTGGCGGC
  AGCAGCCACAGCTGGGAAGGAGATGGACAGTAATGAGAAAACTACTCAACAGTCTTCTTTGCCACCACCA
  CCACCACCTGACAGCCTGGATCAGCCTCAGCCAATGGAGCAGGGAAGCAGTGGTGTTTTAGGAGGCAAGG
  AAGAGGGGGGCAAACCGGAGAGAAGCTCAAGTCCGGCATCAGCACTCACCCCAGAAGGGGAAGCCACCAG
  CGTGACCTTGGTAGAGGAGCTGAGCCTGCAGGAGGCAATGAGAAAGGAGCCAGGAGAGAGCAGCAGCAGA
  AAGGCCTGCGAAGTGTGTGGCCAGGCCTTTCCCTCCCAGGCAGCTCTGGAGGAGCATCAGAAGACCCACC
  CCAAGGAGGGGCCGCTCTTCACTTGTGTTTTCTGCAGGCAGGGCTTTCTTGAGCGGGCTACCCTCAAGAA
  GCATATGCTCCTGGCACACCACCAGGTACAGCCCTTTGCCCCCCATGGCCCTCAGAATATTGCTGCTCTT
  TCTCTAGTCCCTGGCTGTTCGCCTTCCATCACCTCCACAGGGCTCTCCCCCTTTCCCCGAAAAGATGACC
  CCACGATCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218496 representing NM_005407
  Red=Cloning site Green=Tags(s)
  
  MSRRKQRKPQQLISDCEGPSASENGDASEEDHPQVCAKCCAQFTDPTEFLAHQNACSTDPPVMVIIGGQE
  NPNNSSASSEPRPEGHNNPQVMDTEHSNPPDSGSSVPTDPTWGPERRGEESPGHFLVAATGTAAGGGGGL
  ILASPKLGATPLPPESTPAPPPPPPPPPPPGVGSGHLNIPLILEELRVLQQRQIHQMQMTEQICRQVLLL
  GSLGQTVGAPASPSELPGTGTASSTKPLLPLFSPIKPVQTSKTLASSSSSSSSSSGAETPKQAFFHLYHP
  LGSQHPFSAGGVGRSHKPTPAPSPALPGSTDQLIASPHLAFPSTTGLLAAQCLGAARGLEATASPGLLKP
  KNGSGELSYGEVMGPLEKPGGRHKCRFCAKVFGSDSALQIHLRSHTGERPYKCNVCGNRFTTRGNLKVHF
  HRHREKYPHVQMNPHPVPEHLDYVITSSGLPYGMSVPPEKAEEEAATPGGGVERKPLVASTTALSATESL
  TLLSTSAGTATAPGLPAFNKFVLMKAVEPKNKADENTPPGSEGSAISGVAESSTATRMQLSKLVTSLPSW
  ALLTNHFKSTGSFPFPYVLEPLGASPSETSKLQQLVEKIDRQGAVAVTSAASGAPTTSAPAPSSSASSGP
  NQCVICLRVLSCPRALRLHYGQHGGERPFKCKVCGRAFSTRGNLRAHFVGHKASPAARAQNSCPICQKKF
  TNAVTLQQHVRMHLGGQIPNGGTALPEGGGAAQENGSEQSTVSGAGSFPQQQSQQPSPEEELSEEEEEED
  EEEEEDVTDEDSLAGRGSESGGEKAISVRGDSEEASGAEEEVGTVAAAATAGKEMDSNEKTTQQSSLPPP
  PPPDSLDQPQPMEQGSSGVLGGKEEGGKPERSSSPASALTPEGEATSVTLVEELSLQEAMRKEPGESSSR
  KACEVCGQAFPSQAALEEHQKTHPKEGPLFTCVFCRQGFLERATLKKHMLLAHHQVQPFAPHGPQNIAAL
  SLVPGCSPSITSTGLSPFPRKDDPTIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005407
• ORF Size: 3021 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005407.1, NM_005407.2, NP_005398.1, NP_005398.2
• RefSeq Size: 4931 bp
• RefSeq ORF: 3024 bp
• Locus ID: 6297
• Cytogenetics: 14q11.2
• Protein Families: Druggable Genome
• MW: 105.1 kDa
• Gene Summary: 'This gene encodes a protein containing multiple zinc finger domains. The encoded protein functions in optical fissure closure during development of the eye in the embryo. Mutations in this gene are associated with ocular coloboma. [provided by RefSeq, Jul 2016]'