LAMC2 (NM_018891) Human Tagged ORF Clone
CAT#: RC218516L4
- LentiORF®
Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, mGFP tagged
"NM_018891" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | LAMC2 |
Synonyms | B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC218516).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_018891 |
ORF Size | 3333 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018891.1, NP_061486.1 |
RefSeq Size | 4522 bp |
RefSeq ORF | 3336 bp |
Locus ID | 3918 |
Cytogenetics | 1q25.3 |
Domains | LamB, EGF_Lam |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
MW | 121.6 kDa |
Gene Summary | 'Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two tr' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC315189 | LAMC2 (untagged)-Human laminin, gamma 2 (LAMC2), transcript variant 2 |
USD 1,100.00 |
|
RC218516 | LAMC2 (Myc-DDK-tagged)-Human laminin, gamma 2 (LAMC2), transcript variant 2 |
USD 600.00 |
|
RG218516 | LAMC2 (GFP-tagged) - Human laminin, gamma 2 (LAMC2), transcript variant 2 |
USD 660.00 |
|
RC218516L1 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, Myc-DDK-tagged |
USD 984.00 |
|
RC218516L2 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, mGFP tagged |
USD 800.00 |
|
RC218516L3 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, Myc-DDK-tagged |
USD 800.00 |
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