SLIT1 (NM_003061) Human Tagged ORF Clone

CAT#: RC218562

SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLIT1
• Synonyms: MEGF4; SLIL1; SLIT-1; SLIT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218562 representing NM_003061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGACTCCCGGGTGGGGGTCCTCGGCGGGGCCGGTCCGGCCGGAGCTCTGGCTGCTGCTGTGGG
  CAGCCGCGTGGCGCCTGGGTGCCTCGGCGTGCCCCGCCCTCTGCACCTGCACCGGAACCACGGTGGACTG
  CCACGGCACGGGGCTGCAGGCCATTCCCAAGAATATACCTCGGAACACCGAGCGCCTGGAACTCAATGGC
  AACAACATCACTCGGATCCATAAGAATGACTTTGCGGGGCTCAAGCAGCTGCGGGTGCTGCAGCTGATGG
  AGAACCAGATTGGAGCAGTGGAACGTGGTGCTTTTGATGACATGAAGGAGCTGGAGCGGCTGCGACTGAA
  CCGAAACCAGCTGCACATGTTACCGGAACTGCTGTTCCAGAACAACCAGGCTTTGTCAAGACTGGACTTG
  AGTGAGAACGCCATCCAGGCCATCCCCAGGAAAGCTTTTCGGGGAGCTACGGACCTTAAAAATTTACGGC
  TGGACAAGAACCAGATCAGCTGCATTGAGGAAGGGGCCTTCCGTGCTCTGCGGGGGCTGGAGGTGCTGAC
  CCTGAACAACAACAATATCACCACCATCCCCGTGTCCAGCTTCAACCATATGCCCAAGCTACGGACCTTC
  CGCCTGCACTCCAACCACCTGTTTTGCGACTGCCACCTGGCCTGGCTCTCGCAGTGGCTGAGGCAGCGGC
  CAACCATCGGGCTCTTCACCCAGTGCTCGGGCCCAGCCAGCCTGCGTGGCCTCAATGTGGCAGAGGTCCA
  GAAGAGTGAGTTCAGCTGCTCAGGCCAGGGAGAAGCGGGGCGCGTGCCCACCTGCACCCTGTCCTCCGGC
  TCCTGCCCGGCCATGTGCACCTGCAGCAATGGCATCGTGGACTGTCGTGGAAAAGGCCTCACTGCCATCC
  CGGCCAACCTGCCCGAGACCATGACGGAGATACGCCTGGAGCTGAACGGCATCAAGTCCATCCCTCCTGG
  AGCCTTCTCACCCTACAGAAAGCTACGGAGGATAGACCTGAGCAACAATCAGATCGCTGAGATTGCACCC
  GACGCCTTCCAGGGCCTCCGCTCCCTGAACTCGCTGGTCCTCTATGGAAACAAGATCACAGACCTCCCCC
  GTGGTGTGTTTGGAGGCCTATACACCCTACAGCTCCTGCTCCTGAATGCCAACAAGATCAACTGCATCCG
  GCCCGATGCCTTCCAGGACCTGCAGAACCTCTCACTGCTCTCCCTGTATGACAACAAGATCCAGAGCCTC
  GCCAAGGGCACTTTCACCTCCCTGCGGGCCATCCAGACTCTGCACCTGGCCCAGAACCCTTTCATTTGCG
  ACTGTAACCTCAAGTGGCTGGCAGACTTCCTGCGCACCAATCCCATCGAGACGAGTGGTGCCCGCTGTGC
  CAGTCCCCGGCGCCTCGCCAACAAGCGCATCGGGCAGATCAAGAGCAAGAAGTTCCGGTGCTCAGCCAAA
  GAGCAGTACTTCATTCCAGGCACGGAGGATTACCAGCTGAACAGCGAGTGCAACAGCGACGTGGTCTGTC
  CCCACAAGTGCCGCTGTGAGGCCAACGTGGTGGAGTGCTCCAGCCTGAAGCTCACCAAGATCCCTGAGCG
  CATCCCCCAGTCCACGGCAGAACTGCGATTGAATAACAATGAGATTTCCATCCTGGAGGCCACTGGGATG
  TTTAAAAAACTTACACATCTGAAGAAAATCAATCTGAGCAACAACAAGGTGTCAGAAATTGAAGATGGGG
  CCTTCGAGGGCGCAGCCTCTGTGAGCGAGCTGCACCTAACTGCCAACCAGCTGGAGTCCATCCGGAGCGG
  CATGTTCCGGGGTCTGGATGGCTTGAGGACCCTAATGCTGCGGAACAACCGCATCAGCTGCATCCACAAC
  GACAGCTTCACGGGCCTGCGCAACGTCCGGCTCCTCTCGCTCTACGACAACCAGATCACCACCGTATCCC
  CAGGAGCCTTCGACACCCTCCAGTCCCTCTCCACACTGAATCTCCTGGCCAACCCTTTCAACTGCAACTG
  CCAGCTGGCCTGGCTAGGAGGCTGGCTACGGAAGCGCAAGATCGTGACGGGGAACCCGCGATGCCAGAAC
  CCTGACTTTTTGCGGCAGATTCCCCTGCAGGACGTGGCCTTCCCTGACTTCAGGTGTGAGGAAGGCCAGG
  AGGAGGGGGGCTGCCTGCCCCGCCCACAGTGCCCACAGGAGTGCGCCTGCCTGGACACCGTGGTCCGATG
  CAGCAACAAGCACCTGCGGGCCCTGCCCAAGGGCATTCCCAAGAATGTCACAGAACTCTATTTGGACGGG
  AACCAGTTCACGCTGGTTCCGGGACAGCTGTCTACCTTCAAGTACCTGCAGCTCGTGGACCTGAGCAACA
  ACAAGATCAGTTCCTTAAGCAATTCCTCCTTCACCAACATGAGCCAGCTGACCACTCTGATCCTCAGCTA
  CAATGCCCTGCAGTGCATCCCGCCTTTGGCCTTCCAGGGACTCCGCTCCCTGCGCCTGCTGTCTCTCCAC
  GGCAATGACATCTCCACCCTCCAAGAGGGCATCTTTGCAGACGTGACCTCCCTGTCTCACCTGGCCATTG
  GTGCCAACCCCCTATACTGTGACTGCCACCTCCGCTGGCTGTCCAGCTGGGTGAAGACTGGCTACAAGGA
  ACCGGGCATTGCTCGTTGTGCTGGGCCCCAGGACATGGAGGGCAAGCTGCTCCTCACCACGCCTGCCAAG
  AAGTTTGAATGCCAAGGTCCTCCAACGCTGGCTGTCCAGGCCAAGTGTGATCTCTGCTTGTCCAGTCCGT
  GCCAGAACCAGGGCACCTGCCACAACGACCCCCTTGAGGTGTACAGGTGCGCCTGCCCCAGCGGCTATAA
  GGGTCGAGACTGTGAGGTGTCCCTGAACAGCTGTTCCAGTGGCCCCTGTGAAAATGGGGGCACCTGCCAT
  GCACAGGAGGGCGAGGATGCCCCGTTCACGTGCTCCTGTCCCACCGGCTTTGAAGGACCAACCTGTGGGG
  TGAACACAGATGACTGTGTGGATCATGCCTGTGCCAATGGGGGCGTCTGTGTGGATGGTGTGGGCAACTA
  CACCTGCCAGTGCCCCCTGCAGTATGAGGGAAAGGCCTGTGAGCAGCTGGTGGACTTGTGCTCTCCGGAT
  CTGAACCCATGTCAACACGAGGCCCAGTGTGTGGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCCAG
  GTTATGCAGGTGACAACTGCAGTGAGAACCAGGATGACTGCAGGGACCACCGCTGCCAGAATGGGGCCCA
  GTGTATGGATGAAGTCAACAGCTACTCCTGCCTCTGTGCTGAGGGCTACAGTGGACAGCTCTGTGAGATC
  CCTCCCCATCTGCCTGCCCCCAAGAGCCCCTGTGAGGGGACTGAGTGCCAGAATGGGGCCAACTGTGTGG
  ACCAGGGCAACAGGCCTGTGTGCCAGTGCCTCCCAGGCTTCGGTGGCCCTGAGTGTGAGAAGTTGCTCAG
  TGTCAACTTTGTGGATCGGGACACTTACCTGCAGTTCACTGACCTGCAAAACTGGCCACGGGCCAACATC
  ACGTTGCAGGTCTCCACGGCAGAGGACAATGGGATCCTTCTGTACAACGGGGACAACGACCACATTGCAG
  TTGAGCTGTACCAGGGCCATGTGCGTGTCAGCTACGACCCAGGCAGCTACCCCAGCTCTGCCATCTACAG
  TGCTGAGACGATCAACGATGGGCAATTCCACACCGTTGAGCTGGTTGCCTTTGACCAGATGGTGAATCTC
  TCCATTGATGGCGGGAGCCCCATGACCATGGACAACTTTGGCAAACATTACACGCTCAACAGCGAGGCGC
  CACTCTATGTGGGAGGGATGCCCGTGGATGTCAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAACGG
  CACCGGCTTCCACGGTTGCATCCGAAACCTGTACATCAACAACGAGCTGCAGGACTTCACCAAGACGCAG
  ATGAAGCCAGGCGTGGTGCCAGGCTGCGAACCCTGCCGCAAGCTCTACTGCCTGCATGGCATCTGCCAGC
  CCAATGCCACCCCAGGGCCCATGTGCCACTGCGAGGCTGGCTGGGTGGGCCTGCACTGTGACCAGCCCGC
  TGACGGCCCCTGCCATGGCCACAAGTGTGTCCATGGGCAATGCGTGCCCCTCGACGCTCTTTCCTACAGC
  TGCCAGTGCCAGGATGGGTACTCGGGGGCACTGTGCAACCAGGCCGGGGCCCTGGCAGAGCCCTGCAGAG
  GCCTGCAGTGCCTGCATGGCCACTGCCAGGCCTCAGGCACCAAGGGGGCACACTGTGTGTGTGACCCCGG
  CTTTTCGGGCGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCAGGTC
  CAGAGGGGCTATGCCATCTGCCAGACCACGCGCCCCCTGTCATGGGTGGAGTGCCGGGGCTCGTGCCCAG
  GCCAGGGCTGCTGCCAGGGCCTTCGGCTGAAGCGGAGGAAGTTCACCTTTGAGTGCAGCGATGGGACCTC
  TTTTGCCGAGGAGGTGGAAAAGCCCACCAAGTGTGGCTGTGCCCTCTGCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218562 representing NM_003061
  Red=Cloning site Green=Tags(s)
  
  MALTPGWGSSAGPVRPELWLLLWAAAWRLGASACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNG
  NNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLHMLPELLFQNNQALSRLDL
  SENAIQAIPRKAFRGATDLKNLRLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTF
  RLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAGRVPTCTLSSG
  SCPAMCTCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAP
  DAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL
  AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQIKSKKFRCSAK
  EQYFIPGTEDYQLNSECNSDVVCPHKCRCEANVVECSSLKLTKIPERIPQSTAELRLNNNEISILEATGM
  FKKLTHLKKINLSNNKVSEIEDGAFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHN
  DSFTGLRNVRLLSLYDNQITTVSPGAFDTLQSLSTLNLLANPFNCNCQLAWLGGWLRKRKIVTGNPRCQN
  PDFLRQIPLQDVAFPDFRCEEGQEEGGCLPRPQCPQECACLDTVVRCSNKHLRALPKGIPKNVTELYLDG
  NQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH
  GNDISTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPQDMEGKLLLTTPAK
  KFECQGPPTLAVQAKCDLCLSSPCQNQGTCHNDPLEVYRCACPSGYKGRDCEVSLNSCSSGPCENGGTCH
  AQEGEDAPFTCSCPTGFEGPTCGVNTDDCVDHACANGGVCVDGVGNYTCQCPLQYEGKACEQLVDLCSPD
  LNPCQHEAQCVGTPDGPRCECMPGYAGDNCSENQDDCRDHRCQNGAQCMDEVNSYSCLCAEGYSGQLCEI
  PPHLPAPKSPCEGTECQNGANCVDQGNRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANI
  TLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVAFDQMVNL
  SIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTGFHGCIRNLYINNELQDFTKTQ
  MKPGVVPGCEPCRKLYCLHGICQPNATPGPMCHCEAGWVGLHCDQPADGPCHGHKCVHGQCVPLDALSYS
  CQCQDGYSGALCNQAGALAEPCRGLQCLHGHCQASGTKGAHCVCDPGFSGELCEQESECRGDPVRDFHQV
  QRGYAICQTTRPLSWVECRGSCPGQGCCQGLRLKRRKFTFECSDGTSFAEEVEKPTKCGCALCA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003061
• ORF Size: 4602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003061.1, NM_003061.2, NP_003052.1, NP_003052.2
• RefSeq Size: 5094 bp
• RefSeq ORF: 4605 bp
• Locus ID: 6585
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 167.7 kDa
• Gene Summary: ''