DGLUCY (NM_001102368) Human Tagged ORF Clone

CAT#: RC218607

C14orf159 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 159 (C14orf159), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY420135) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218607 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGLUCY
• Synonyms: C14orf159
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218607 representing NM_001102368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTCACACTCCACCTGAGGTCCCGCCTTCCCTCTGCCATAAGGAGTTTGATTCTACAAAAGAAAC
  CAAACATCAGAAATACATCCAGCATGGCTGGAGAGCTCCGACCAGCCAGCCTGGTGGTCCTGCCCAGGTC
  CCTTGCTCCAGCTTTTGAAAGATTCTGCCAGGTCAACACTGGTCCTCTACCCCTGCTGGGCCAGAGTGAG
  CCAGAAAAGTGGATGCTGCCCCCTCAAGGTGCTATCTCAGAGACCAGGATGGGCCATCCCCAGTTCTGGA
  AATACGAGTTCGGTGCCTGCACCGGCAGCCTGGCTTCGCTGGAGCAGTACTCGGAGCAGCTGAAGGACAT
  GGTGGCCTTCTTCCTGGGCTGCAGCTTCTCCCTGGAGGAGGCCTTGGAGAAAGCGGGGCTCCCCAGAAGA
  GACCCAGCAGGTCACAGCCAGGCGGGTGCATACAAGACAACAGTGCCTTGTGTTACCCATGCTGGCTTCT
  GCTGCCCTCTGGTGGTCACGATGAGGCCCATTCCCAAGGACAAGCTGGAAGGGCTGGTGCGGGCCTGCTG
  CTCCCTCGGAGGTGAGCAGGGGCAACCTGTTCACATGGGCGACCCAGAACTGTTGGGAATCAAAGAGCTT
  TCCAAACCTGCCTACGGGGATGCCATGGTGTGTCCCCCAGGGGAGGTTCCAGTGTTCTGGCCTTCTCCGC
  TGACCAGTCTCGGAGCTGTCAGCAGCTGTGAGACCCCACTGGCTTTTGCCAGCATCCCAGGCTGCACAGT
  TATGACTGACCTGAAGGATGCAAAGGCTCCACCTGGTTGTCTCACCCCAGAGAGAATTCCAGAGGTCCAT
  CACATTTCCCAAGATCCTCTGCACTACAGCATCGCGTCAGTCTCTGCTTCTCAGAAGATCAGAGAACTAG
  AGTCTATGATCGGCATAGACCCAGGGAACCGGGGGATTGGGCACCTGCTCTGTAAAGATGAGCTGCTGAA
  GGCCTCTCTCTCGCTGTCCCATGCCCGCTCAGTGCTCATCACCACTGGGTTCCCCACACATTTCAATCAT
  GAGCCTCCAGAAGAGACAGATGGCCCACCAGGAGCTGTTGCTCTGGTTGCCTTCCTGCAGGCCTTGGAGA
  AGGAGGTCGCCATAATCGTTGACCAGAGAGCCTGGAACTTGCACCAGAAGATTGTTGAAGATGCTGTTGA
  GCAAGGTGTTCTGAAGACGCAGATCCCGATATTAACTTACCAAGGTGGATCAGTGGAAGCTGCTCAGGCA
  TTCCTGTGCAAAAATGGGGACCCGCAGACACCTAGATTTGACCACCTGGTGGCCATAGAGCGTGCCGGAA
  GAGCTGCTGATGGCAATTACTACAATGCAAGGAAGATGAACATCAAGCACTTGGTTGACCCCATTGACGA
  TCTTTTTCTTGCTGCGAAGAAGATTCCTGGAATCTCATCAACTGGAGTCGGTGATGGAGGCAACGAGCTT
  GGGATGGGTAAAGTCAAGGAGGCTGTGAGGAGGCACATACGGCACGGGGATGTCATCGCCTGCGACGTGG
  AGGCTGACTTTGCCGTCATTGCTGGTGTTTCTAACTGGGGAGGCTATGCCCTGGCCTGCGCACTCTACAT
  CCTGTACTCATGTGCTGTCCACAGTCAGTACCTGAGGAAAGCAGTCGGACCCTCCAGGGCACCTGGAGAT
  CAGGCCTGGACTCAGGCCCTCCCGTCGGTCATTAAGGAAGAAAAAATGCTGGGCATCTTGGTGCAGCACA
  AAGTCCGGAGTGGCGTCTCGGGCATCGTGGGCATGGAGGTGGATGGGCTGCCCTTCCACAACACCCACGC
  CGAGATGATCCAGAAGCTGGTGGACGTCACCACGGCACAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218607 representing NM_001102368
  Red=Cloning site Green=Tags(s)
  
  MPFTLHLRSRLPSAIRSLILQKKPNIRNTSSMAGELRPASLVVLPRSLAPAFERFCQVNTGPLPLLGQSE
  PEKWMLPPQGAISETRMGHPQFWKYEFGACTGSLASLEQYSEQLKDMVAFFLGCSFSLEEALEKAGLPRR
  DPAGHSQAGAYKTTVPCVTHAGFCCPLVVTMRPIPKDKLEGLVRACCSLGGEQGQPVHMGDPELLGIKEL
  SKPAYGDAMVCPPGEVPVFWPSPLTSLGAVSSCETPLAFASIPGCTVMTDLKDAKAPPGCLTPERIPEVH
  HISQDPLHYSIASVSASQKIRELESMIGIDPGNRGIGHLLCKDELLKASLSLSHARSVLITTGFPTHFNH
  EPPEETDGPPGAVALVAFLQALEKEVAIIVDQRAWNLHQKIVEDAVEQGVLKTQIPILTYQGGSVEAAQA
  FLCKNGDPQTPRFDHLVAIERAGRAADGNYYNARKMNIKHLVDPIDDLFLAAKKIPGISSTGVGDGGNEL
  GMGKVKEAVRRHIRHGDVIACDVEADFAVIAGVSNWGGYALACALYILYSCAVHSQYLRKAVGPSRAPGD
  QAWTQALPSVIKEEKMLGILVQHKVRSGVSGIVGMEVDGLPFHNTHAEMIQKLVDVTTAQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102368
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001102368.1, NM_001102368.2, NP_001095838.1
• RefSeq Size: 2763
• RefSeq ORF: 1866
• Locus ID: 80017
• MW: 66.7 kDa